Effects of hyperleptinemia in rat saliva composition, histology and ultrastructure of the major salivary glands

Lamy, Elsa; Neves, Sandra Mara Alves da Silva; Ferreira, Juliana; Rodrigues, L.; Costa, Gustavo; Cordeiro, Carlos; Fialho, Luís; Lima, M.; Costa, Ana Rodrigues; Antunes, Célia M.; Lopes, Orlando; Amado, Francisco; Silva, Fernando Capela e

doi:10.1016/j.archoralbio.2018.08.005

Cited by 6 publications

(3 citation statements)

References 29 publications

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“…Small ruminants, including sheep, are categorized as an intermediate nutrient animal in which their grazing and browsing behaviour rely on food quality, fineness and availability (Hofmann, 1989). Diet selection includes the picking of some plants or their parts with the rejection of other undesirable plants (Lamy et al, 2012). The formerly published data have illustrated the presence of variability in the morphological characterizations of the tongue of the different ruminant species that is correlated to the nature of the available feeding material, ecological conditions and the feeding mechanism (Mahdy et al, 2021) with a neglected study of the tongue of the sheep.…”

Section: Discussionmentioning

confidence: 99%

Morphological features of the Egyptian Ossimi sheep tongue: New scanning electron microscopic insights into its papillary system adaptations to Egyptian ecological conditions

Abumandour

Morsy

Elghoul

2022

Anat Histol Embryol

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The current study aims to illustrate the gross and scanning electron microscopic characterizations and ultrastructural adaptation of the lingual papillary system of Egyptian Ossimi sheep to Egyptian ecological conditions. The tongue had three regions: the apex (with a slightly bifurcated tip), the body (subdivided into rostral and caudal parts) and the root (subdivided into rostral papillary and caudal non‐papillary parts). Torus linguae had two parts: the triangular rostral part (the caudal part of the body) and the quadrilateral wide part (the rostral part of the root). The lingual papillary system had mechanical (filiform, conical and lentiform) and gustatory (fungiform and circumvallate) types. Filiform papillae were heavily scattered on the dorsal surface of the apex, the rostral part of the body and the ventral surface of the papillary region of the tip. Filiform papillae had five subtypes (ventral and dorsal processed, triangular, leaf‐like and triangular‐processed papillae), while the conical papillae had three subtypes: two lingual (small, large) and one paralingual (elongated pointed), and the fungiform had two subtypes: the high‐density ovoid (on the ventral surface of the tip) and round papillae (on the dorsal surface) that possessed a high number of taste pores, not previously described. They ranged from 5 to 10 for ovoid and 25 to 25 for round papillae. Each circumvallate papilla had an ovoid bulb (with 2–5 taste pores) encircled by an annular groove and two pads (i.e. not described previously). The papillary system's regional divergence was specialized for their harsh and semi‐harsh diet.

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Section: Discussionmentioning

confidence: 99%

Morphological features of the Egyptian Ossimi sheep tongue: New scanning electron microscopic insights into its papillary system adaptations to Egyptian ecological conditions

Abumandour

Morsy

Elghoul

2022

Anat Histol Embryol

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“…Recombinant rat leptin (endotoxin level <0.10 EU per 1 μg of protein by the Limulus amoebocyte lysate method) (R&D Systems, Minneapolis, MN, USA) was reconstituted at 1 mg/mL in a sterile vehicle: 20 mM Tris-HCI, pH 8.0. Chronic leptin treatment was delivered by an osmotic mini-pump (model 2ML1; ALZET, Cupertino, CA, USA) with an initial leptin concentration of 0.33 μg/μl and an infusion rate of 10 μl/h for 8 days, according to a previously described protocol [ 22 ].…”

Section: Methodsmentioning

confidence: 99%

Hyperleptinemia results in systemic inflammation and the exacerbation of ischemia-reperfusion myocardial injury

