Effects of Equex from different sources on post-thaw survival, longevity and intracellular Ca2+ concentration of dog spermatozoa

Martínez, Ana Isabel Peña; Lugilde, Luz L; Barrio, M.; Herradón, P. G.; Arias, Luis Angel Quintela

doi:10.1016/s0093-691x(02)01233-5

Cited by 66 publications

(41 citation statements)

References 28 publications

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“…Equex STM, which Nöthling et al [7] and [9] added to the Triladyl, prolongs the maintenance of motility [15], [16], [17] and [18], membrane integrity [15] and [19] and acrosomal integrity [17], [20] and [19], calcium uptake [18] and fertility after intrauterine insemination [8] of frozen-thawed dog spermatozoa. Biladyl ® (Minitüb, GmbH, openUP introducing infectious agents into semen via the extender.…”

Section: Introductionmentioning

confidence: 99%

The effect of homologous prostatic fluid on motility and morphology of dog epididymal spermatozoa extended and frozen in Biladyl with Equex STM paste or Andromed

et al. 2007

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Although dog prostatic fluid decreases the longevity of ejaculated dog spermatozoa, it also increases their rate of motility and their fertility after vaginal insemination, as well as the fertility of epididymal spermatozoa after uterine insemination. These findings indicate a need to further characterize the effects of prostatic fluid on dog spermatozoa. This study was done to determine the effects (P < 0.05) of homologous prostatic fluid added prior to cooling, after thawing, or at both times to epididymal spermatozoa from 21 dogs. The

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Section: Introductionmentioning

confidence: 99%

The effect of homologous prostatic fluid on motility and morphology of dog epididymal spermatozoa extended and frozen in Biladyl with Equex STM paste or Andromed

et al. 2007

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“…As a result of freezing-thawing, the cell size decrease, especially the area and perimeter of the head [33][34][35][36]. Cryopreservation also affects the ultrastructure of spermatozoa [37][38][39][40]. Currently, common techniques are sperm cryopreservation in straws and in granules.…”

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confidence: 99%

Biological Integrity of Bison Epididymal Sperm Under Cryoconservation and Long Storage

Иолчиев¹,

Абилов²,

Таджиева³

et al. 2017

S-h. biol.

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A b s t r a c tConservation of biodiversity is one of the global challenges of the modern world. The preservation of animal genetic resources is considered essential for the food supply, since sustainable food production appears to be the greatest problem due to the human population growth, depletion of the Earth's natural resources, and many species becoming endangered. In situ and ex situ methods of preservation of the species (i.e. in/out of their natural habitats, respectively) are two major approaches to animal biodiversity conservation. Ex situ strategy involves the techniques for the genetic material cryopreservation. Cryopreservation of the wildlife biomaterials allows to use these genetic resources not only for the conservation and the renewal, but also for the introduction into the genotype of the farm animals. The bison (Bison bonasus) is identified as the rare and endangered species. At present, the free-living bison population in Russia comprises more than 1500 animals. A research concept of the Russian bison gene pool preservation includes creating cryo-preserved pool of bison spermatozoa. In this paper we report findings on biological adequacy of the cryopreserved epididymal bison semen after the long storage (for more than 20 years). The sperm samples were collected postmortem from the testicular appendages of four bison males sustained the injuries incompatible with life or culled and used for hunting. For the assessment of semen motility we used a computerassisted semen analysis (CASA) device; the DNA fragmentation index was assessed in AO-test with the acridine orange staining. The acrosomal integrity was studied by Diff-Quik staining method. It was shown that the semen quality parameters differed significantly due to the individual peculiarities of the bison. The spermatozoa of A + B grade which performed good motility and rectilinear motion reached more than 28 % in the semen of the males Mutfil and Morus, while in the Avel's and Misir's semen over 67 % spermatozoa were non-motile and 12.1 % and 10.4 % spermatozoa exhibited rotational and vibrational motions, respectively. The frequency of spermatozoa with pathomorphological changes significantly varied depending on the individual properties of the bison, with the greatest and the lowest values of 14.6 % and 6.8 %, respectively. The DNA fragmentation index reflecting sperm chromatin integrity can depend on the numerous biotic and abiotic factors and may vary in great ranges. In our surveys, it varied from 7 % to 86 %. For all the morphometric parameters, except the head width, the bison spermatozoa were inferior to the spermatozoa of the bulls though the differences between animal groups were not statistically significant. However, the area of the spermatozoa head in bulls was 3.14 m 2 larger than that of bison.

