Effects of enhanced UV-B radiation (280–320 nm) on ribulose-1,5-bisphosphate carboxylase in pea and soybean

Vu, C. V.; Allen, L. H.; Garrard, L.A.

doi:10.1016/0098-8472(84)90014-5

Cited by 82 publications

(48 citation statements)

References 23 publications

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“…This decrease in activity was due to a reduction in the amount of Rubisco present (Table 2) and not simply to a deactivation of the enzyme. UV-B-induced reductions in both Rubisco activity and content have been previously reported in both higher plants (Vu et al 1982;Vu et al 1984;Strid et al 1990;Jordan£'ra/. 1992;He etal 1993;Huang e/a/.…”

Section: Uv-b-induced Limitations To Cop Assimilation 637mentioning

confidence: 99%

“…Reductions in Rubisco activity and stomatal conductance have been implicated as factors limiting CO2 assimilation in leaves exposed to elevated levels of UV-B. Prolonged exposure to UV-B has been demonstrated to result in decreases in both Rubisco activity and content (Vu, Allen & Garrard 1982;Vu, Allen & Garrard 1984;Strid et al 1990;Jordan, Chow & Anderson 1992;He et al 1993;Huang et al 1993;Kulandaivelu & Nedunchezhian 1993;Lingakumar & Kulandaivelu 1993), which may be accompanied by decreases in the mRNA transcripts of both the large and small subunits of Rubisco (Jordan et al 1992;Zhang et al 1994). Exposure to UV-B can also modify the rate of stomatal opening and closing, and reduce the rate of leaf transpiration (Negash 1987;Middleton & Teramura 1993;Day & Vogelmann 1995), although other studies have not implicated stomatal effects as limiting for photosynthesis (Murali & Teramura 1986;Sullivan & Teramura 1989;Teramura etal 1991;Ziska & Teramura 1992).…”

Section: Introductionmentioning

confidence: 99%

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Analysis of limitations to CO₂ assimilation on exposure of leaves of two Brassica napus cultivars to UV‐B

Allen

Mckee

Farage

et al. 1997

Plant Cell & Environment

158

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Apex and Bristol cultivars of oilseed rape {Brassica napus) were irradiated with 0-63 W m~^ of UV-B over 5 d. Analyses of the response of net leaf carbon assimilation to intercellular CO2 concentration were used to examine the potential limitations imposed by stomata, carboxylation velocity and capacity for regeneration of ribulose 1,5-bisphosphate on leaf photosynthesis. Simultaneous measurements of chlorophyll fluorescence were used to estimate the maximum quantum efficiency of photosystem II (PSII) photochemistry, the quantum efficiency of linear electron transport at steady-state photosynthesis, and the light and CO2-saturated rate of linear electron transport. Ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) content and activities were assayed in vitro. In both cultivars the UV-B treatment resulted in decreases in the light-saturated rate of CO2 assimilation, which were accompanied by decreases in carboxylation velocity and Rubisco content and activity. No major effects of UV-B were observed on end-product inhibition and stomatal limitation of photosynthesis or the rate of photorespiration relative to CO2 assimilation. In the Bristol cultivar, photoinhibition of PSII and loss of linear electron transport activity were observed when CO2 assimilation was severely inhibited. However, the Apex cultivar exhibited no major inhibition of PSII photochemistry or linear electron transport as the rate of CO2 assimilation decreased. It is concluded that loss of Rubisco is a primary factor in UV-B inhibition of CO2 assimilation.Key-words: Brassica napus; electron transport; fluorescence; leaf gas exchange; photosynthesis; photosystem II; Rubisco; ultraviolet-B radiation.Abbreviations: A, net CO2 assimilation rate; A^.^^, light-saturated net CO2 assimilation rate; Cj, intercellular CO2 concentration; Fo, F^, E^, minimal, maximal and variable fluorescence yields; F,^', EJ, E^, maximal, variable and steady-state fluorescence yields in a light-adapted state; ^max.RuBP' maximum potential rate of electron transport contributing to RuBP regeneration; imax.psii' rate of PSII electron transport at saturating light and CO2; PPFD, photosynthetically active photon flux density; RuBP, ribulose 1,5-bisphosphate; Rubisco, ribulose 1,5-bisphosphate carboxylase/oxygenase; V^..^^^, maximum carboxylation velocity of Rubisco; ^psn, relative quantum efficiency of PSII photochemistry.

