Effects of Exercise on miR-29 and IGF-1 Expression and Lipid Profile in the Heart of Ovariectomized Rat

Habibi, Parisa; Alihemmatti, A.; Alipour, Mohammad Reza; Nourazar, Mir Alireza; Yousefi, Hadi; Andalib, Sasan; Ahmadiasl, Nasser

doi:10.4183/aeb.2016.130

Cited by 9 publications

(9 citation statements)

References 38 publications

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“…Cell apoptosis is well understood to play an important role in tissue homeostasis, such as in muscle myoblasts. As a negative regulator of myoblast apoptosis, miR-29 can affect the growth of rat myocardium by inhibiting IGF1 expression, 43 as well as inhibit proliferation and promote both apoptosis and differentiation of murine embryonic cardiomyocytes. 44 Herein, the co-transfection of goat primary myoblasts with si-circUSP13 and miR-29c inhibitor demonstrated that circUSP13 functions as a ceRNA of miR-29c regulating myoblast development.…”

Section: Discussionmentioning

confidence: 99%

Circular RNA circUSP13 sponges miR‐29c to promote differentiation and inhibit apoptosis of goat myoblasts by targeting IGF1

et al. 2021

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Circular RNAs (circRNAs) are an indispensable element of post‐transcriptional gene regulation, influencing a variety of biological processes including myogenic differentiation; however, little is known about the function of circRNA in goat myogenic differentiation. Using RNA‐sequencing data from our laboratory, we explored the influences of circUSP13, as a candidate circRNA, on myoblast differentiation since its expression is higher in myoblasts of lamb (first day of age) than that of the fetus (75th day of pregnancy). In in vitro experiments, circUSP13 significantly promoted differentiation and inhibited apoptosis in goat primary myoblasts. Mechanistically, circUSP13 localized with miR‐29c in the cytoplasm of goat myoblasts to regulate IGF1 expression. We further demonstrated that circUSP13 sponges miR‐29c, promoting IGF1 expression that upregulated the expression of MyoG and MyHC. Thus, our results identified circUSP13 as a molecular marker for breeding programs of mutton production, as well as the circUSP13‐miR‐29c‐IGF1 axis as a potential therapeutic target for combating muscle wasting.

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Section: Discussionmentioning

confidence: 99%

Circular RNA circUSP13 sponges miR‐29c to promote differentiation and inhibit apoptosis of goat myoblasts by targeting IGF1

et al. 2021

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“…Habibi et al . [46] recently reported the up-regulation of microRNA-29 with swimming training in OVX rats and suggested that swimming exercise training protectively regulates the heart against fibrosis by targeting the IGF-1 signalling pathway. These findings, combined with numerous reports on microRNA-29 gene as a fibrosis suppressor [15, 47], suggest that exercise training plays an important role in the development of cardiac fibrosis under pathological conditions.…”

Section: Discussionmentioning

confidence: 99%

Effects of six weeks of resistance-endurance training on microRNA-29 expression in the heart of ovariectomised rats

Jourkesh

Soori

Earnest

et al. 2018

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IntroductionHeart disease risk rises with age. However, women’s symptoms become more pronounced following the onset of menopause. The aim of the present study was to evaluate the effects of six weeks of combined resistance-endurance (RE) training on microRNA-29 expression in the heart of ovariectomised rats.Material and methodsThirty female Wistar rats were divided into three groups: 1) sham (SHAM); 2) ovariectomy (OVX); and 3) OVX with RE training (OVX + RE). The effects of these treatments on cardiac microRNA-29 expression were measured using real-time PCR. Data were analysed using a 2 × 3 ANOVA and Tukey post-hoc comparisons and presented as mean ±SEM.ResultsOvariectomy resulted in a significant down-regulation in the heart microRNA-29 gene expression of OVX (0.265 ±0.031 fold changes), OVX + RE (0.699 ±0.038 fold changes) in animals vs. sham animals (1 ±0 fold changes; all, p < 0.05) following six weeks of treatment. However, microRNA-29 expression in the OVX + RE group was significantly greater than in the OVX group (p < 0.05).ConclusionsOur findings suggest that the six weeks of regular RE training attenuate the reduction in heart muscle microRNA-29 expression observed in ovariectomised rates. If our findings carry over to humans, such an exercise regimen could be beneficial to the cardiovascular disease risk in women during menopause.

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“…The oviduct and each ovary were sectioned and separated. Then, a silk thread sutured the skin and muscle walls (24,25).…”

Section: Ovariectomy Surgerymentioning

confidence: 99%

The Combined Effect of High-intensity Interval Training and Soy Isoflavone Diet on MicroRNA-133 Gene in Ovariectomized Rats

Mirheidari

Soori

Ravasi

2022

J Kermanshah Univ Med Sci

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Background: The combination of exercise and soy extract supplementation decreases cardiac related disease risk in postmenopausal women. Objectives: We assessed the combined impacts of soy isoflavone (IF) supplementation and exercise on the expression of microRNA-133 gene in ovariectomized rats’ hearts. Methods: This study was conducted on 50 female Wistar rats, which were assigned to five groups, including sham-operated (SHAM), ovariectomized (OVX), ovariectomized with soy IF supplementation (OVX + IF), OVX with exercise (OVX + HIIT), and OVX with both soy IFs and exercise (OVX + IF + HIIT). The experimental rats underwent high-intensity interval training (5 days/week) and/or supplied a soy IF supplementation for six weeks. The cardiac microRNA-133 gene expression was analyzed using real-time PCR after six weeks of intervention. Results: Ovariectomy down-regulated cardiac microRNA133 gene expression, but 42 days of IF administration or HIIT reversed this effect (P > 0.05). The HIIT reversed estrogen-deficiency-related microRNA-133 down-regulation greater than IF or combining IF and HIIT (P > 0.05). The OVX + IF and OVX + IF + HIIT (P > 0.05) showed no significant difference. Conclusions: Our results suggested that treatment with exercise, soy IF, and the combined treatment could benefit microRNA-133 in ovariectomized rats. This effect was significant when exercise alone was used to induce treatment. Thus, these treatments could offer protection in the menopausal period.

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Effects of Exercise on miR-29 and IGF-1 Expression and Lipid Profile in the Heart of Ovariectomized Rat

Cited by 9 publications

References 38 publications

Circular RNA circUSP13 sponges miR‐29c to promote differentiation and inhibit apoptosis of goat myoblasts by targeting IGF1

Circular RNA circUSP13 sponges miR‐29c to promote differentiation and inhibit apoptosis of goat myoblasts by targeting IGF1

Effects of six weeks of resistance-endurance training on microRNA-29 expression in the heart of ovariectomised rats

The Combined Effect of High-intensity Interval Training and Soy Isoflavone Diet on MicroRNA-133 Gene in Ovariectomized Rats

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