Effects of growth regulators and genotypes on pyrethrum In vitro

Obukosia, Silas D.; Kimani, Esther; Waithaka, K.; Mutitu, Eunice; Kimani, Paul M.

doi:10.1079/ivp2004620

Cited by 12 publications

(9 citation statements)

References 10 publications

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“…1.0 mgl -1 NAA. These results are similar to those reported by Obukosia et al (2005), who found that a high 2,4-D concentration resulted in reduced callus formation and the growth of pyrethrum. Calluses induced by 1.0 mg l -1 2,4-D ?…”

Section: Establishment Of Callus Inductionsupporting

confidence: 92%

Micropropagation of bromeliad Aechmea fasciata via floral organ segments and effects of acclimatization on plantlet growth

Huang

Liao

Tsai

et al. 2010

Plant Cell Tiss Organ Cult

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Although suckers and seedlings can be used for the propagation of bromeliads, the low number of propagules and cross-variation limit their uniformity and mass cultivation. In this study, high-efficiency shoot organogenesis and plant regeneration were achieved on callus derived from petal and ovary explants of Aechmea fasciata (Bromeliaceae). Calluses were induced on half-strength Murashige and Skoog inorganic salts (1/2MS) supplemented with 1.0-1.5 mg l -1 2,4-dichlorophenoxyacetic acid in combination with 1.0 or 0.5 mg l -1 a-naphthaleneacetic acid (NAA), and shoots regenerated after transfer to 1/2MS basal medium containing the combination of 1.0 mg l -1 NAA ? 0.5 mg l -1 1-phenyl-3-(1,2,3-thiadiazol-5-yl) urea. Those plantlets grown under a middle light intensity (50 lmol m -2 s -1 ) showed a dramatic increase in survival percentage (up to 95%) and the maximum number of newly developing roots. The plantlets that were transplanted onto pots were successfully grown in the greenhouse.

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Section: Establishment Of Callus Inductionsupporting

confidence: 92%

Micropropagation of bromeliad Aechmea fasciata via floral organ segments and effects of acclimatization on plantlet growth

Huang

Liao

Tsai

et al. 2010

Plant Cell Tiss Organ Cult

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“…Although 2,4-D is a phenoxyalkanoic herbicide, it acts as an auxin in inducing cell division and enlargement at low concentrations. Cell enlargement has been associated with an increase in the activities of autolytic and synthetic enzymes, which affect cell wall plasticity and synthesis of new cell wall materials (Obukosia et al 2005). Sakuta et al (1991) reported that 2,4-D (5 μM) strongly promoted betacyanin accumulation in suspension cultures Lowercase letters (a, b) denote significant differences (one-way ANOVA, p=0.05) according to Duncan's multiple range test.…”

Section: Discussionmentioning

confidence: 99%

Growth regulator-induced betacyanin accumulation and dopa-4,5-dioxygenase (DODA) gene expression in euhalophyte Suaeda salsa calli

Zhao

Sun

Gao

et al. 2011

In Vitro Cell.Dev.Biol.-Plant

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Suaeda salsa calluses cultured in darkness for 28 d were transferred to Murashige and Skoog (MS) media containing various growth regulators under white light conditions for 10 d to investigate cell growth, betacyanin accumulation, and expression of dopa-4,5-dioxygenase (DODA). Callus growth was markedly promoted when 0.2 mg·L −1 2,4-D and 0.5 mg·L −1 6-BA were added to the MS medium. Surprisingly, of the auxins tested, IAA had no effect on betacyanin content, but 2,4-D strongly decreased betacyanin content. Betacyanin content was positively correlated with 6-BA concentrations in the range of 0.1-2.0 mg·L −1 . DODA mRNA levels were consistent with the response of betacyanin content to exogenous growth regulators. These results suggest that betacyanin metabolism in S. salsa calluses is regulated under white light conditions by growth regulators through the regulation of genes such as DODA that are involved in betacyanin synthesis.

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“…A regeneration system is a prerequisite for recovering transgenic plants. Many factors, including genotype (Choi et al, 2004;Obukosia et al, 2005;Xu et al, 2007), medium (Trigiano and Gray, 2000), explant type (Gambino et al, 2007), plant growth regulator (PGR) (Trigiano and Gray, 2000), and culture condition (Choi et al, 2001), can affect plant regeneration.…”

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confidence: 99%

Plant Regeneration of Periwinkle (Catharanthus roseus) via Organogenesis

Swanberg¹,

Dai²

2008

horts

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Two periwinkle cultivars, Pacific Coral (P1) and Sunstorm Rose (P2), were used for development of a plant regeneration system. Leaf and internodal explants collected from in vitro plants were plated onto woody plant medium (WPM) using a factorial arrangement of 6-benzyladeine (BA) and 1-naphthalene acetic acid (NAA). Shoots were successfully regenerated. Shoot production from leaf tissues was minimal for all cultivars, whereas internodal tissues showed variable rates of regeneration depending on the hormone combination. Cultivar P1 showed the maximum regeneration rate (73.3%) when internodal explants, 4 to 6 mm in length, were placed on WPM containing 5 μm BA and 5 μm NAA. Cultivar P2 showed a regeneration rate of 56.7% with a combination of 20 μm BA and 10 μm NAA. Shoot regeneration rate increased as the internodal explant size increased for P2; however, the regeneration rate decreased when the explant size was greater than 7 mm for P1. The shoot regeneration rate decreased as the period of the dark treatment of internodal explants increased in both P1 and P2. The antibiotics carbenicillin (Carb) and cefotaxime (Cef) had little effect on shoot regeneration. There was a slightly higher rate observed for P1 when Cef was added into the medium, whereas P2 showed a decrease with the addition of Cef. Carb showed no significant effect on shoot regeneration for both cultivars. Addition of both Carb and Cef to the medium slightly inhibited shoot regeneration.

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Effects of growth regulators and genotypes on pyrethrum In vitro

Cited by 12 publications

References 10 publications

Micropropagation of bromeliad Aechmea fasciata via floral organ segments and effects of acclimatization on plantlet growth

Micropropagation of bromeliad Aechmea fasciata via floral organ segments and effects of acclimatization on plantlet growth

Growth regulator-induced betacyanin accumulation and dopa-4,5-dioxygenase (DODA) gene expression in euhalophyte Suaeda salsa calli

Plant Regeneration of Periwinkle (Catharanthus roseus) via Organogenesis

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