Effects of heat treatment on hydrogen production potential and microbial community of thermophilic compost enrichment cultures

Silage as source of bacteria and electrons for dark fermentative hydrogen production

“…Duplicate samples were taken aseptically from the end points of hydrogen production experiments, samples were centrifuged (7500 g, 10 min) and the biomass was stored at À20 C. DNA was extracted with a PowerSoilä DNA isolation kit (MoBio Laboratories, Inc.). PCR-DGGE analysis, the reamplification of the bands as well as analysis of the sequence data were done as described by Nissilä et al [3].…”

“…methanogens. Methanogens can be inhibited, for example by heat-treatment [3] or chemicals [6] to increase hydrogen yields. Increasing substrate concentration has been reported to increase the hydrogen production rates [13,14] but to decrease hydrogen yields [15].…”

“…Hydrogen gas has many advantages; it has most energy per unit mass (122 MJ/kg), and it can be used for electricity production in fuel cells with water and heat as the only endproducts [1,2]. Dark fermentative hydrogen productions from both pure cellulosic materials, such as cellulose and xylan [3,4], and renewable cellulosic materials, such as food waste, sugarcane bagasse and paper mill waste [4e6], have been studied extensively. Grass silage is a potential cellulosic material for biological hydrogen production.…”

Dark fermentative hydrogen production from xylose by a hot spring enrichment culture

“…Partial bacterial 16S rRNA genes were amplified by using a primer pair GC-BacV3f and 907r as previously described by Koskinen et al [5] by using T3000 Thermocycler (Biometra). DGGE was performed with INGENY phorU2 x 2 esystem (Ingeny International BV, GP Goes, The Netherlands) as described by Nissilä et al [10]. The bands were re-amplified for sequencing as described by Koskinen et al [5] and sequence data was analyzed with Bioeditsoftware (version 7.0.5) and compared with sequences in GenBank (http://www.ncb.nlm.nih.gov/blast/).…”

Shifts in indigenous microbial communities during the anaerobic degradation of pentachlorophenol in upland and paddy soils from southern China

“…S6 in SI), but there was no direct evidence thus far about their involvement in PCP degradation. Coprococcus and Ruminiclostridium have always been reported to produce acetate via fermentative metabolisms (Tsai and Jones 1975;Patel et al 1981;Nissila et al 2011;Zhang et al 2014). The presence of this metabolic product might enhance the degradation rate of PCP (Chang et al 1995).…”