Effects of Etch-and-Rinse and Self-etch Adhesives on Dentin MMP-2 and MMP-9

Mazzoni, Annalisa; Scaffa, Polliana Mendes Cándia; Carrilho, Marcela Rocha de Oliveira; Tjäderhane, Leo; Lenarda, Roberto Di; Polimeni, Antonella; Tezvergil‐Mutluay, Arzu; Tay, Franklin R.; Pashley, David H.; Breschi, Lorenzo

doi:10.1177/0022034512467034

Cited by 160 publications

(162 citation statements)

References 29 publications

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“…These results were more recently confirmed with specific assays of MMP-2 and MMP-9 in dentin matrices treated with both etch-and-rinse and self-etch adhesives Mazzoni, Scaffa, et al 2013). In addition, a self-etch adhesive has been shown to increase MMP-2 synthesis in human odontoblasts (Lehmann et al 2009), possibly increasing MMP-2 penetration into the HL via dentinal fluid.…”

Section: Role Of Mmps In Hl Degradationmentioning

confidence: 61%

Role of Dentin MMPs in Caries Progression and Bond Stability

et al. 2014

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Dentin can be described as a biological composite with collagen matrix embedded with nanosized hydroxyapatite mineral crystallites. Matrix metalloproteinases (MMPs) and cysteine cathepsins are families of endopeptidases. Enzymes of both families are present in dentin and collectively capable of degrading virtually all extracellular matrix components. This review describes these enzymes and their presence in dentin, mainly focusing on their role in dentin caries pathogenesis and loss of collagen in the adhesive hybrid layer under composite restorations. MMPs and cysteine cathepsins present in saliva, mineralized dentin, and/or dentinal fluid may affect the dentin caries process at the early phases of demineralization. Changes in collagen and noncollagenous protein structure may participate in observed decreases in mechanical properties of caries-affected dentin and reduce the ability of caries-affected dentin to remineralize. These endogenous enzymes also remain entrapped within the hybrid layer during the resin infiltration process, and the acidic bonding agents themselves (irrespective of whether they are etch-and-rinse or self-etch) can activate these endogenous protease proforms. Since resin impregnation is frequently incomplete, denuded collagen matrices associated with free water (which serves as a collagen cleavage reagent for these endogenous hydrolase enzymes) can be enzymatically disrupted, finally contributing to the degradation of the hybrid layer. There are multiple in vitro and in vivo reports showing that the longevity of the adhesive interface is increased when nonspecific enzyme-inhibiting strategies are used. Different chemicals (i.e., chlorhexidine, galardin, and benzalkonium chloride) or collagen cross-linker agents have been successfully employed as therapeutic primers in the bonding procedure. In addition, the incorporation of enzyme inhibitors (i.e., quaternary ammonium methacrylates) into the resin blends has been recently promoted. This review will describe MMP functions in caries and hybrid layer degradation and explore the potential therapeutic role of MMP inhibitors for the development of improved intervention strategies for MMP-related oral diseases.

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Section: Role Of Mmps In Hl Degradationmentioning

confidence: 61%

Role of Dentin MMPs in Caries Progression and Bond Stability

et al. 2014

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“…pH changes caused by acid etching or acidic monomers and the adhesive resin monomers themselves can modulate the activation and expression of MMPs and cysteine cathepsins, resulting in an increased digestion of collagen within the hybrid layer. 1,2 Different strategies have aimed to improve the bond durability by applying enzyme inhibitors as a pretreatment before resin infiltration or by admixing enzyme inhibitors to primers. It has been shown that chlorhexidine digluconate (CHX) can inhibit 3 MMP-2, À8, and À9 and of cysteine cathepsins, 4 preserving bond strength of etch-and-rinse and self-etching adhesives over time.…”

Section: Introductionmentioning

confidence: 99%

Effect of Different Matrix Metalloproteinase Inhibitors on Microtensile Bond Strength of an Etch-and-Rinse and a Self-etching Adhesive to Dentin

