Effects of Epidermal Growth Factor, Platelet Derived Growth Factor and Growth Hormone 
 on Cultured Rat Keratinocytes Cells in vitro

Safari, Manouchehr; Ghahari, Laya; Zoroufchi, M D Babak Hossein Zadeh

doi:10.3923/pjbs.2014.931.936

Cited by 6 publications

(3 citation statements)

References 10 publications

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“…Since the total protein content increased proportionally to the amount of PL added during the fibrin assembly preparation process, the samples with higher concentrations of PL, i.e., the NF50 and NF100, could have been expected to contain a higher amount of each of the growth factor/bioactive compounds. The PDGF-BB at least was capable of participating in the significant beneficial effect of the PL in the NF50 and NF100 on the seeded HaCaT on these samples [30], together with other growth factors such as epidermal growth factor, which is usually present in PL [27]. The positive effect of the PL on the metabolic activity/viability of the HaCaT cells is illustrated in Figure 6.…”

Section: Discussionmentioning

confidence: 97%

The Effect of a Polyester Nanofibrous Membrane with a Fibrin-Platelet Lysate Coating on Keratinocytes and Endothelial Cells in a Co-Culture System

et al. 2021

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Chronic wounds affect millions of patients worldwide, and it is estimated that this number will increase steadily in the future due to population ageing. The research of new therapeutic approaches to wound healing includes the development of nanofibrous meshes and the use of platelet lysate (PL) to stimulate skin regeneration. This study considers a combination of a degradable electrospun nanofibrous blend of poly(L-lactide-co-ε-caprolactone) and poly(ε-caprolactone) (PLCL/PCL) membranes (NF) and fibrin loaded with various concentrations of PL aimed at the development of bioactive skin wound healing dressings. The cytocompatibility of the NF membranes, as well as the effect of PL, was evaluated in both monocultures and co-cultures of human keratinocytes and human endothelial cells. We determined that the keratinocytes were able to adhere on all the membranes, and their increased proliferation and differentiation was observed on the membranes that contained fibrin with at least 50% of PL (Fbg + PL) after 14 days. With respect to the co-culture experiments, the membranes with fibrin with 20% of PL were observed to enhance the metabolic activity of endothelial cells and their migration, and the proliferation and differentiation of keratinocytes. The results suggest that the newly developed NF combined with fibrin and PL, described in the study, provides a promising dressing for chronic wound healing purposes.

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Section: Discussionmentioning

confidence: 97%

The Effect of a Polyester Nanofibrous Membrane with a Fibrin-Platelet Lysate Coating on Keratinocytes and Endothelial Cells in a Co-Culture System

et al. 2021

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“…The normal skin development and function is influenced by hormones: for example, the growth hormone stimulates keratinocyte proliferation [ 39 ], and thyroid hormones act directly on hair follicles [ 40 ]. The skin is a neuroendocrine organ, capable of hormone synthesis and release [ 41 ]: corticosteroids and sex hormones are synthesized and transformed [ 42 ] (with sebocytes playing a central role in cutaneous androgen metabolism [ 43 ]); catecholamines are synthesized by keratinocytes and melanocytes [ 36 ].…”

Section: Introductionmentioning

confidence: 99%

The role of hormones in the pathogenesis of psoriasis vulgaris

Roman

Constantin

Marina

et al. 2015

Medicine and Pharmacy Reports

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Psoriasis vulgaris is a chronic, common skin disease, which affects the patient’s quality of life to the highest degree. Several exogenous factors and endogenous hormonal changes may act as triggers for psoriasis.The skin possesses a true endocrine system, which is very important in multiple systemic diseases. A number of conditions are associated with psoriasis, and its severity can also be influenced by hormones. Even though the sex hormones and prolactin have a major role in psoriasis pathogenicity, there are a lot of other hormones which can influence the psoriasis clinical manifestations: glucocorticoids, epinephrine, thyroid hormones, and insulin.

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“…The wound healing process can be improved by shortening the time needed for healing (5,6). Chemical and herbal medicines are locally or systemically administrated to help wound healing.…”

Section: Introductionmentioning

confidence: 99%

Healing Effects of Iranian Dwarf Elder on Full-thickness Epidermal Thermal Injury in Wistar Rats

haftador¹,

Ramhormozi²,

Yousefpour³

et al. 2022

Ann Mil Health Sci Res

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Background: Wound healing is a physiologic process that cells and tissues react when the skin is exposed to thermal injury. The inflammatory process has ‎essential effects on wound healing that includes coordination between immunological and biological responses. Chemical and herbal medicines are locally or systemically administrated to help wound healing. Herbal medicines have become more prevalent in recent years due to fewer side effects. Dwarf elder is used to treating inflammation and has been reported to possess antibacterial and antioxidant activities. This plant is also effective against burns, wounds, eczema, and infection. Objectives: In this study, we evaluated the topical application of dwarf elder on full-thickness epidermal thermal wounds in Wistar rats. Methods: Thirty adult male Wistar rats of about 300 g were used. The rats were randomly divided into three groups: Control group, vehicle group treated with an ointment base, and treatment group treated with elder ointment 10%. The animals were anesthetized and were created burn wounds with an area of 1 cm2 using the Meyer method. After 21 days, the wounds were photographed with a Canon camera. The wound area and healing degree were calculated. Slides were stained with H&E and examined by light microscopy and GraphPad Prism software. Data were analyzed by SPSS software using ANOVA and Tukey’s test. The significance level was considered at P < 0.05. Results: The wound area on the 21st day was significantly lower in the treatment group than in the control and vehicle groups. The epithelium thickness (μm) was significantly higher in the treatment group than in the control and vehicle groups. The blood vessels were significantly higher in the treatment group than in the other groups. Conclusions: The study showed that elder extract could be an effective remedy to repair and heal thermal injury and burn wounds.

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Effects of Epidermal Growth Factor, Platelet Derived Growth Factor and Growth Hormone on Cultured Rat Keratinocytes Cells in vitro

Cited by 6 publications

References 10 publications

The Effect of a Polyester Nanofibrous Membrane with a Fibrin-Platelet Lysate Coating on Keratinocytes and Endothelial Cells in a Co-Culture System

The Effect of a Polyester Nanofibrous Membrane with a Fibrin-Platelet Lysate Coating on Keratinocytes and Endothelial Cells in a Co-Culture System

The role of hormones in the pathogenesis of psoriasis vulgaris

Healing Effects of Iranian Dwarf Elder on Full-thickness Epidermal Thermal Injury in Wistar Rats

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