In this paper, for the first time, the effects of mixed neutron and gamma flux on the spectral and sensing responses of Long Period Gratings (LPGs) are thoroughly analyzed. Six LPGs written by means of Electric Arc Discharge (EAD) technique in standard and speciality fibers, including radiation-hardened ones, were tested. The EAD technique was chosen because it enables the writing of gratings both in standard and not photosensitive fibers. The experiments have been carried out in a “TRIGA” pulsed nuclear reactor and the LPGs were irradiated by a gamma-ray dose-rate of 9 Gy/s and a mean 1.2∙1012 n/(cm2s) neutron flux. Real time monitoring was performed for a comparative investigation of LPGs’ response, in terms of radiation sensitivity and wavelength shift. Experiments show that LPG in a radiation-resistant fiber exhibits resonant wavelength shift higher than LPG in standard fiber. The changes of temperature sensitivity due to radiation were experimentally established by comparison of pre- and post-radiation characterization, indicating that radiation effects induce a slight increase of the temperature sensitivity, except for the LPG in pure-silica fiber. Theoretical and numerical analysis was combined with experimental data for evaluation LPGs’ parameters changes, such as refractive index and thermo-optic coefficient, after exposure to radiation.