Effects of genipin cross-linking of chitosan hydrogels on cellular adhesion and viability

Gao, Lei; Gan, Hui; Meng, Zhiyun; Gu, Ruolan; Wu, Zhuona; Zhang, Ling; Zhu, Xiaoxia; Sun, Weizheng; Li, Jian; Zheng, Ying; Dou, Guifang

doi:10.1016/j.colsurfb.2014.03.002

Cited by 126 publications

(89 citation statements)

References 36 publications

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“…There were no significant differences ( > 0.05) among contact angles of gels (b) and (c), indicating that the additional increase of genipin amount above 3.5% w/w dry chitosan did not markedly affect the hydrophilicity of the gel surface. These results are in accordance with the study performed by Gao et al [32]. Figure 5 shows FTIR spectra recorded for pellets of genipin, a CS control, and CS-genipin complex.…”

Section: Contact Angle Analysis Contact Angles Of Different Hydrogelsupporting

confidence: 81%

“…Basic biocompatibility of the GN1-and GN2-type structures was preliminarily evaluated by assaying proliferation of cultured human-derived cell lines after 1 and 7 days of culture, chosen as time points for assessing the short-term proliferative behavior of cells. To this aim, the immortalized MG63 human osteoblast-like cells and human mesenchymal stem cells (hMSCs) were adopted as assessed models for bone tissue engineering [32,33,38]. Results from MTT assays on MG63 seeded onto GN1 and GN2 scaffolds showed no significant effects on metabolic activity and proliferation of cells with respect to the control cells grown on standard culture plates.…”

Section: Biocompatibility Evaluation Assays On Human-derivedmentioning

confidence: 99%

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Effects of Genipin Concentration on Cross-Linked Chitosan Scaffolds for Bone Tissue Engineering: Structural Characterization and Evidence of Biocompatibility Features

Dimida

Barca

Cancelli

et al. 2017

International Journal of Polymer Science

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Genipin (GN) is a natural molecule extracted from the fruit of Gardenia jasminoides Ellis according to modern microbiological processes. Genipin is considered as a favorable cross-linking agent due to its low cytotoxicity compared to widely used cross-linkers; it cross-links compounds with primary amine groups such as proteins, collagen, and chitosan. Chitosan is a biocompatible polymer that is currently studied in bone tissue engineering for its capacity to promote growth and mineral-rich matrix deposition by osteoblasts in culture. In this work, two genipin cross-linked chitosan scaffolds for bone repair and regeneration were prepared with different GN concentrations, and their chemical, physical, and biological properties were explored. Scanning electron microscopy and mechanical tests revealed that nonremarkable changes in morphology, porosity, and mechanical strength of scaffolds are induced by increasing the cross-linking degree. Also, the degradation rate was shown to decrease while increasing the crosslinking degree, with the high cross-linking density of the scaffold disabling the hydrolysis activity. Finally, basic biocompatibility was investigated in vitro, by evaluating proliferation of two human-derived cell lines, namely, the MG63 (human immortalized osteosarcoma) and the hMSCs (human mesenchymal stem cells), as suitable cell models for bone tissue engineering applications of biomaterials.

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Section: Contact Angle Analysis Contact Angles Of Different Hydrogelsupporting

confidence: 81%

Section: Biocompatibility Evaluation Assays On Human-derivedmentioning

confidence: 99%

Effects of Genipin Concentration on Cross-Linked Chitosan Scaffolds for Bone Tissue Engineering: Structural Characterization and Evidence of Biocompatibility Features

Dimida

Barca

Cancelli

et al. 2017

International Journal of Polymer Science

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“…It can crosslink macromolecules such as polysaccharides and proteins and is a well-known crosslinker of chitosan, a natural polymer consisting of many amino groups. The degree of crosslinking by genipin is mainly dependent on the availability of free amino groups to which it can react under mild conditions and can clearly be noted by the blue color change that occurs upon crosslinking (29,30). In comparison to other potent crosslinkers as mentioned above, genipin can produce crosslinked materials with comparative degradative and mechanical properties (30).…”

