Effects of diludine supplementation on growth performance, liver antioxidant enzyme activities and muscular trace elements of rainbow trout (Oncorhynchus mykiss) juveniles at a low water temperature

Abstract

Diludine is used as an effective agent reducing mutagenic effects of environmental pollutants, increasing productivity and protecting reproductive system of parental individuals and quality of their offspring. A 10‐week study was conducted to evaluate potential effects of diludine on rainbow trout (Oncorhynchus mykiss) juveniles growth performance, feed utilization, survival rate, liver antioxidant enzyme activities (superoxide dismutase, SOD, http://www.chem.qmul.ac.uk/iubmb/enzyme/EC1/15/1/1.html; catalase, CAT, http://www.chem.qmul.ac.uk/iubmb/enzyme/EC1/11/1/16.html; glutathione peroxidase, GPx, http://www.chem.qmul.ac.uk/iubmb/enzyme/EC1/11/1/9.html; glutathione reductase, GR, http://www.chem.qmul.ac.uk/iubmb/enzyme/EC1/6/4/2.html; glucose‐6‐phosphate dehydrogenase, G6PD, http://www.chem.qmul.ac.uk/iubmb/enzyme/EC1/1/1/49.html and glutathione S‐transferase, GST, http://www.chem.qmul.ac.uk/iubmb/enzyme/EC1/5/1/18.html), the levels of malondialdehyde (MDA) as a lipid peroxidation marker and muscular trace elements at a low water temperature (9 °C). Diludine was added at 0.2, 0.5 and 1 g kg−1 to a fish meal–based control diet, and each diet was fed to triplicate groups of rainbow trout juveniles. After 10 weeks, there were no differences in survival rate between fish fed experimental diets (P > 0.05). It was observed that a significant improvability existed for both growth and feed utilization in fish fed diets supplemented with diludine (P < 0.05). Antioxidant enzymes showed significantly increased activity in liver tissues (P < 0.05) and then a decrease to initial activity levels during the experimental time. (GPx activity was not determined in the juvenile livers.) SOD and GST activities in liver of fish fed the diets with diludine tended to be higher and GR activity tended to be lower than that in fish fed the basal diet. However, in general, it was determined that different concentration of diludine did not affect the studied antioxidant enzyme activities except G6PD in the liver of juveniles fed the diet with 1 g kg−1 diludine. On the other hand, the significant reduction was found in MDA levels in the fish fed the diets with diludine compared with basal diet on the 14th and 28th days of experiment. It was also observed that Cu, Fe, Mg, Zn and Se levels were significantly higher in the juveniles fed the diet with 1 g kg−1 diludine than those fed the basal diet. In conclusion, the results of this trial indicate that 1 g kg−1 dietary supplementation with diludine seems to be most positive for rainbow trout production in cold‐water adaptation.

Growth performance and antioxidant enzyme activities in rainbow trout (Oncorhynchus mykiss) juveniles fed diets supplemented with sage, mint and thyme oils

“…According to Hisar et al (2012), higher GST activities in liver of fish fed the diets with diludine tended to be higher. Similarly, in our study, GST activities in liver of fish fed the diets containing mint (all doses) and thyme (T500 and T1000) were increased.…”

Effects of grapeVitis viniferaseed oil supplementation on growth, survival, fatty acid profiles, antioxidant contents and blood parameters in rainbow troutOncorhynchus mykiss

“…Conversely, Hisar et al. () observed an increase in GST activity in rainbow trout when they supplemented with a diuretic. Sönmez, Aydın, Hisar, Aydın and Aras Hisar () obtained similar results in rainbow trout juvenile supplemented with mint and thyme.…”

The extracts ofAngelica sinensisinhibit lipid oxidation in fish erythrocytes and improve growth, digestive, absorptive and antioxidant capacity in juvenile Jian carp (Cyprinus carpiovar. Jian)

“…The content of Hb and Met‐Hb was determined by the commercial kit (Jiancheng, Nanjing, China; Li et al, ). The activities of SOD, GPx, CAT and GR were determined by the method of Hisar et al (). The activities of trypsin, lipase and amylase were determined by using the method of Ling et al ().…”