Pei

Zaruba

Attin

et al. 2015

Operative Dentistry

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Matrix metalloproteinase (MMP) inhibitors prevented bond strength loss of an etch-andrinse adhesive over time. Bond strength of a self-etching adhesive was not significantly reduced with 9 months of aging; thus, MMP inhibitors did not show a significant effect. SUMMARY Aim:This study aimed to analyze the effect of different matrix metalloproteinase (MMP) inhibitors on the microtensile bond strength (microTBS) of an etch-and-rinse and a selfetching adhesive after 9 months of aging.Methods and Materials: Flat human dentin surfaces were bonded either with an etch-andrinse adhesive (Optibond FL) or a self-etching adhesive (Clearfil SE Bond). Dentin surfaces were left untreated or were pretreated with MMP inhibitors (2% chlorhexidine digluconate [CHX], 0.05% green tea extract, 1 mM ferrous sulfate, or 0.2 mM galardin) prior to application of the adhesive. Composite buildups were made incrementally. Pretreated groups were tested after 9 months of storage in artificial saliva (378C) and compared with untreated groups, which were tested immediately (initial microTBS) and upon aging (9-month microTBS). Data were analyzed by linear mixedmodel regression. Failure mode analysis was performed microscopically and statistically analyzed by repeated-measures analysis of variance (p,0.05).

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“…However, when the dentin matrix mineralizes, MMPs become covered with apatite nanocrystals, making them immobile and nonfunctional. 5 As long as dentin is mineralized, its proteases remain structurally stable.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Hybrid Layer and Bonding Interface after Water Storage with and without the Usage of 2% Chlorhexidine: A Scanning Electron Microscope Study

Vallabhdas

Kumar

Kabbinale

et al. 2018

The Journal of Contemporary Dental Practice

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Aim:Restorative dentists employ different bonding systems between the resin and the dentin and other dentinal tissues to achieve the goal of micromechanical retention. Studies have shown that the bond between composite and dentin degrades over time because of the action of matrix metalloproteinases (MMPs) on collagen fibrils left unprotected by acid etching. The MMPs may be partially responsible for hybrid layer degradation. Since chlorhexidine (CHX) inhibits MMPs, we hypothesized that CHX would decelerate the loss of resin-dentin bonds. Hence, this in vitro study is intended to evaluate the effects of 2% CHX on hybrid layer and bonding interface. Materials and methods:Totally, 40 freshly extracted molars were randomly divided into four experimental groups. In all 40 specimens, class II cavities were prepared to a depth of 1 mm below the dentinoenamel junction with no axial wall, but the elimination of the proximal enamel ridge. The teeth were then randomly divided into four experimental groups, i.e., All Bond 2 without 2% CHX (group I), All Bond 2 with 2% CHX (group II), One Coat 7.0 without 2% CHX (group III), and One Coat 7.0 with 2% CHX (group IV). All the specimens were derooted and sectioned mesiodistally into two halves and placed under water at 37°C for 3 months and observed under scanning electron microscope for the hybrid layer and resin tag formation.Results: Groups I and II showed statistically significant difference when the presence/absence of resin tags was compared. Conclusion:Between all the four experimental groups, irrespective of the bonding systems used, we concluded that groups with 2% CHX usage showed promising results with presence/ absence of hybrid layer and resin tags formation.Clinical significance: Studies suggest that the bond between composite and dentin degrades over time because of the action of MMPs on collagen fibrils left unprotected by acid etching. Measures should be taken to prevent this from happening and thus allow bond between composite and dentin last longer.

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Effects of Etch-and-Rinse and Self-etch Adhesives on Dentin MMP-2 and MMP-9

Cited by 160 publications

References 29 publications

Role of Dentin MMPs in Caries Progression and Bond Stability

Role of Dentin MMPs in Caries Progression and Bond Stability

Effect of Different Matrix Metalloproteinase Inhibitors on Microtensile Bond Strength of an Etch-and-Rinse and a Self-etching Adhesive to Dentin

Evaluation of Hybrid Layer and Bonding Interface after Water Storage with and without the Usage of 2% Chlorhexidine: A Scanning Electron Microscope Study

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