Effects of <i>Phaseolus vulgaris</i> isolectins on the structure and function of the small intestinal mucosa in the self‐emptying blind‐pouch model in piglets

Kik, M. J. L.; Muysenberg, A. Van Den; Kleef, D.J. van

doi:10.1111/j.1439-0396.1995.tb00414.x

Cited by 4 publications

(3 citation statements)

References 25 publications

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“…Clearly, the systemic effects of a diet supplemented with food additives can only be studied in humans or animals (Kik et al 1990, Wachtershauser and Stein 2000 or in animals with a self-emptying jejunal loop or self-emptying blind pouch (Kik et al 1995). Organ culture of small intestinal tissue has also been used as an in vitro model to study the interaction of food additives with tissues (Trier 1980, Kik et al 1991), but this model has a serious drawback.…”

Section: Introductionmentioning

confidence: 99%

Differential modulation of enterocyte-like Caco-2 cells after exposure to short-chain fatty acids

Malago

Koninkx

Douma

et al. 2003

Food Additives and Contaminants

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The response of intestinal epithelial cells to short-chain fatty acids, which are increasingly used as food additives, was investigated. Human small intestinal epithelial cell model Caco-2 cells were exposed to formate, propionate and butyrate to assess their effect on cellular growth, metabolism, differentiation and protection against bacteria. The Caco-2 cells were entirely grown in the different short-chain fatty acids and respective growth patterns were determined. Differentiated cells were exposed to 0-20 mM short-chain fatty acids for 48 h and changes in DNA, RNA, (glyco)protein syntheses, sucrase isomaltase activity, transepithelial electrical resistance and protection against Salmonella enteritidis were measured. The short-chain fatty acids, altered linearly and differentially the growth pattern ranging from stimulation by formate to inhibition by butyrate. Formate inhibited cellular metabolism. Low concentrations of up to 5 mM propionate and 2 mM butyrate stimulated metabolism, while higher doses were inhibitory. Formate had no effect on sucrase isomaltase enzyme activity and transepithelial electrical resistance, whereas propionate and butyrate increased these markers of differentiation. Infection with S. enteritidis did not benefit from the short-chain fatty acid-induced transepithelial electrical resistance. It is concluded that formate, propionate and butyrate selectively and differentially modulate growth characteristics, cellular metabolism, sucrase isomaltase activity and transepithelial electrical resistance in a concentration- and carbon atom-related fashion. The short-chain fatty acid-induced transepithelial electrical resistance does not confer protection against S. enteritidis.

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Section: Introductionmentioning

confidence: 99%

Differential modulation of enterocyte-like Caco-2 cells after exposure to short-chain fatty acids

Malago

Koninkx

Douma

et al. 2003

Food Additives and Contaminants

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“…The villus height was taken as the distance from the crypt opening to the tip of the villus while the crypt depth was measured from the base of the crypt to the level of the crypt opening [ 16 ]. Five villi were selected from segment for each rat in the three groups for microscopic analysis, using a 10 × 4 magnifying lens.…”

Section: Methodsmentioning

confidence: 99%

Intestinal Fluid and Glucose Transport in Wistar Rats following Chronic Consumption of Fresh or Oxidised Palm Oil Diet

Obembe

Owu

Okwari

et al. 2011

ISRN Gastroenterology

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Chronic ingestion of thermoxidized palm oil causes functional derangement of various tissues. This study was therefore carried out to determine the effect of chronic ingestion of thermoxidized and fresh palm oil diets on intestinal fluid and glucose absorption in rats using the everted sac technique. Thirty Wistar rats were divided into three groups of 10 rats per group. The first group was the control and was fed on normal rat chow while the second (FPO) and third groups (TPO) were fed diet containing either fresh or thermoxidized palm oil (15% wt/wt) for 14 weeks. Villus height and crypt depth were measured. The gut fluid uptake and gut glucose uptake were significantly (P < .001) lower in the TPO group than those in the FPO and control groups, respectively. The villus height in the TPO was significantly (P < .01) lower than that in FPO and control. The villus depth in TPO was significantly (P < .05) higher than that in FPO and control groups, respectively. These results suggest that ingestion of thermoxidized palm oil and not fresh palm oil may lead to distortion in villus morphology with a concomitant malabsorption of fluid and glucose in rats due to its harmful free radicals.

