Effects of hyperbaric oxygen therapy on the rat intestinal mucosa apoptosis caused by ischemia‐reperfusion injury

Abstract

To examine the apoptosis expression in the intestinal mucosa in accordance of different periods of hyperbaric oxygen (HBO) treatment, rats were submitted to 60 min of mesenteric artery and vein ischemia and 60 min of reperfusion occlusion. A group (G-IR) was the control and HBO was applied in the ischemia (GHBO-I), reperfusion (GHBO-R), and ischemia and reperfusion time (GHBO-IR). After 60 min of reperfusion, samples of small bowel were prepared for immunohistochemical expression of caspase-3. The expression of caspase-3 was significantly inferior when HBO was administered in the ischemia (0.16 +/- 0.01) in comparison with the control (0.70 +/- 0.08), but HBO in the further reperfusion (0.84 +/- 0.03) or both ischemia and reperfusion time (0.42 +/- 0.05) was significantly worse. There was a connection between HBO, small bowel I/R injury, and mucosa apoptosis. The favorable effect was obtained when HBO was administered early in the ischemia time.

Neuroprotective Effect of Hyperbaric Oxygen Therapy on Anterior Ischemic Optic Neuropathy

“…Others noted that the neuroprotective effect of HBO was dose-dependent and that HBO yielded better results than normobaric oxygen in terms of both clinical outcome and infarct volume reduction (Eschenfelder et al, 2008). Similar neuroprotective effects of HBO in ischemia-reperfusion injury were reported in skeletal muscle (Vidigal et al, 2007) and intestine (Bertoletto et al, 2007). In the ischemic reperfusion model of skeletal muscle, HBO treatment, applied during different periods, prevented interstitial hemorrhage, development of a neutrophil infiltrate, and cellular necrosis (Vidigal et al, 2007).…”

Effect of hyperbaric oxygen therapy on the intestinal ischemia reperfusion injury

“…HBOT has a beneficial effect on ischemia 23 . These parameters may also be associated with increased VEGF expression.…”

Morphological study of rat skin flaps treated with subcutaneous dimethyl sulfoxide combined with hyperbaric oxygen therapy

“…Previous studies confirmed our results suggesting that oxygen acts as a flag for a series of events that promote healing. HBO-treated tissues show a reduction in hypoxia-inducible factor 1-alpha (HIF-1a), which is an important gene expression regulator involved in the regulation of tissue oxygenation and which increases in the presence of hypoxia (Bertoletto et al, 2007). The protein levels of BNip3 (a pro-apoptotic protein located in the mitochondria) was markedly elevated from day 10 in ischemic tissues.…”