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“…In addition, we found that the sperm acrosome cap after thawing was protected by the addition of OEP to the extender [18]. Thus, OEP may be essential for preparing frozen canine semen [9,[11][12][13]15].The major ingredient of OEP is sodium lauryl (dodecyl) sulfate (SLS), but its concentration is unclear. Kato et al [7] employed a freezing extender of boar semen containing SLS (1.2 to 1.6 mg/ml) instead of OEP and reported that the percentage of normal acrosomes and progressively motile sperm after thawing with the addition of SLS were similar to that of OEP.…”

mentioning

confidence: 99%

mentioning

confidence: 99%

Effects of Addition of Sodium Lauryl Sulfate on Frozen-thawed Canine Spermatozoa

Hori

Fukuhara

et al. 2006

The Journal of Veterinary Medical Science

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ABSTRACT. The addition of Orvus ES paste (OEP) to extender may be essential for preparing frozen dog semen. The major ingredient of OEP is sodium lauryl sulfate (SLS). In this study, we compared the effect of SLS on frozen dog semen with that of OEP. There were no significant differences between the 2-mg/ml SLS group and OEP group concerning sperm motility, viability and the percentage of viable sperm with intact acrosomes after freeze-thawing. These results suggest that the effectiveness of frozen dog semen extender containing 2 mg/ml of SLS is similar effective to that demonstrated for OEP. KEY WORDS: canine, frozen semen, sodium lauryl sulfate.J. Vet. Med. Sci. 68(10): 1125-1128, 2006 Dog sperm is less freeze-tolerable than the sperm of other animals [5]. We previously reported that a freezing extender of canine semen, 20% (v/v) egg yolk tris-fructose citrate solution (EYT-FC) containing 0.75% Orvus ES paste (OEP) (Equex STM paste, Nova Chemical Sales, Scituate, MA, U.S.A.), a synthetic surfactant that is used in boars [6,14], increased sperm motility after thawing in comparison to extender without OEP addition and that sperm motility was maintained for a long period of time [17]. A high conception rate was achieved after artificial intrauterine insemination with the thawed semen [19]. In addition, we found that the sperm acrosome cap after thawing was protected by the addition of OEP to the extender [18]. Thus, OEP may be essential for preparing frozen canine semen [9,[11][12][13]15].The major ingredient of OEP is sodium lauryl (dodecyl) sulfate (SLS), but its concentration is unclear. Kato et al. [7] employed a freezing extender of boar semen containing SLS (1.2 to 1.6 mg/ml) instead of OEP and reported that the percentage of normal acrosomes and progressively motile sperm after thawing with the addition of SLS were similar to that of OEP. Furthermore, several studies have reported the usefulness of frozen semen extender containing SLS in many animals (the cow [3], mouse [4], goat [1,2], and boar [7]). In dogs, Peña et al. [10] added SLS to freezing extender for canine semen and reported that sperm viability after thawing was higher than for extender without SLS addition and that sperm survival was prolonged. However, no study has compared the usefulness of SLS and OEP added to freezing extender for canine semen.In this study, we investigated the optimal concentration of SLS supplemented to EYT-FC in a freezing method we previously reported [17] and compared the effect of SLS on the post-thaw survival of canine sperm with that of OEP.The male dogs used in this study, 6 beagles from 1 to 6 years of age, had normal semen quality and fertility. Two dogs were kept in each 160 × 75 × 65 cm cage. Commercial dog food (Hill's Canine Maintenance, Hill's-Colgate Ltd., Tokyo, Japan) was given once daily and drinking water was given three times daily. This study was conducted in conformity with the animal study guidelines of Nippon Veterinary and Life Science University.The semen samples, collected by digital manipul...

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Effects of Equex from different sources on post-thaw survival, longevity and intracellular Ca2+ concentration of dog spermatozoa

Cited by 66 publications

References 28 publications

The effect of homologous prostatic fluid on motility and morphology of dog epididymal spermatozoa extended and frozen in Biladyl with Equex STM paste or Andromed

The effect of homologous prostatic fluid on motility and morphology of dog epididymal spermatozoa extended and frozen in Biladyl with Equex STM paste or Andromed

Biological Integrity of Bison Epididymal Sperm Under Cryoconservation and Long Storage

Effects of Addition of Sodium Lauryl Sulfate on Frozen-thawed Canine Spermatozoa

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