Section: Introductionmentioning

confidence: 99%

“…The degree of crosslinking by genipin is mainly dependent on the availability of free amino groups to which it can react under mild conditions and can clearly be noted by the blue color change that occurs upon crosslinking (29,30). In comparison to other potent crosslinkers as mentioned above, genipin can produce crosslinked materials with comparative degradative and mechanical properties (30). Within the field of biotechnology and biomaterials, Genipin is widely employed in studies related to tissue engineering, regeneration, and fixation.…”

Section: Introductionmentioning

confidence: 99%

“…In addition, it has been used in the formation of interpenetrating polymer networks (IPN), nanocomposites, nanoparticles, as well as microspheres and macrogels (29). Of particular interest in this study is its use as a natural crosslinker to form a semi-interpenetrating polymer network (s-IPN) whereby it also provides further advantageous therapeutic benefits relating to wound healing such as anti-inflammatory properties (30).…”

Section: Introductionmentioning

confidence: 99%

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Synthesis of a Semi-Interpenetrating Polymer Network as a Bioactive Curcumin Film

et al. 2014

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Abstract. This study focused on the synthesis and characterization of a natural polymeric system employing the interpenetrating polymer network (IPN) comprising curcumin as a bioactive. Biopolymers and actives such as chitosan, hypromellose, citric acid, genipin, and curcumin were used to develop an effective, biodegradable, and biocompatible film employed therapeutically as a wound healing platform. The semi-IPN films were investigated for their physicochemical, physicomechanical, and biological properties by quantification by FTIR, DSC, and Young's modulus. Following characterization, an optimum candidate formulation was produced whereby further in vitro and ex vivo studies were performed. Results revealed a burst release occurring at the first hour with 1.1 mg bioactive released when in contact with the dissolution medium and 2.23 mg due to bioactive permeation through the skin, thus suggesting that the lipophilic nature of skin greatly impacted the bioactive release rate. Furthermore, chemical and mechanical characterization and tensile strength analysis revealed that the degree of crosslinking and concentration of polymeric material used significantly influenced the properties of the film.

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Biodegradable, Sustainable Hydrogel Actuators with Shape and Stiffness Morphing Capabilities via Embedded 3D Printing

Sun

Williamson

Sukhnandan

et al. 2023

Adv Funct Materials

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Despite the impressive performance of recent marine robots, many of their components are non‐biodegradable or even toxic and may negatively impact sensitive ecosystems. To overcome these limitations, biologically‐sourced hydrogels are a candidate material for marine robotics. Recent advances in embedded 3D printing have expanded the design freedom of hydrogel additive manufacturing. However, 3D printing small‐scale hydrogel‐based actuators remains challenging. In this study, Free form reversible embedding of suspended hydrogels (FRESH) printing is applied to fabricate small‐scale biologically‐derived, marine‐sourced hydraulic actuators by printing thin‐wall structures that are water‐tight and pressurizable. Calcium‐alginate hydrogels are used, a sustainable biomaterial sourced from brown seaweed. This process allows actuators to have complex shapes and internal cavities that are difficult to achieve with traditional fabrication techniques. Furthermore, it demonstrates that fabricated components are biodegradable, safely edible, and digestible by marine organisms. Finally, a reversible chelation‐crosslinking mechanism is implemented to dynamically modify alginate actuators' structural stiffness and morphology. This study expands the possible design space for biodegradable marine robots by improving the manufacturability of complex soft devices using biologically‐sourced materials.

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Effects of genipin cross-linking of chitosan hydrogels on cellular adhesion and viability

Cited by 126 publications

References 36 publications

Effects of Genipin Concentration on Cross-Linked Chitosan Scaffolds for Bone Tissue Engineering: Structural Characterization and Evidence of Biocompatibility Features

Effects of Genipin Concentration on Cross-Linked Chitosan Scaffolds for Bone Tissue Engineering: Structural Characterization and Evidence of Biocompatibility Features

Synthesis of a Semi-Interpenetrating Polymer Network as a Bioactive Curcumin Film

Biodegradable, Sustainable Hydrogel Actuators with Shape and Stiffness Morphing Capabilities via Embedded 3D Printing

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