Effects of Genetic and Diet‐Induced Obesity on Lipid Metabolism

Abstract

SummaryC57BL/6J obese (ob/ob) and lean mice fed ad libitum on a normal mouse chow diet (Normal), were compared with lean mice of the same age and strain fed ad libitum on a high-fat diet, consisting of the Normal diet with the addition of beef lard (Lard), from age 3 months for 34 days. The lard-fed mice were seen to have significantly higher (P < < 0.05) body weight in this 34-day period than that of the other two groups fed on the Normal diet. Epididymal fat depot and adipocyte cell size were signi® cantly larger (P < < 0.05) in the Lard-fed lean mice and in the obese (ob/ob) mice than were those of the Normal-fed lean mice. Dietary Lard intake did not signi® cantly affect concentrations of plasma triglyceride although those of plasma cholesterol were signi® cantly increased (P < < 0.05). The development of obesity in these Lard-fed mice appeared to be accelerated and signi® cant. IUBMB Life, 48: 109± 113, 1999Keywords Diet-induced obesity; genetic obesity; high-saturated fat diet; mice. INTRODUCTIONExcess fat mass, characterising obesity, usually results from excessive energy storage over a prolonged period of time. This energy imbalance can theoretically result from excessive energy intake and lower energy expenditure, such as abnormal metabolic processes, impaired thermoregulation, or reduced physical activity. The development of obesity is believed to be in¯uenced by a number of factors, including genes and environment. Studies in animal models have clearly demonstrated that these 2 distinct types of obesity exist and that their mechanisms of excess fat mass accumulation may differ substantially (1). First is genetic obesity, as seen in rodent strains such as the Zucker fatty (fa/fa) rat and the obese (ob/ob) mouse used in this

A Comparative Study of Various Metabolic Parameters and Insulin Secretion in Overweight and Obese Individuals

“…Serum TG concentration in overweight and obese group having mean value, 143.6 mg/dL and 159.6 mg/dL respectively with P value 0.00001, which is towards higher side. This is similar to the work of Rokjan et al (14) Tissue resistance to insulin mediated glucose uptake which accelerate VLDL and TG production rate and decreased lipoprotein lipase mediated lipolysis in adipose tissue increases TG level.…”

“…Serum HDL concentration was decreased in both the groups, the mean value was 44 mg/dL and 38.4 mg/dL respectively which is similar to the work of various authors. (14,15) Serum total cholesterol and LDL concentration was also towards higher side in both groups which was 193.4 mg/dL and 222.43 mg/dL and 119.6 and 144.36 mg/dL respectively, with P value 0.0001 and 0.0001 respectively which is similar to the study of Golay et al (16,17) CONCLUSION: Overweight and obesity are global issues now, associated with various physiological abnormalities leading to diabetes mellitus and cardiovascular diseases. Present study is designed to study the effect of overweight and obesity on various parameters in coastal Andhra Pradesh and it was found that all the parameters in both the groups were towards higher side except few.…”

High-Fat Diet Promotes Neuronal Loss in the Myenteric Plexus of the Large Intestine in Mice

“…In mice, diet-induced obesity varies in intensity according to the type of diet and mouse strain used [22,23]. In the present study, the diet contained 31.2 % animal fat.…”

The mAb against adipocyte fatty acid-binding protein 2E4 attenuates the inflammation in the mouse model of high-fat diet-induced obesity via toll-like receptor 4 pathway

“…Therefore, as a "compensatory" response to 2E4 induced downregulation of A-FABP, E-FABP may be potentially upregulated, thus resulting in nonsignificant improvement on insulin tolerance; (5) mice with the diet-induced obesity do not develop the same degree of obesity or insulin resistance as genetically obese mice (Libinaki et al, 1999;Nilsson et al, 2012).…”