Effects of endotoxin lipopolysaccharide administration on the somatotropic axis

Soto, L; Martin, AI; Millán, S.; Vara, Elena; López-Calderón, Asunción

doi:10.1677/joe.0.1590239

Cited by 70 publications

(70 citation statements)

References 44 publications

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“…Studies have reported that inflammatory cytokines can directly act on central nervous system, reducing the secretion of the growth promoting hormone such as GH and IGF-1 (Hasselgren 1993;Soto et al 1998), and eventually affect the growth performance of animals. In this study, LPS stimulation significantly reduced the serum GH and IGF-1 content, which in line with the previous studies.…”

Section: Growth Performancementioning

confidence: 99%

DietaryArtemisia ordosicaextract alleviating immune stress in broilers exposed to lipopolysaccharide

Zhang

Shi

et al. 2017

Italian Journal of Animal Science

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This study was conducted to investigate the effect of Artemisia ordosica extract (AOE) on broilers challenged with lipopolysaccharide (LPS). 96 one-day-old Arbour Acres broilers were assigned in 2 Â 2 factorial design, including two dietary treatments (0 or 1000 mg/kg AOE) and two immunological challenge (saline or LPS). on d 14, 16, 18 and 20, broilers were injected intra-abdominally with LPS solution (the LPS was dissolved in sterile saline at a concentration of 100 lg/mL) at 500 lg/kg of base weight, or an equivalent amount of sterile saline. Blood samples were collected on d 21 and 28. During LPS-challenged periods (days 15-21), AOE alleviated the compromised average daily gain and average daily feed intake (p < .05) in broilers challenged with LPS. On day 21, the LPS challenge increased (p < .05) serum adrenocorticotropic hormone, corticosterone, interleukin-1, interleukin-2, interleukin-6, immunoglobulin G and immunoglobulin A, decreased (p < .05) the content of serum growth hormone and insulin-like growth factor-1. However, diet supplemented with AOE reduced the elevation of serum corticosterone (p ¼ .054), interleukin-2 (p < .05), immunoglobulin A (p < .05) and immunoglobulin G (p ¼ .079) caused by LPS on day 21. After a week's recovery, on d 28, AOE reduced the serum interleukin-6 content (p < .05). It may be that AOE exert its beneficial effect on broilers challenged with LPS by lessening the inflammatory cytokines and stress hormone, weakening the over activated immune system and finally, improving the growth performance.ARTICLE HISTORY

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Section: Growth Performancementioning

confidence: 99%

DietaryArtemisia ordosicaextract alleviating immune stress in broilers exposed to lipopolysaccharide

Zhang

Shi

et al. 2017

Italian Journal of Animal Science

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“…Endotoxin administration in rats increases plasma concentrations of corticosterone, whereas it decreases circulating IGF-I and its gene expression in the liver (Soto et al 1998, Defalque et al 1999. The adult liver is the main source of circulating IGF-I and its main serum-binding protein, IGF-binding protein-3 (IGFBP-3), which are synthesized by different cell populations.…”

Section: Introductionmentioning

confidence: 99%

Inactivation of Kupffer cells by gadolinium administration prevents lipopolysaccharide-induced decrease in liver insulin-like growth factor-I and IGF-binding protein-3 gene expression

Granado¹,

Martín²,

Priego³

et al. 2006

Journal of Endocrinology

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Gram-negative bacterial infection or treatment of animals with bacterial lipopolysaccharide (LPS) induces a catabolic state with proteolysis, liver injury and an inhibition of the insulin-like growth factor-I (IGF-I) system. The purpose of this work was to elucidate the role of Kupffer cells in LPS-induced inhibition of the IGF-I/IGF-binding protein-3 (IGFBP-3) system. Adult male Wistar rats were either pretreated with the Kupffer cell inhibitor gadolinium chloride (10 mg/kg, i.v., 24 h prior to LPS exposure) or saline vehicle. Rats received two i.p. injections of 1 mg/kg LPS (at 17:30 and 08:30 h the following day) and were killed 4 h after the second injection. LPS administration induced a significant decrease in body weight and in serum concentrations of IGF-I and IGFBP-3 (P<0·01), as well as in their gene expression in the liver. LPS-injected rats had increased serum concentrations of ACTH, corticosterone (P<0·05), tumour necrosis factor-(TNF-) and nitrites (P<0·01). Pretreatment of the animals with gadolinium chloride blocked the inhibitory effect of LPS on body weight, and on serum concentrations of IGF-I, IGFBP-3 and nitrites, as well as growth hormone receptor (GHR), IGF-I and IGFBP-3 gene expression in the liver. In contrast, gadolinium chloride administration did not modify the stimulatory effect of LPS on serum concentrations of ACTH, corticosterone and TNF-. These results suggest that Kupffer cells are important mediators in the inhibitory effect of LPS on GHR, IGF-I and IGFBP-3 gene expression in the liver, leading to a decrease in serum concentrations of IGF-I and IGFBP-3.

