Effects of implant Suprelorin on cytological changes of vaginal epithelial cells in female dogs (4.7 mg deslorelin)

Abstract

Diagnosis of the estrous cycle in female dogs is important in veterinary medicine. The estrous cycle is divided into the following stages: proestrus, estrus, diestrus, metestrus, and anestrus, which due to the action of hormones, replace one after another. This feature of the estrous cycle leads to some difficulties with reproduction. It is hard to obtain newborns because of prolonged anestrus. Vaginal cytology is a simple and available diagnostic method of the estrous cycle. It is based on the analysis of vaginal mucosa epithelial cells changes. This method is used to determine the time of mating and diagnose pathological conditions in the reproductive system of female dogs. There are various drugs in veterinary medicine for the correction of the estrous cycle in female dogs. We used implant Suprelorin® to estrus induction. There were described methods of estrus stimulation using a subcutaneous implant Suprelorin (4.7 mg, deslorelin) and changes in vaginal epithelial cells using the vaginal cytology method. The study was performed on twelve (n = 12) adult female dogs in the anestrus stage. Implants were injected subcutaneously in the groin area and removed at the beginning of the estrus.

Cyto-morphological Changes in Exfoliated Vaginal Cells and Thermal Rhythms of Red Sokoto does during the Oestrous cycle

“…In the present study, vaginal smears at the proestrus stage, showed a rise in the proportion of anuclated superficial cells and nucleated superficial cells, with a reduction in intermediate and parabasal cells. This observation is in agreement with the reports (Reddy et al, 2011;Holumbiyovska et al, 2021) for the dog and Sitaresmi et al (2019) in does. Also, the few numbers of parabasal and intermediate cells that were observed, is consistent with the findings of Sire-gar et al (2016); but these observations were at variance with that of Kustritz (2012), who reported very high proportions of intermediate cells and few nucleated superficial cells at proestrus stage.…”

“…The estrous cycle represents a complex neurohumoral process characterized by morphological and physiological changes in some of the reproductive organs, such as the vagina. These cyto-morphological changes in exfoliated cells in the vaginal smears, fairly reflect the underlying endocrine events of the reproductive cycle, due to sensitivity of vaginal epithelium to stimulation by sex hormones (Kinoshita et al, 2012;Peña-Corona et al, 2019;Holumbiyovska et al, 2021) and are, thus, considered as a better predictor of the breeding time.…”

“…Research relating to cyclical changes in exfoliated vaginal cytology used to characterize the estrous cycle have been reported in women (Papanicolau, 1963;Berga et al, 2019), rats (Paccola et al, 2013;Cora et al, 2015) and also, in a variety of domestic animal species, including the bitch (Pérez et al, 2005;Maenhoudt et al, 2018), queen (Kustritz, 2012;Kanca et al, 2014), bovine (Siregar et al, 2016;Holumbiyovska et al, 2021), ewes (Solis et al, 2008;Zohara et al, 2014), does (Ola et al, 2006;Leigh et al, 2010;Ribeiro et al, 2019). The results of some of these studies were used clinically in the detection of estrus and early pregnancy (Reddy et al, 2011;Sharma and Sharma, 2016;Antonov, 2017), and as well as, in management of reproductive disorders and genital tract infections (Sharma and Sharma, 2016;Llazani et al, 2021).…”

Changes in serum progesteron and 17beta-estradiol concentration after application of Suprelorin (4,7 deslorelin acetate) implant for estrus induction in bitches

“…Результати цитологічного дослідження епітелію піхви наведені у нашій попередній публікації (Holumbiyovska et al, 2021).…”

“…Також варто зазначити, що у окремих випадках цитологічна картина епітелію піхви може не вказувати на ознаки тічки. Цитологічно у дослідній групі фіксували прояви проеструсу починаючи з третьої доби після імплантації (Holumbiyovska et al, 2021), що не завжди було помітно у сук з контрольної групи. Детальні результати цитологічних змін епітелію піхви після застосування імпланту Супрелорін наведені в нашій попередній статті (Holumbiyovska et al, 2021).…”