Effects of Host Plant Factors on the Bacterial Communities Associated with Two Whitefly Sibling Species

Abstract

BackgroundAlthough discrepancy in the specific traits and ecological characteristics of Bemisia tabaci between species are partially attributed to the B. tabaci-associated bacteria, the factors that affect the diversity of B. tabaci-associated bacteria are not well-understood. We used the metagenomic approach to characterize the B. tabaci-associated bacterial community because the approach is an effective tool to identify the bacteria.Methodology and ResultsTo investigate the effects of the host plant and a virus, tomato yellow leaf curl virus (TYLCV), on the bacterial communities of B. tabaci sibling species B and Q, we analyzed the bacterial communities associated with whitefly B and Q collected from healthy cotton, healthy tomato, and TYLCV-infected tomato. The analysis used miseq-based sequencing of a variable region of the bacterial 16S rDNA gene. For the bacteria associated with B. tabaci, we found that the influence of the host plant species was greater than that of the whitefly cryptic species. With further analysis of host plants infected with the TYLCV, the virus had no significant effects on the B. tabaci-associated bacterial community.ConclusionsThe effects of different plant hosts and TYLCV-infection on the diversity of B. tabaci-associated bacterial communities were successfully analyzed in this study. To explain why B. tabaci sibling species with different host ranges differ in performance, the analysis of the bacterial community may be essential to the explanation.

Leukocyte-mediated Delivery of Nanotherapeutics in Inflammatory and Tumor Sites

“…In inflammation, neutrophils are the first arriving to inflammatory site and activate the host response. Neutrophils migrate from circulation to infected sites by crossing an endothelial layer, called neutrophil transmigration 36 and eliminate pathogens and protect the immunity 37 . The neutrophil recruitment is regulated by several adhesion molecules expressed on both neutrophils and endothelium 38 .…”

A change in the bacterial community of spider mites decreases fecundity on multiple host plants

“…In this study, the host plants tested strongly altered the composition of the microbial community (diversity and abundance) in spider mites. Similarly, research on Colorado potato beetles (Chung et al, 2017), whiteflies (Su et al, 2016), aphids (Guidolin & Cônsoli, 2016), and pine processionary moths (Strano et al, 2017) have shown that populations that fed on different plant species had differing microbial communities. Here, we found that spider mites that were switched from bean to other host plants experienced changes in bacterial community diversity and species richness, especially for the main bacterial species.…”

Development of Bemisia tabaci MEAM1 and MED on Tomato (Solanum lycopersicum) Alone and in a Mixed Population

“…We hypothesized that our test population of Eastern MED (Q2), collected from a residence in Palm Beach County, would demonstrate a competitive disadvantage on tomato in relation to MEAM1 as MED had in China. However, given that the pest status of MED populations can be influenced by endosymbiont fauna, host plant, and history of exposure to insecticides, it is difficult to predict how MED populations in Florida will behave based on information derived from countries where growing conditions are in many ways distinct from Florida (Fang et al 2014;Mouton et al 2014;Fujiwara et al 2015;Su et al 2016). In addition, we present the results of probit analysis for imidacloprid and thiamethoxam tolerance from 2 MED populations collected from hibiscus (Hibiscus rosa-sinensis L.; Malvaceae) in Palm Beach County, and 13 MEAM1 populations collected from commercial vegetable fields in south Florida between late 2016 and mid-2017.…”