Effects of Fe-deficient conditions on Fe uptake and utilization in P-efficient soybean

Abstract

Phosphorus (P)-efficient soybean (Glycine max) plants absorb and utilize P with high efficiency. To investigate the effects of iron (Fe)-deficient conditions on the absorption and utilization of Fe in P-efficient soybean plants, two soybean cultivars with different P efficiency, the 03-3 (P-efficient variety) and Bd-2 (P-inefficient variety), were used in this study. The two soybean cultivars were grown in nutrient solution containing Fe concentrations of 0 (Fe0), 20 (Fe20), 40 (Fe40), or 80 (Fe80) μM for 7 days. The Fe reductase activity of roots was higher in 03-3 plants grown under the Fe0, Fe20, and Fe40 treatments than in Bd-2 plants and the total Fe uptake was greater in 03-3 plants under the Fe40 treatment. GmFRD3a was much more highly expressed in the stem of 03-3 than in that of Bd-2, and significantly more iron was transported to 03-3 plant shoots during Fe0 treatment. Chlorosis in young leaves caused by Fe deficiency under the Fe0 and Fe20 treatments was alleviated by increased Fe concentration in shoots. Increased levels of active Fe in young 03-3 leaves under Fe-deprivation conditions (Fe0) and maintenance of stable Fe concentrations in 03-3 shoots subjected to Fe20, Fe40, and Fe80 treatments suggested that the P-efficient 03-3 cultivar is also Fe-efficient. It is suggested that 03-3 soybean cultivar should be a good resource for application to farm field.

A combined physiological and biophysical approach to understand the ligand‐dependent efficiency of 3‐hydroxy‐4‐pyridinone Fe‐chelates

“…We recognize that the lower value of root reductase activity obtained for plants grown without Fe supplementation can be misleading since plants in this condition are under severe stress and is not in agreement with studies is most plant species (Qiu et al, 2017). However, identical result has already been shown to happen in bean (Blair et al, 2010) and soybean (Santos et al, 2013), possibly due to the fact that the enzyme needs Fe for its functioning (Krishnan, 2005).…”

GsMATE encoding a multidrug and toxic compound extrusion transporter enhances aluminum tolerance in Arabidopsis thaliana

“…However, few studies have been conducted on MATE transporters in soybean. To date, only two MATE transporters, GmFRD3a and GmFRD3b, have been reported to play a role in iron homeostasis in soybean [ 31 , 55 ]. In the current study, GsMATE (accession number: BM732932.1) was cloned from the wild soybean root of the BW69 line (Al-resistant) of Glycine Soja , and the gene expression pattern was detected in response to Al treatment.…”

Foliar application of 3‐hydroxy‐4‐pyridinone Fe‐chelate [Fe(mpp) 3 ] induces responses at the root level amending iron deficiency chlorosis in soybean

“…Chlorophyll levels were lowest at the V3 leaf stage (between 35 and 40 days after planting depending on the Fe treatment, Figure 2), which is coherent with what was described in other studies with field‐grown soybean plants under Fe deficiency (Bai et al, 2018; Nenova, 2006). As shown after supplementation of other Fe‐chelates compounds (Bin et al, 2016; Ferreira et al, 2019; Martín‐Fernández, Solti, et al, 2017; Qiu et al, 2017), the pigment accumulation in leaves increased progressively, putatively reflecting the recovery of the photosynthetic apparatus, which was impaired due to Fe deficiency.…”