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“…In addition to the binding of lectins to the epithelial cells, extensive endocytosis of soybean agglutinin and systemic absorption of PHA have been observed (Pusztai 1991). PHA can induce a variety of effects such as: 1) cell division in the lymphocytes of ewes (Banks et al 1978;Skoptes and Hansen 1993), chicken thymic and splenic T cells (Kline and Sander 1993), and human T cells (Varaldo et al 1989), 2) an increase in the population of microbial flora within the mucous layer of rat small intestine Chrispeels and Raikhel 1991), 3) atrophy and blunting of villi and irregular positioning of the microvilli (Kik et al 1995), 4) increases in the intracellular Ca 2+ of gut cells (Grissmer et al 1992), 5) inhibition of the absorption and transport of glucose (Chrispeels and Raikhel 1991;Santiago et al 1993) 6) uptake of polyamine in the small intestine of rat (Pusztai et al 1989; Bardocz et al 1990), and 7) an increase in the activities of membrane enzymes (Erikson et al 1985;Embaye et al 1995).…”

Section: Introductionmentioning

confidence: 96%

Subcellular effects and localization of binding sites of phytohemagglutinin in the potato leafhopper, Empoasca fabae (Insecta: Homoptera: Cicadellidae)

Habibi

Backus

Czapla

1998

Cell and Tissue Research

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To identify the means by which phytohemagglutinin (PHA) exerts its toxicity on the potato leafhopper, four different methods (thick and semi-thin sectioning combined with immunofluorescent staining, in vitro receptor autoradiography, and immunoelectron microscopy) were used to elucidate the PHA target tissue, binding site, and its effects on this tissue. Sixteen 1- or 2-day-old female potato leafhoppers were fed for 36 h on each of three treatments: a control, diet or a diet containing either the PHA-E subunit or the PHA-L subunit. The PHA-E subunit, but not PHA-L, had previously been shown to be lethal. The insects were then prepared for both light and confocal microscopy. Analysis of images showed that PHA bound only to the surface of midgut epithelial cells of the potato leafhopper. PHA-E caused severe disruption, disorganization, and elongation of the brush border microvilli, and swelling of the epithelial cells into the lumen of the gut, leading to complete closure of the lumen. Furthermore, PHA-E stimulated the division of midgut epithelial cell nuclei, leading to two nuclei in each cell. Nuclei later elongated and degraded. In contrast, PHA-L had little effect on the epithelial cells of the midgut. It did not strongly bind to the surface of epithelial cells and caused much less disruption of brush-border microvilli, less disorganization of the cells and less elongation of nuclei. Strong binding of PHA occurred solely on the cell membrane of the brush border microvilli of epithelial cells. In contrast, the controls (i.e., midgut tissue, blocking agent, PHA, and antibodies) showed that midgut tissue was not autofluorescent and showed no fluorescent binding signal. Analysis of both bright- and dark-field images obtained by autoradiography and immunoelectron microscopy confirmed these findings.

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Effects of Phaseolus vulgaris isolectins on the structure and function of the small intestinal mucosa in the self‐emptying blind‐pouch model in piglets

Cited by 4 publications

References 25 publications

Differential modulation of enterocyte-like Caco-2 cells after exposure to short-chain fatty acids

Differential modulation of enterocyte-like Caco-2 cells after exposure to short-chain fatty acids

Intestinal Fluid and Glucose Transport in Wistar Rats following Chronic Consumption of Fresh or Oxidised Palm Oil Diet

Subcellular effects and localization of binding sites of phytohemagglutinin in the potato leafhopper, Empoasca fabae (Insecta: Homoptera: Cicadellidae)

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