Effects of geroprotective peptides on the activity of cholinesterases and formation of the soluble form of the amyloid precursor protein in human neuroblastoma SH-SY5Y cells

Nalivaeva, Natalia N.; Makova, Natalia Z.; Kochkina, E. G.; John, Danielle; Arutyunov, V. A.; Kozina, L S; Arutjunyan, А. V.; Журавин, И. А.

doi:10.1134/s1819712411030044

Cited by 4 publications

(2 citation statements)

References 30 publications

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“…The supernatant was used for the enzymatic acetylcholinesterase (AChE) analyses. AChE activity was determined according to the Ellman method [ 22 ], with some modifications [ 23 ]. Hydrolysis rates were measured at acetylthiocholine concentration of 9.6 mM in 130 μL assay solution with 200 mM Na-phosphate buffer (pH 7.5) and 0.39 mM 5,5′-dithiobis-(2-nitrobenzoic acid) (DTNB).…”

Section: Methodsmentioning

confidence: 99%

Prenatal Hyperhomocysteinemia Induces Glial Activation and Alters Neuroinflammatory Marker Expression in Infant Rat Hippocampus

Shcherbitskaia

Vasilev

Milyutina

et al. 2021

Cells

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Maternal hyperhomocysteinemia is one of the common complications of pregnancy that causes offspring cognitive deficits during postnatal development. In this study, we investigated the effect of prenatal hyperhomocysteinemia (PHHC) on inflammatory, glial activation, and neuronal cell death markers in the hippocampus of infant rats. Female Wistar rats received L-methionine (0.6 g/kg b.w.) by oral administration during pregnancy. On postnatal days 5 and 20, the offspring’s hippocampus was removed to perform histological and biochemical studies. After PHHC, the offspring exhibited increased brain interleukin-1β and interleukin-6 levels and glial activation, as well as reduced anti-inflammatory interleukin-10 level in the hippocampus. Additionally, the activity of acetylcholinesterase was increased in the hippocampus of the pups. Exposure to PHHC also resulted in the reduced number of neurons and disrupted neuronal ultrastructure. At the same time, no changes in the content and activity of caspase-3 were found in the hippocampus of the pups. In conclusion, our findings support the hypothesis that neuroinflammation and glial activation could be involved in altering the hippocampus cellular composition following PHHC, and these alterations could be associated with cognitive disorders later in life.

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Section: Methodsmentioning

confidence: 99%

Prenatal Hyperhomocysteinemia Induces Glial Activation and Alters Neuroinflammatory Marker Expression in Infant Rat Hippocampus

Shcherbitskaia

Vasilev

Milyutina

et al. 2021

Cells

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“…AChE and BChE activities were assayed by our modification of Ellman's procedure in 96-well plates as described before [30] in the incubation medium containing: 0.025 ml of 0.002 М dithionitrobensoic acid (5,5'-dithio-bis-(2-nitrobenzoic acid)-DTNB), dissolved in 200 mМ Naphosphate buffer (рН 7.5), 0.0125 ml of 0.01 М reaction substrates either acetylthiocholine iodide (ATCh) or butyrylthiocholine iodide (BTCh) in water, 20-50 μl of protein fractions, containing the analysed enzymes, and bi-distilled Н 2 О up to the volume of 0.125 ml. The reaction was initiated by addition of the substrate.…”

Section: Methodsmentioning

confidence: 99%

Effect of hypoxia on cholinesterase activity in rat sensorimotor cortex

Kochkina

Plesneva

Журавин

et al. 2015

J Evol Biochem Phys

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This study reports the dynamics of changes in postnatal ontogenesis of the activity of soluble and membrane-bound forms of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) in the sensorimotor cortex of rats as well as the character of their changes after prenatal hypoxia (Е14, 7% О 2 , 3 hours) or acute hypoxia in adult animals (4 months, 7% О 2 , 3 hours). In normally developing rats the activity of the membrane-bound form of AChE in the sensorimotor cortex gradually increased up to the end of the first month after birth and remained at this high level during all further postnatal ontogenesis while the activity of the soluble form of the enzyme reached its maximum value on the 10th day after birth and decreased significantly by the end of the first month. In animals subjected to prenatal hypoxia the activity both of the soluble and membrane bound forms of AChE during the first two weeks after birth was 20-25% lower compared to controls but increased by the end of the first month and even exceeded the control values and remained increased up to old age (1.5 years). The activity of both forms of BChE in rat sensorimotor cortex at all stages of postnatal ontogenesis was significantly lower than of AChE although the dynamics of their changes was similar to AChE. Prenatal hypoxia led to a decrease in the activity of the membrane-bound form of BChE compared to controls practically at all studied stages of development but was higher at the end of the first month after birth. At the same time, the activity of the soluble form of BChE was decreased only on the 20th day of development compared to the control but increased starting from the end of the first month of life and further. Acute hypoxia in adult rats also led to a decrease in the activity of both forms of AChE and BChE in the sensorimotor cortex but the dynamics of these changes was different. Thus, insufficient oxygen supply to the nervous tissue at different stages of ontogenesis has a significant effect on the activity and ratio of various forms of cholinesterases possessing either growth factor or mediator properties which might lead to the changes in brain development and formation of behavioural reactions including learning and memory аs well as increase the risk of development of the sporadic form of Alzheimer's disease (AD)-one of the most common neurodegenerative diseases of advanced age. This study widening our understanding of the properties of brain cholinesterases under normal and pathological conditions might be useful for developing new approaches towards prevention and treatment of AD.

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Role of caspase-3 in regulation of the amyloid-degrading neuropeptidase neprilysin level in the rat cortex after hypoxia

Козлова¹,

Vasylev²,

Dubrovskaya³

et al. 2015

J Evol Biochem Phys

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Effects of geroprotective peptides on the activity of cholinesterases and formation of the soluble form of the amyloid precursor protein in human neuroblastoma SH-SY5Y cells

Cited by 4 publications

References 30 publications

Prenatal Hyperhomocysteinemia Induces Glial Activation and Alters Neuroinflammatory Marker Expression in Infant Rat Hippocampus

Prenatal Hyperhomocysteinemia Induces Glial Activation and Alters Neuroinflammatory Marker Expression in Infant Rat Hippocampus

Effect of hypoxia on cholinesterase activity in rat sensorimotor cortex

Role of caspase-3 in regulation of the amyloid-degrading neuropeptidase neprilysin level in the rat cortex after hypoxia

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