Effects of Gekko sulfated polysaccharide–protein complex on human hepatoma SMMC-7721 cells: Inhibition of proliferation and migration

Gekko-sulfated Glycopeptide Inhibits Tumor Angiogenesis by Targeting Basic Fibroblast Growth Factor

“…Reagents, Cell Lines, and Mice-GSPP was isolated and purified as described previously (25). The concentrations of GSPP used were 10, 100, and 200 g/ml in all cellular experiments unless indicated otherwise.…”

“…To evaluate the effects of GSPP on hepatoma cells, HepG2 and Bel-7402 cells were seeded at 2 ϫ 10 4 cells/well in 12-well plates, cultured for 72 h, and counted. Trypan blue exclusion, transwell, and wound-healing assays were performed as described previously (25).…”

“…The cells were cultured for 48 h and washed twice with PBS. Immunofluorescence staining of phalloidin rhodamine (Invitrogen) was performed as described previously (25).…”

“…We previously isolated a novel sulfated polypeptide, GSPP, from Gekko swinhonis Guenther and demonstrated its direct effects on the proliferation, differentiation, and migration of hepatoma cells (25)(26)(27). Here, we investigated the potent anti-angiogenic ability of GSPP in vitro and in vivo and found that bFGF signaling is involved in this process.…”

Sulfated polysaccharide-protein complex sensitizes doxorubicin-induced apoptosis of breast cancer cells in vitro and in vivo

“…Sulfated polysaccharide-protein complex (SPPC) is a cellular interstitial proteoglycan complex produced by L. japonica. Previous studies have demonstrated that SPPC has unique antitumor biological activities (15,16), and that numerous sulfated glycoproteins and sulfated polysaccharides are able to inhibit the proliferation and metastasis of tumor cells (17)(18)(19). In a study on the regulatory effect of sulfated polysaccharides on macrophage-induced tumor cell apoptosis, it was demonstrated that sulfated polysaccharides produced by L. japonica were able to indirectly enhance the cytotoxic effect of macrophages on HepG2 cells (16).…”