Effects of Hyriopsis cumingii polysaccharides on angiogenesis, macrophage chemotaxis, proliferation and phagocytosis

Abstract

Anti-angiogenic activities of crude Hyriopsis cumingii polysaccharides (HCPS) and its purified fractions (HCPS-1, HCPS-2 and HCPS-3) were evaluated in vivo using the chicken embryo chorioallantoic membrane (CAM) assay. The promoting effects of crude HCPS and its purified fractions on the chemotaxis, proliferation and phagocytosis of peritoneal macrophage were tested by cell model in vitro and cyclophosphamide-induced immuno-suppression animal model in vivo. The results showed that HCPS could significantly suppress the neovascularization of chicken embryo CAM and promote peritoneal macrophage migrating to monocyte chemotactic protein-1 (MCP-1), propagating and devouring sheep red blood cell (SRBC) in a dose-dependent manner. In addition, HCPS-3 showed stronger immunostimulatory activities in vitro than crude HCPS, HCPS-1 and HCPS-2. The beneficial effects of HCPS on the immune system might be, at least in part, attributed to the improvement of chemotaxis, proliferation and phagocytosis of peritoneal macrophage. All these results suggest that HCPS is a potential immunoenhancing and anti-tumor agent.

Structural Characterization and Immunostimulatory Activity of a Homogeneous Polysaccharide from Sinonovacula constricta

“…Polysaccharides and oligosaccharides have intrigued biologists for decades as therapeutic agents and mediators of complex cellular events. , There is increasing evidence that a great number of polysaccharides isolated from plants, fungi, and bacteria are associated with immunostimulatory effects and do not cause significant side effects . For example, glucans from edible mushrooms have been used as a nonspecific immune modulatory supplement, − and the polysaccharides from animals, mainly common bivalve mollusk, also possess immune activity. − It is well-known that the structure features of polysaccharides including their molecular weights, monosaccharide compositions, glycosidic bonds, branched degrees, and conformation are related to their various biological functions. ,, Moreover, the exact structure of a polysaccharide is a prerequisite to probe its potential mechanisms and structure–activity relationship. However, information about the purification, structural feature and immunostimulatory activity of SCP is not available.…”