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Section: Uv-b-induced Limitations To Cop Assimilation 637mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Analysis of limitations to CO₂ assimilation on exposure of leaves of two Brassica napus cultivars to UV‐B

Allen

Mckee

Farage

et al. 1997

Plant Cell & Environment

158

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“…However, the relationship of these results to whole plant responses to UV-B radiation is not clear. Vu et al (1984) observed quantitative and qualitative changes of Rubisco from pea and soybean plants exposed to UV-B radiation. The UV-B treatments also increased the UV-B absorbance of extracted pea pigments, suggesting that modification of the UV-B-filtering ability of the intact leaves may not be sufficient to protect the plant from UV-B-induced damage.…”

Section: Effect Of Temperature On Trp Photodegradationmentioning

confidence: 99%

“…Therefore, we investigated the effects of UV-B radiation on the microsomal membranes and Rubisco of cucumber leaves. Although Rubisco was utilized primarily to permit comparisons between soluble and membrane proteins, exposure of pea and soybean plants to UV-B radiation decreased the amount of Rubisco and altered its catalytic properties (Vu et al, 1984). Because Trp is a primary protein chromophore in the near UV and UV-induced tryptophan photoproducts may act as photosensitizers (Walrant and Santus, 1974), in vitro changes in the intrinsic protein Trp fluorescence of cucumber microsomal membranes were measured under a variety of conditions.…”

mentioning

confidence: 99%

Ultraviolet-Induced Photodegradation of Cucumber (Cucumis sativus L.) Microsomal and Soluble Protein Tryptophanyl Residues in Vitro

Caldwell

1993

Plant Physiol.

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l h e in vitro effects of ultraviolet B (280-320 nm) radiation on microsomal membrane proteins and partially purified ribulose bisphosphate carboxylase (Rubisco) from cucumber (Cucumis sativus l.) was investigated by measuring the direct photolytic reduction of tryptophan fluorescence and the formation of fluorescent photooxidation products. Exposure of microsomes and Rubisco to monochromatic 300-nm radiation resulted in the loss of intrinsic tryptophan fluorescence and the production of blue-emitting fluorophores. l h e major produd of tryptophan photolysis was tentatively identified as N-formylkynurenine (N-FK). Even though the rates of tryptophan photodegradation and N-FK formation were similar, the amount of blue fluorescence produced was significantly higher in the microsomes relative to Rubisco. Studies with various free radical scavengers and other modifiers indicated that tryptophan photodegradation requires oxygen and that the subsequent formation of N-FK may involve readive oxygen species. l h e optimum wavelengths for loss of typtophan fluorescence were 290 nm for the microsomes and 280 nm for Rubisco. The temperature dependence of tryptophan fluorescence and rate of tryptophan photodegradation indicated an alteration in the cucumber microsoma1 membranes at about 24"C, which influenced protein structure and tryptophan photosensitivity.

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“…(Jordan et al, 1992). Rubisco 활성과 함량 감소에 대한 UV-B의 영향에 대한 연구는 다양한 생물종에서 연구되었다 (Vu et al, 1982;Vu et al, 1984;Strid et al, 1990;Jordan et al, 1992;He et al, 1993;He et al, 1994;Huang et al, 1993;Kulandaivelu and Nedunchezhian, 1993). .…”

Section: Figunclassified

Changes in photosynthesis and carbohydrate synthesis in response to elevated UV-B environment

윤혜진¹,

성좌경²,

이수연³

et al. 2014

Korean Journal of Agricultural Science

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:The ozone depletion has caused plants to be exposed to an increased penetration of solar ultraviolet-B (UV-B) radiation. Enhanced UV-B radiation may have influence on biological functions of plant in many aspects including inhibition of photosynthesis. It is evident that UV-B can potentially impair the performance of all three main component processes of photosynthesis, the photophosphorylation reactions of the thylakoid membrane, the CO2-fixation reactions of the Calvin cycle and stomatal control of CO2 supply. Owing to these depressed reactions, the production and allocation of carbohydrates might be markedly affected, and therefore, the growth and development of plant are distinctly reduced. In this review paper, we provide basic theory and further researches in terms of photosynthesis and carbohydrate synthesis in response to elevated UV-B radiation.

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Effects of enhanced UV-B radiation (280–320 nm) on ribulose-1,5-bisphosphate carboxylase in pea and soybean

Cited by 82 publications

References 23 publications

Analysis of limitations to CO₂ assimilation on exposure of leaves of two Brassica napus cultivars to UV‐B

Analysis of limitations to CO₂ assimilation on exposure of leaves of two Brassica napus cultivars to UV‐B

Ultraviolet-Induced Photodegradation of Cucumber (Cucumis sativus L.) Microsomal and Soluble Protein Tryptophanyl Residues in Vitro

Changes in photosynthesis and carbohydrate synthesis in response to elevated UV-B environment

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Effects of enhanced UV-B radiation (280–320 nm) on ribulose-1,5-bisphosphate carboxylase in pea and soybean

Cited by 82 publications

References 23 publications

Analysis of limitations to CO2 assimilation on exposure of leaves of two Brassica napus cultivars to UV‐B

Analysis of limitations to CO2 assimilation on exposure of leaves of two Brassica napus cultivars to UV‐B

Ultraviolet-Induced Photodegradation of Cucumber (Cucumis sativus L.) Microsomal and Soluble Protein Tryptophanyl Residues in Vitro

Changes in photosynthesis and carbohydrate synthesis in response to elevated UV-B environment

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Analysis of limitations to CO₂ assimilation on exposure of leaves of two Brassica napus cultivars to UV‐B

Analysis of limitations to CO₂ assimilation on exposure of leaves of two Brassica napus cultivars to UV‐B