Effects of free antibiotic resistance genes in the environment on intestinal microecology of mice

Ding, Chengshi; Yang, Dong; Ma, Jing; Jin, Min; Shen, Zhiqiang; Shi, Danyang; Tian, Zhongjing; Kang, Meiling; Lǐ, Jùnwén; Qiu, Zhigang

doi:10.1016/j.ecoenv.2020.111119

Cited by 12 publications

(5 citation statements)

References 56 publications

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“…The highest ARG concentration detected in drinking water source samples varied between 4.18 and 7.66 log 10 copies/100 ml for different ARGs. Ding et al (2020) gavaged extracellular RK2 plasmid containing apramycin resistance gene at around 10 11 copies/ml concentration into mice and observed the bacterial transformation occurring in the proximal and distal jejuna and ilea for 2 days. After 2 days, transformants acted as the plasmid donors, and the RK2 plasmid was disseminated by conjugation for another 6 days before reaching a stable concentration ( Ding et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

“… Ding et al (2020) gavaged extracellular RK2 plasmid containing apramycin resistance gene at around 10 11 copies/ml concentration into mice and observed the bacterial transformation occurring in the proximal and distal jejuna and ilea for 2 days. After 2 days, transformants acted as the plasmid donors, and the RK2 plasmid was disseminated by conjugation for another 6 days before reaching a stable concentration ( Ding et al, 2020 ). The same scenario might occur in humans exposed to ARGs in drinking water which poses a human health risk.…”

Section: Discussionmentioning

confidence: 99%

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Prevalence of antibiotic resistance genes in drinking and environmental water sources of the Kathmandu Valley, Nepal

et al. 2022

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Antibiotic-resistant bacteria-associated infections are responsible for more than 1.2 million annual deaths worldwide. In low- and middle-income countries (LMICs), the consumption of antibiotics for human and veterinary uses is not regulated effectively. Overused and misused antibiotics can end up in aquatic environments, which may act as a conduit for antibiotic resistance dissemination. However, data on the prevalence of antibiotic resistance determinants in aquatic environments are still limited for LMICs. In this study, we evaluated the prevalence and concentration of antibiotic resistance genes (ARGs) in different drinking and environmental water sources collected from the Kathmandu Valley, Nepal, using droplet digital polymerase chain reaction to understand the current situation of ARG contamination. River water and shallow dug well water sources were the most contaminated with ARGs. Almost all samples contained sul1 (94%), and intI1 and tet(A) were detected in 83 and 60% of the samples, respectively. Maximum ARG concentration varied between 4.2 log10 copies/100 ml for mecA and 9.3 log10 copies/100 ml for sul1. Significant positive correlations were found between ARGs (r > 0.5, p < 0.01), except for mecA, qnrS, and vanA. As sul1 and intI1 were detected in almost all samples, the presence of these genes in a given sample may need to be considered as background antibiotic resistance in LMICs. Therefore, monitoring of ARGs, such as β-lactam ARGs, quinolone resistance genes, and vancomycin resistance genes, may provide a better picture of the antibiotic resistance determinants in aquatic environments of LMICs.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Prevalence of antibiotic resistance genes in drinking and environmental water sources of the Kathmandu Valley, Nepal

et al. 2022

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“…overgrowth after antibiotic treatment may play an important leading role in the microbiome or may be harmful to animals ( 43 ). Firmicutes and Proteobacteria flora are important carriers of antibiotic resistance, and we observed that the relative abundance of Escherichia , Staphylococcus , and Pseudomonas increased in antibiotic-treated mice ( 44 – 48 ). After fecal microbiota transplantation, the relative abundance of Streptococcus , Lachnoclostridium , Stenotrophomonas , Ochrobactrum , and Enterococcus maintained a consistent trend with that of giant pandas on day 3.…”

Section: Discussionmentioning

confidence: 72%

Dynamic Monitoring of Changes in Fecal Flora of Giant Pandas in Mice: Co-Occurrence Network Reconstruction

et al. 2023

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“…These results corroborate a previous study where the abundance of Tenericutes was higher in Poecilia reticulata gut growing in water bodies contaminated with antibiotic discharges from the nearby industries [ 26 ]. Tenericutes and its associated class, Mollicutes, are distinct taxonomic units that lack a cell wall that makes easier it for antibiotic resistance genes (ARGs) to be transformed into the bacterial cells [ 27 ]. Additionally, the abundance of Firmicutes has been observed in marketable fish that are treated with tetracycline since they possess tetA and tetO genes [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

Exposure to Oxy-Tetracycline Changes Gut Bacterial Community Composition in Rainbow Trout: A Preliminary Study

Choudhury

Park

Kim

et al. 2021

Animals

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The extensive use of antibiotics is evident in most of the livestock and aquaculture management for inhibiting pathogen infection. Korean aquaculture depends on the usage of oxy-tetracycline for growing rainbow trout. Hence, this study was conducted to evaluate the changes in gut bacterial community profiles of rainbow trout exposed to oxy-tetracycline and predict the metabolic functioning of the bacterial community. The gut bacterial community composition of oxy-tetracycline treated fish was assessed by amplicon sequencing targeting the 16S rRNA gene of bacteria and comparing with the control group that did not receive any antibiotic. The principle coordinate analysis and non-metric multidimensional scaling analysis had shown two distinct clusters that implies the changes in community composition. In phyla level, the relative abundances of Tenericutes and Firmicutes were observed to be significantly higher in oxy-tetracycline treated fish compared to the control. Furthermore, the prediction based metabolic profiling revealed the processes that are affected due to the shift in community profiles. For example, metabolic functioning of membrane efflux system, amino acid metabolism and glycolysis were significantly higher in oxy-tetracycline treated fish compared to the control. This study describes alteration in gut bacterial community composition and potential metabolic profiles of the community that might be responsible for surviving in antibiotic rich environment.

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Effects of free antibiotic resistance genes in the environment on intestinal microecology of mice

Cited by 12 publications

References 56 publications

Prevalence of antibiotic resistance genes in drinking and environmental water sources of the Kathmandu Valley, Nepal

Prevalence of antibiotic resistance genes in drinking and environmental water sources of the Kathmandu Valley, Nepal

Dynamic Monitoring of Changes in Fecal Flora of Giant Pandas in Mice: Co-Occurrence Network Reconstruction

Exposure to Oxy-Tetracycline Changes Gut Bacterial Community Composition in Rainbow Trout: A Preliminary Study

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