Effects of Jamaican cherry (Muntingia calabura L.) Fruits Extract on Immunoglobulin G Levels and Hematological Profiles in Mice

Abstract

Background: Jamaican cherry (Muntingia calabura L.) fruits contain potential immunomodulatory agents such as phenolic and flavonoid compounds. However, previous research was limited only to the effect of Jamaican cherry fruits on non-specific immune response namely the phagocytic index. Objective: This study aims to determine the immunomodulatory activity of Jamaican cherry fruits against specific immune response by measuring the antibody formation named immunoglobulin G (IgG). In addition, the hematological profile of the animals induced by hepatitis B vaccine was also evaluated. Materials and methods: forty male mice were grouped into 8 groups consist of control, treatment, and standard groups. Control group was given CMC-Na 0.5%, six treatment groups were each given either the methanol extract of Jamaican cherry fruits (MEJF), or ethyl acetate fraction of Jamaican cherry fruits (EAJFJ) with the doses of 50, 100, and 200 mg/kg BW (each dose for one group), and levamisole was given to the standard group. Levamisole and extract of Jamaican cherry fruits were prepared as a suspension in CMC-Na 0.5% and administered orally for 15 days. Mice were induced with hepatitis B vaccine intraperitoneally twice. The levels of IgG, hematological profiles, and organ index were measured on the 16 th day. Results: The administration of MEJF and EAFJF stimulated IgG production significantly compared to the control group (p<0.05). Meanwhile, the MEJF and EAFJF did not influence the hematological profile. An increase of the spleen index was found in the EAFJF group compared to the control. Both MEJF and EAFJF were shown to have flavonoids, phenolics, and triterpenoids contents. Conclusion: MEJF and EAFJF possessed immunostimulant properties by increasing the specific (humoral) immune response (IgG) after induction with the hepatitis B vaccine.

Traditional, Current and Prospective Therapeutic Uses of Muntingia calabura: A Comprehensive Literature Review

“…However, the given genus exhibits characteristics that would be atypical in either family, this is why this placement is not widely accepted by specialists. Similarly, a distinct family with no ties to other families 19,20 .…”

UJI EFEK IMUNOSTIMULATOR DAUN Muntingia calabura L TERHADAP SEL FAGOSITOSIS

“…Kebanyakan turunan fenolik pada tumbuhan mempengaruhi respon imun non spesifik terutama melalui peningkatan fagositosis dan limfosit serta proliferasi neutrofil (Grigore, 2017). Penelitian sebelumnya menunjukkan bahwa ekstrak metanol dan fraksi etil asetat memiliki efek imunomodulator terhadap respon imun non spesifik dengan meningkatkan indeks fagositik (Sujono et al, 2020).…”

Phytochemical Investigation and Antimicrobial Activity of Muntingia calabura L. Against Selected Pathogens

“…In addition, the leaves were also reported to have properties including anti-gout, antidiabetic, antihypertensive, antioxidant, and antiinflammatory (11,12). M. calabura fruits are well-known to possess certain pharmacological activities such as anti-inflammatory properties, as well as antirheumatic, antioxidants, antidiabetic, and immunomodulatory properties (13). In Central America, its flowers are traditionally used as antispasmodic and antiseptic agents, and some compounds isolated from its roots may be used to control malignant cell growth (14).…”