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“…Serum IGF-I was removed from IGFBPs by an acidethanol extraction and was measured by a double-antibody RIA (Soto et al 1998). The IGF-I antiserum (UB-2495) was a gift from Drs Underwood and Van Wyk, and was distributed by the Hormone Distribution Program of NIDDK (Bethesda, MD, USA) through the National Hormone and Pituitary Program.…”

Section: Hormone Measurementsmentioning

confidence: 99%

“…Western blots were prepared as previously described (Soto et al 1998). Blood serum proteins were separated by 12·5% SDS-PAGE under non-reducing conditions and blotted onto nitrocellulose membranes (Hybond-C-extra; Amersham, Little Chalfont, Bucks, UK) by means of a semidry electrophoretic transfer cell (Bio-Rad, Hercules, CA, USA).…”

Section: Western Ligand Blotmentioning

confidence: 99%

“…Sepsis can be induced experimentally by administering lipopolysaccharide (LPS), a component of the wall of gram-negative bacteria. LPS administration decreases circulating levels of IGF-I both in humans and in experimental animals (Fan et al 1994, Soto et al 1998. We have previously observed that the decrease in serum IGF-I is associated with a decrease in growth hormone (GH) receptor and IGF-I synthesis in the liver (Priego et al 2002a(Priego et al , 2003a.…”

Section: Introductionmentioning

confidence: 99%

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Dexamethasone administration attenuates the inhibitory effect of lipopolysaccharide on IGF-I and IGF-binding protein-3 in adult rats

Priego

Granado²,

Martín³

et al. 2005

Journal of Endocrinology

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The aim of this study was to investigate whether glucocorticoid administration had a beneficial effect on serum concentrations of insulin-like growth factor I (IGF-I) and on IGF-binding protein 3 (IGFBP-3) in rats injected with lipopolysaccharide (LPS). Adult male rats were injected with LPS or saline and pretreated with dexamethasone or saline. Dexamethasone administration decreased growth hormone (GH) receptor and IGF-I mRNA levels in the liver of control rats. LPS decreased GH receptor and IGF-I gene expression in the liver of saline-treated rats but not in the liver of dexamethasone-pretreated rats. In the kidney, GH receptor mRNA levels were not modified by dexamethasone or LPS treatment. However, LPS decreased renal IGF-I gene expression and dexamethasone pretreatment prevented this decrease. Serum concentrations of IGF-I were decreased by LPS, and dexamethasone pretreatment attenuated this effect. The gene expression of IGFBP-3 in the liver and kidney and its circulating levels were decreased by LPS. In control rats dexamethasone increased circulating IGFBP-3 and its gene expression in the liver, and decreased the proteolysis of this protein.Dexamethasone pretreatment attenuated the LPS-induced decrease in IGFBP-3 gene expression in the liver and prevented the LPS-induced decrease in IGFBP-3 gene expression in the kidney. Moreover, dexamethasone pretreatment attenuated the LPS-induced decrease in serum concentrations of IGFBP-3 and decreased the LPSinduced IGFBP-3 proteolysis in serum. In conclusion, dexamethasone pretreatment partially attenuates the inhibitory effect of LPS on serum IGF-I by blocking the decrease of its gene expression in the kidney as well as by attenuating the decrease in serum concentrations of IGFBP-3.

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Effects of endotoxin lipopolysaccharide administration on the somatotropic axis

Cited by 70 publications

References 44 publications

DietaryArtemisia ordosicaextract alleviating immune stress in broilers exposed to lipopolysaccharide

DietaryArtemisia ordosicaextract alleviating immune stress in broilers exposed to lipopolysaccharide

Inactivation of Kupffer cells by gadolinium administration prevents lipopolysaccharide-induced decrease in liver insulin-like growth factor-I and IGF-binding protein-3 gene expression

Dexamethasone administration attenuates the inhibitory effect of lipopolysaccharide on IGF-I and IGF-binding protein-3 in adult rats

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