Polyakova

Mikhaylov

Галагудза

et al. 2021

Heliyon

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Aim Hyperleptinemia potentiates the effects of many atherogenic factors, such as inflammation, platelet aggregation, migration, hypertrophy, proliferation of vascular smooth muscle cells, and endothelial cell dysfunction. The present study analysed the effects of long-term hyperleptinemia in an in vivo myocardial ischemia-reperfusion model to demonstrate whether the in vivo deleterious effect also affects cardiac structure and function. Main methods Rats were subcutaneously administered leptin for 8 days to estimate the involvement of the JAK/STAT pathway. Data from 58 male Wistar rats were included in the final analysis. Myocardial infarction (MI) was modelled by the 30-minute ligation of the main left coronary artery followed by 120-minute reperfusion. Hemodynamic measurements, electrocardiography monitoring, echocardiography, myocardial infarct size and area at risk, blood biochemical parameters, leptin, IL-6, TNF-alpha, FGF-21, and cardiomyocyte morphology were measured. The expression of JAK2, p-JAK2, STAT3, p-STAT3 was assessed by Western Blot analysis. Statistical analyses were performed using IBM SPSS Statistics v.26. Key findings Eight-day hyperleptinemia in rats leads to an increase in blood pressure and heart rate, myocardial hypertrophy, impaired LV function, the frequency of ischemic arrhythmias, dyslipidemia, systemic inflammation, and the size of induced myocardial infarction. Significance: The blockade of the JAK/STAT signalling pathway effectively reverses the negative effects of leptin, including increased blood pressure and total cholesterol.

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“…Recombinant rat leptin (endotoxin level <0.10 EU per 1 μg of protein by the limulus amoebocyte lysate method) (R&D Systems, Minneapolis, MN, USA) was reconstituted at 1 mg/mL in a sterile vehicle: 20 mM Tris-HCI, pH 8.0. Chronic leptin treatment was delivered by an osmotic mini-pump (model 2ML1; ALZET, Cupertino, CA, USA) with an initial leptin concentration of 0.33 μg/μl and an infusion rate of 10 μl/hour for 8 days, according to a previously described protocol [22]. JSI-124 (cucurbitacin I), a specific inhibitor of JAK/STAT, was purchased from Sigma-Aldrich (St. Louis, MO, USA).…”

Section: Drugsmentioning

confidence: 99%

Hyperleptinemia Results in Systemic Inflammation and the Exacerbation of Ischemia-Reperfusion Myocardial Injury: The Involvement of the JAK/STAT Pathway

Polyakova

Mikhaylov

Галагудза

et al. 2021

Preprint

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Hyperleptinemia potentiates the effects of many atherogenic factors, such as inflammation, platelet aggregation, migration, hypertrophy, proliferation of vascular smooth muscle cells, and endothelial cell dysfunction. The present study analysed the eﬀects of long-term hyperleptinemia in an in vivo myocardial ischemia-reperfusion model to demonstrate whether the in vivo deleterious effect also affects cardiac structure and function. Rats by were subcutaneously administered leptin for 8 days to estimate the involvement of the JAK/STAT pathway. Data from 58 male Wistar rats were included in the final analysis. Myocardial infarction (MI) was modelled by the 30-minute ligation of the main left coronary artery followed by 120-minute reperfusion. Hemodynamic measurements, electrocardiography monitoring, echocardiography, myocardial infarct size and area at risk, blood biochemical parameters, leptin, IL-6, TNF-alpha, FGF-21, and cardiomyocyte morphology were measured. Statistical analyses were performed using IBM SPSS Statistics v.26. Seven-day hyperleptinemia in rats led to increased an blood pressure and heart rate, myocardial hypertrophy, impaired LV function, an increased frequency of ischemic arrhythmias, dyslipidaemia, systemic inflammation, and an increased size of induced myocardial infarction. The blockade of the JAK/STAT signalling pathway effectively reversed the negative effects of leptin, including increased blood pressure and total cholesterol.

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Effects of hyperleptinemia in rat saliva composition, histology and ultrastructure of the major salivary glands

Cited by 6 publications

References 29 publications

Morphological features of the Egyptian Ossimi sheep tongue: New scanning electron microscopic insights into its papillary system adaptations to Egyptian ecological conditions

Morphological features of the Egyptian Ossimi sheep tongue: New scanning electron microscopic insights into its papillary system adaptations to Egyptian ecological conditions

Hyperleptinemia results in systemic inflammation and the exacerbation of ischemia-reperfusion myocardial injury

Hyperleptinemia Results in Systemic Inflammation and the Exacerbation of Ischemia-Reperfusion Myocardial Injury: The Involvement of the JAK/STAT Pathway

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