Effects of hydraulic pressure on cardiomyoblasts in a microfluidic device

Hsiao, Yu-Fang; Pan, Huei-Jyuan; Tung, Yi-Chung; Chen, Chien‐Chang; Lee, Chau‐Hwang

doi:10.1063/1.4917080

Cited by 4 publications

(1 citation statement)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The same cell line was also used by Hsiao et al to investigate hydraulic pressure application, which resulted in bigger cell size and higher levels of natriuretic peptide. This effect could be reversed by a focal adhesion kinase blocker (Hsiao et al 2015). A microfluidic high-throughput platform incorporating microtissues from small cell numbers (~5000 cells per tissue) and pneumatic mechanical stimulation was developed as a model for cardiac hypertrophy with volume overload and recapitulated the upregulation of the foetal gene programme, associated with this pathology (Parsa et al 2017).…”

Section: Micro To Macromentioning

confidence: 99%

Tools for studying and modulating (cardiac muscle) cell mechanics and mechanosensing across the scales

Swiatlowska

Iskratsch

2021

Biophys Rev

View full text Add to dashboard Cite

Cardiomyocytes generate force for the contraction of the heart to pump blood into the lungs and body. At the same time, they are exquisitely tuned to the mechanical environment and react to e.g. changes in cell and extracellular matrix stiffness or altered stretching due to reduced ejection fraction in heart disease, by adapting their cytoskeleton, force generation and cell mechanics. Both mechanical sensing and cell mechanical adaptations are multiscale processes. Receptor interactions with the extracellular matrix at the nanoscale will lead to clustering of receptors and modification of the cytoskeleton. This in turn alters mechanosensing, force generation, cell and nuclear stiffness and viscoelasticity at the microscale. Further, this affects cell shape, orientation, maturation and tissue integration at the microscale to macroscale. A variety of tools have been developed and adapted to measure cardiomyocyte receptor-ligand interactions and forces or mechanics at the different ranges, resulting in a wealth of new information about cardiomyocyte mechanobiology. Here, we take stock at the different tools for exploring cardiomyocyte mechanosensing and cell mechanics at the different scales from the nanoscale to microscale and macroscale.

show abstract

Section: Micro To Macromentioning

confidence: 99%