Effects of hMASP-2 on the formation of BCG infection-induced granuloma in the lungs of BALB/c mice

Xu, Xiaoying; Lü, Xiaoling; Dong, Xingfang; Luo, Yanping; Wang, Qian; Liu, Xun; Fu, Jie; Zhang, Yuan; Zhu, Bin; Ma, Xingming

doi:10.1038/s41598-017-02374-z

Cited by 9 publications

(8 citation statements)

References 42 publications

(32 reference statements)

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“…This result was also supported in two recent animal experiments. Two studies applied human-MASP-2 to mouse and rabbit models, respectively, and found protective effects of MASP-2 on TB infection 31 , 32 . Not only that, it has also been found in humans that mutations in certain sites of the MASP2 gene increase the susceptibility to TB.…”

Section: Discussionmentioning

confidence: 99%

Impact of MASP2 gene polymorphism and gene-tea drinking interaction on susceptibility to tuberculosis

Wang

Zhong

et al. 2021

Sci Rep

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Mannan-binding lectin-associated serine protease-2 (MASP-2) has been reported to play an important role as a key enzyme in the lectin pathway of the complement system. The objectives of our study were to determine whether the single-nucleotide polymorphism (SNPs) of MASP2 and the gene-tea drinking interaction were associated with the susceptibility to TB. In total, 503 patients and 494 healthy controls were contained. Three SNPs (rs12142107, rs12711521, and rs7548659) were genotyped. The association between the SNPs and susceptibility to TB were investigated by conducting multivariate unconditional logistic regression analysis. The gene-tea drinking interactions were analyzed by the additive model of marginal structural linear odds models. Both genotype AC + AA at rs12711521 of MASP2 genes and genotype GT + GG at rs7548659 of MASP2 genes were more prevalent in the TB patient group than the healthy control group (OR: 1.423 and 1.439, respectively, P < 0.05). In addition, The relative excess risk of interaction (RERI) between tea drinking and rs12142107, rs12711521, and rs7548659 of MASP2 genes was found to suggest negative interactions, which reached − 0.2311 (95% confidence interval (CI): − 0.4736, − 0.0113), − 0.7080 (95% CI − 1.3998, − 0.0163), and − 0.5140 (95% CI − 0.8988, − 0.1291), respectively (P < 0.05). Our finding indicated that the SNPs (rs12711521 and rs7548659) of MASP2 were associated with the susceptibility to TB. Furthermore, there were negative interactions between tea drinking and rs12142107, rs12711521, and rs75548659 of MASP2 gene, respectively. Our research provides a basis for studying the pathogenesis and prevention of tuberculosis.

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Section: Discussionmentioning

confidence: 99%

Impact of MASP2 gene polymorphism and gene-tea drinking interaction on susceptibility to tuberculosis

Wang

Zhong

et al. 2021

Sci Rep

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“…In the current study, fresh GC tumor tissues and adjacent normal gastric tissues were collected from patients who underwent surgical resection, blood samples were collected from GC patients diagnosed with primary GC without previously treatment, and paraffin-embedded GC tissues and adjacent noncancerous gastric tissues were obtained from patients who underwent resection between January 2014 and December 2015. Human peripheral blood mononuclear cells (PBMCs) were isolated from fresh blood using Ficoll-Hypaque density gradient centrifugation (Biochrom, Berlin, Germany) according to a previous study [ 16 ]. All of the included patients provided informed consent and signed an agreement.…”

Section: Methodsmentioning

confidence: 99%

microRNA-128-3p inhibits CD4+ regulatory T cells enrichment by targeting interleukin 16 in gastric cancer

et al. 2021

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Previous studies have confirmed that microRNA (miR)-128-3p is expressed at low levels in gastric cancer (GC), and low miR-128-3p expression promotes the growth of GC cells. However, whether the dysregulation of miR-128-3p expression affects tumor-infiltrating lymphocytes (TILs) and leads to immune escape remains unclear. In the present study, predictive bioinformatics approaches showed that miR-128-3p expression was inversely correlated with tumor-infiltrating lymphocyte enrichment. When CD4 + T cells and regulatory T cells (Tregs) were enriched, lower miR-128-3p expression was associated with worse overall survival. However, when numbers of CD8 + T cells were decreased, the upregulation of miR-128-3p expression had a favorable effect on GC prognosis. Dual-luciferase reporter assays and cell biology experiments revealed that interleukin 16 (IL16) was the target of miR-128-3p and was negatively regulated by miR-128-3p. In addition, GC cells were cocultured with T lymphocytes, and the subsequent flow cytometric analysis showed that overexpression of miR-128-3p in tumor cells decreased the percentages of CD4+ CD25+ Foxp3+ Tregs by downregulating IL16 expression in GC, whereas miR-128-3p inhibition had the opposite effect. Moreover, the recombinant IL16 reversed the effects of miR-128-3p overexpression, and a competitive antibody against the IL16 receptor CD4 also reversed the effects of miR-128-3p knockdown. These studies identified the mechanism by which the miR-128-3p/IL16 axis promotes the infiltration of CD4+ Tregs in GC, and this mechanism will be a promising therapeutic target in GC immunotherapy.

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“…A brown positive signal was quantitatively expressed as the integrated optical density (IOD), and the average IOD per field for individual lungs was calculated using Image-Pro Plus 6.0 (IPP6) software (Media Cybernetics, Inc., Bethesda, MD, USA). A comparison of the total IODs between each of the two groups was performed [20].…”

Section: Histopathological and Immunohistochemical Examinationmentioning

confidence: 99%

Mycobacterium tuberculosis YrbE3A Promotes Host Innate Immune Response by Targeting NF-κB/JNK Signaling

et al. 2020

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Mycobacterium tuberculosis is considered a successful pathogen with multiple strategies to undermine host immunity. The YrbE3A is encoded by Rv1964 within the RD15 region present in the genome of Mtb, but missing in M. bovis, M. bovis BCG (Pasteur) strain, and M. smegmatis (Ms). However, little is known about its function. In this study, the YrbE3A gene was cloned into pMV261 and expressed in Ms and BCG, while the strains with the vector served as the controls. The YrbE3A was expressed on the mycobacterial membrane, and the purified protein could stimulate RAW264.7 cells to produce IL-6. Furthermore, the effect of the recombinant strains on cytokine secretion by RAW264.7 was confirmed, which varied with the host strains. Ms_YrbE3A increased significantly higher levels of TNF-α and IL-6 than did Ms_vec, while BCG_YrbE3A enhanced higher TNF-α than BCG_vec. The pathways associated with NF-κB p65 and MAPK p38/JNK, other than Erk1/2, regulated this process. In addition, mice were infected with Ms_YrbE3A and Ms-vec and were kinetically examined. Compared to Ms-vec, Ms_YrbE3A induced more serious inflammatory damage, higher levels of TNF-α and IL-6, higher numbers of lymphocytes, neutrophils, and monocytes in a time-dependent way, but lower lung bacterial load in lung. These findings may contribute to a better understanding of Mtb pathogenesis.

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Effects of hMASP-2 on the formation of BCG infection-induced granuloma in the lungs of BALB/c mice

Cited by 9 publications

References 42 publications

Impact of MASP2 gene polymorphism and gene-tea drinking interaction on susceptibility to tuberculosis

Impact of MASP2 gene polymorphism and gene-tea drinking interaction on susceptibility to tuberculosis

microRNA-128-3p inhibits CD4+ regulatory T cells enrichment by targeting interleukin 16 in gastric cancer

Mycobacterium tuberculosis YrbE3A Promotes Host Innate Immune Response by Targeting NF-κB/JNK Signaling

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