Effects of glucose on the decolorization of Reactive Black 5 by yeast isolates

Abstract

The cometabolic roles of glucose were investigated in decolorization of an azo dye, Reactive Black 5, by yeast isolates, Debaryomyces polymorphus and Candida tropicalis. The results indicated that the dye degradation by the two yeasts was highly associated with the yeast growth process and glucose presence in the medium. Color removal of 200 mg dye/L was increased from 76.4% to 92.7% within 60 h to 100% within 18-24 h with the increase of glucose from 5 to 10 g/L, although the activity of manganese dependent peroxidase (MnP) decreased by 2-8 times in this case. Hydrogen peroxide of 233.3 µg/L was detected in 6 h in D. polymorphus culture. The cometabolic functions of glucose and hydrogen peroxide could be also confirmed by the further color removals of 95.8% or 78.9% in the second cycle of decolorization tests in which 7 g glucose/L or 250 µg H 2 O 2 /L was superadded respectively together with 200 mg dye/L.

Optimization of culture medium composition for manganese peroxidase and tyrosinase production during Reactive Black 5 decolourization by the yeast Trichosporon akiyoshidainum

“…Besides, yeasts have been found to be very efficient in treating high-strength organic wastewaters (Yang et al, 2008). Ascomycetous yeasts are able to decolourize reactive dyes by biodegradation; reduction of azo-bond, biosorption or catalytic degradation by ligninolytic enzymes.…”

Aerobic decolorization of azo dye orange g by a new yeast isolate Candida cylindracea SJL6

“…Thus, the color removal increased from 76.4% and 92.7%, respectively, after 60 h to 100% between 18 h and 24 h after rising glucose 1344 Aerobic decolorization... ALCÂNTARA, T. A. P. et al concentration from 0.5% to 1% (YANG et al, 2008). The optimal glucose concentration was reported to be between 0.5 and 1% for several yeasts such as Pichia sp.…”

“…Although there are reports of yeasts that can use a dye as a sole source of carbon and/or nitrogen for dye degradation, this process is highly associated with the cell growth and primary metabolism (LUCAS et al, 2006;YANG et al, 2008;PAJOT et al, 2011;MORTARELL et al, 2012). Therefore, yeasts do not generally grow without glucose or any other readily metabolizable carbon source (SOLÍS et al, 2012).…”

“…The use of yeasts presents several biotechnological advantages, not only because of their rapid growth, but also because of their resistance to unfavorable environments and high efficiency in azo dye decolorization (MORTARELL et al, 2012;YANG et al, 2008;LIU et al, 2011;PAJOT et al, 2011;TAN et al, 2014).…”

Use of response surface methodology for the bioaccumulation of Violet 90 metal-complex dye by Candida tropicalis

“…However, little work has been carried out investigating the ability of yeast to act as a bioaccumulator for textile dyes in wastewater effluents [6,7]. Candida oleophila [22], Candida zeylanoides [23,24], Candida tropicalis and Debaryomyces polymorphus [17,25], Kluyveromyces marxianus IMB3 [18], Candida tropicalis [4,7,16,26], Saccharomyces cerevisiae [6], Candida utilis [27], Trichosporon beigelii NCIM-3326 [28], Rhodotorula mucilaginosa [8], and Pichia fermentans [29] yeasts have been used for the removal of different dyes. On the other hand, even though metal-complex dyestuffs are frequently utilized in the textile industry for coloring wool, nylon, cotton, and silk, studies about their bioaccumulation with microorganisms from waste waters are very few.…”