Effects of growth conditions on exopolysaccharide production by Cyanospira capsulata

Philippis, Roberto De; Chen, Sili; Tassinato, Graziano; Vincenzini, Massimo; Materassi, R.

doi:10.1016/0960-8524(91)90138-a

Cited by 57 publications

(34 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…LX1, respectively. Cyanothece 16 SOM 2 increases EPS release under P-starvation (De Philippis et al 1993 ), while in the case of Phormidium J-1 and Cyanospira capsulata no signifi cant effects were observed (De Philippis et al 1991 ;Fattom and Shilo 1984 ). For Anabaena cylindrica 10 °C, a decrease in EPS release was observed under P-shortage (Lama et al 1996 ).…”

Section: Phosphorus Availabilitymentioning

confidence: 50%

“…Studying Arthrospira platensis , they quantifi ed the concentration at which carbohydrates and biomass production is maximum, which corresponds to 1.82 ± 0.16 mg P g −1 dry biomass. The most frequently observed effect of P-starvation is the switch from protein synthesis to carbohydrate and lipid accu-mulation (Markou et al 2012 ), although no carbohydrate accumulation was observed in some cases (De Philippis et al 1991 ;Ji et al 2011 ) and a decrease in carbohydrate content was observed in others (Nicolaus et al 1999 ). Rodolfi et al ( 2009 ) and Xin et al ( 2010 ) reported only lipid accumulation for Nannochloropsis sp.…”

Section: Phosphorus Availabilitymentioning

confidence: 92%

See 1 more Smart Citation

Exocellular Polysaccharides in Microalgae and Cyanobacteria: Chemical Features, Role and Enzymes and Genes Involved in Their Biosynthesis

Rossi

Philippis

2016

The Physiology of Microalgae

View full text Add to dashboard Cite

Aims and ScopeApplied Phycology, the practical use of algae, encompasses a diverse range of fields including algal culture and seaweed farming, the use of algae to produce commercial products such as hydrocolloids, carotenoids and pharmaceuticals, algae as biofertilizers and soil conditioners, the application of algae in wastewater treatment, renewable energy production, algae as environmental indicators, environmental bioremediation and the management of algal blooms. The commercial production of seaweeds and microalgae and products derived there from is a large and well established industry and new algal species, products and processes are being continuously developed.The aim of this book series, Developments in Applied Phycology, is to present state-ofthe-art syntheses of research and development in the field. Volumes of the series will consist of reference books, subject-specific monographs, peer reviewed contributions from conferences, comprehensive evaluations of large-scale projects, and other booklength contributions to the science and practice of applied phycology.

show abstract

Section: Phosphorus Availabilitymentioning

confidence: 50%

Section: Phosphorus Availabilitymentioning

confidence: 92%

Exocellular Polysaccharides in Microalgae and Cyanobacteria: Chemical Features, Role and Enzymes and Genes Involved in Their Biosynthesis

Rossi

Philippis

2016

The Physiology of Microalgae

View full text Add to dashboard Cite

show abstract

“…We expect the activity of microbial flora surrounding or embedded in colonies to be stimulated by C. watsonii's ex creted products, which might create hypoxic conditions during the O 2 -sensitive N 2 fixation process. In addition, EPS excretion is also invoked as a possible chelator for micronutrients such as iron or calcium (De Philippis et al 1991), which are both essential to diazotrophs for nitrogen fixation. Lastly, it has been hypothesized that EPS could also pro tect nitrogenase against harmful effects of oxygen in the cyanobacterium Nostoc cordubensis (Prosperi 1994).…”

Section: Overproduction Of Organic Carbon Bymentioning

confidence: 99%

Light:dark (12:12 h) quantification of carbohydrate fluxes in Crocosphaera watsonii

Dron¹,

Rabouille²,

Claquin³

et al. 2012

Aquat. Microb. Ecol.

View full text Add to dashboard Cite

Light:dark (12:12 h) quantification of cellular carbohydrate fluxes was conducted with the unicellular, N 2 -fixing cyanobacterium Crocosphaera watsonii WH8501 grown in continuous cultures under conditions of diazotrophy. As commonly observed in photoautotrophs, C. watsonii accumulates photosynthetic products as carbohydrates, which thereafter serve as an internal energy reserve for energy-demanding processes. Our goal was to further discriminate between cellular pools with different sizes to observe their respective dynamics. Total cellular carbohydrate as well as the cellular carbohydrate pools showed a diel cycle of accumulation and decrease. Total cellular carbohydrate contents represented 45 and 32% of the cellular carbon content at the end of the light and dark period, respectively. The observed decrease in cellular carbohydrate was not only due to carbohydrate catabolism but also to their release in the environment as extracellular polymeric substances (EPS), including soluble EPS and transparent exopolymeric particles (TEP). The occurrence of such excretion during the exponential growth of C. watsonii appears as part of the metabolic regulations that participate in maintaining a daily carbon:nitrogen balanced growth. About 10% of the total carbon cell content is excreted daily as soluble EPS. TEP, which are formed from dissolved precursors (including some soluble EPS) released by C. watsonii, increased during the dark period, when they represented up to 3.6% of total carbon cell content. Such recurrent release of carbohydrate by populations of C. watsonii is expected to stimulate the microbial loop in warm, oligotrophic oceans.

show abstract

“…These biopolymers have been attracting interest due to their great application potential in food, cosmetic, pharmaceutical and oil industries, where they are used as thickening, stabilizing and emulsifying agents. Therefore, the identification of new production sources for these materials is of great interest, specially when such biopolymers possess good rheological properties (4).…”

Section: Introductionmentioning

confidence: 99%

Optimization of polysaccharides production by bacteria isolated from soil

Bueno¹,

García-Cruz²

2006

Braz. J. Microbiol.

View full text Add to dashboard Cite

Six polysaccharide-producing bacteria, isolated from soil samples and identified as Pseudomonas and Arthrobacter (Strains 3B, 4B, 7B, 21B, 18E and 21D), were tested for the yield of polysaccharides produced during growth in two culture media: one containing glucose and the other sucrose (1, 2, 3, 4 and 5%). The yield was quantified measuring the viscosity of the broth, using the Poiscuille equation. The effect of temperature and pH of the culture media was investigated. The largest polysaccharide yield was obtained when the concentration of the carbon source was lower than 2%. Glucose and sucrose stimulated the polysaccharide production in a similar way. When the initial pH of the fermentation broth was increased from 5.0 up to 7.0, there was an increase in polysaccharide production. However, higher values (pH=8.0) caused a decrease in polysaccharide production. With regard to temperature, 30ºC was shown to be optimum, since higher or lower temperatures had a negative effect on saccharide production.

show abstract

Effects of growth conditions on exopolysaccharide production by Cyanospira capsulata

Cited by 57 publications

References 11 publications

Exocellular Polysaccharides in Microalgae and Cyanobacteria: Chemical Features, Role and Enzymes and Genes Involved in Their Biosynthesis

Exocellular Polysaccharides in Microalgae and Cyanobacteria: Chemical Features, Role and Enzymes and Genes Involved in Their Biosynthesis

Light:dark (12:12 h) quantification of carbohydrate fluxes in Crocosphaera watsonii

Optimization of polysaccharides production by bacteria isolated from soil

Contact Info

Product

Resources

About