Effects of Jobelyn® on Isoniazid-Induced Seizures, Biomarkers of Oxidative Stress and Glutamate Decarboxylase Activity in Mice

Asehinde, Stephen; Ajayi, Abayomi Mayowa; Bakre, Adewale G.; Omorogbe, Osarume; Adebesin, Adaeze; Umukoro, Solomon

doi:10.32598/bcn.9.6.389

Cited by 6 publications

(7 citation statements)

References 25 publications

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“…Isoniazid at high doses inhibits GABA synthesis and depletes the GABA contents of the brain. Consequently, this drug can induce seizures by inhibiting GABA transmission ( Asehinde et al, 2018 ; Patil et al, 2011 ). However, we used low doses of isoniazid that unlike the high doses can increase the GABA levels in the brain ( Perry & Hansen, 1973 ).…”

Section: Discussionmentioning

confidence: 99%

“…One of the main mechanisms by which isoniazid influences the brain is the modulation of the GABAergic system. Research indicates that isoniazid in high doses decreases the GABA levels of the brain and induces convulsions ( Asehinde et al, 2018 ; Gupta, Dua, Kazmi, & Anwar, 2014 ; Patil, Patil, Patil, & Jadhav, 2011 ). However, some studies showed that low-dose isoniazid has GABA-elevating effects ( Perry & Hansen, 1973 ; Perry, Urquhart, Hansen, & Kennedy, 1974 ).…”

Section: Introductionmentioning

confidence: 99%

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Effects of Isoniazid on Tolerance and Sensitization to the Rewarding Properties of Morphine: A Conditioned Place Preference Procedure Investigation in Mice

Barzegari

Shahabi

2020

Basic Clin. Neurosci. J.

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Introduction: The GABAergic system of the brain plays a key role in morphine tolerance and sensitization. As isoniazid is a modulator of the GABAergic system, the present study aims to understand whether isoniazid can influence the induction of tolerance and sensitization to the rewarding effects of morphine. Methods: The rewarding effects of morphine and isoniazid were assessed using a Conditioned Place Preference (CPP) procedure in female mice. Tolerance to the rewarding effects of morphine was induced with high-dose morphine (25 mg/kg, SC), twice a day, for four days. Also, the sensitization was induced with an effective dose of morphine (5 mg/kg, SC), once a day, for three days. During the induction of tolerance or sensitization, the different groups of mice received saline or isoniazid (25, 50, and 75 mg/kg, IP) one hour before each morphine injection. Results: Morphine (0.5–10 mg/kg, SC) produced a significant CPP, but isoniazid (25, 50, and 75 mg/kg, IP) did not induce place preference or place aversion in mice. Although an effective dose of morphine (5 mg/kg, SC) did not induce CPP in morphine tolerated mice, an ineffective dose (0.5 mg/kg, SC) could produce a significant CPP in morphine-sensitized animals. The administration of isoniazid before morphine (on the days of tolerance or sensitization induction) inhibited the development of tolerance or sensitization to the rewarding effect of morphine in the CPP paradigm. Conclusion: Isoniazid can be a useful drug for the prevention of tolerance and sensitization to the rewarding effects of morphine.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effects of Isoniazid on Tolerance and Sensitization to the Rewarding Properties of Morphine: A Conditioned Place Preference Procedure Investigation in Mice

Barzegari

Shahabi

2020

Basic Clin. Neurosci. J.

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“…Isoniazid-Induced Seizure Model. The method was as previously described [19]. Animals were adapted to the chamber for 1 h before receiving treatment with either CPE (30-300 mg/kg, p.o.…”

Section: Plant Collection and Extractionmentioning

confidence: 99%

The Anticonvulsant Effect of Hydroethanolic Leaf Extract of Calotropis procera (Ait) R. Br. (Apocynaceae)

et al. 2021

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A number of currently used drugs have been obtained from medicinal plants which are a major source of drugs. These drugs are either used in their pure form or modified to a semisynthetic drug. Drug discovery through natural product research has been fruitful over the years. Traditionally, Calotropis procera is used extensively in the management of epilepsy. This study is conducted to explore the anticonvulsant effect of a hydroethanolic leaf extract of Calotropis procera (CPE) in murine models. This effect was evaluated using picrotoxin-induced convulsions, strychnine-induced convulsions, and isoniazid- and pilocarpine-induced status epilepticus in mice of both sexes. The results showed that CPE (100-300 mg/kg) exhibited an anticonvulsant effect against strychnine-induced clonic seizures by significantly reducing the duration ( p = 0.0068 ) and frequency ( p = 0.0016 ) of convulsions. The extract (100-300 mg/kg) caused a profound dose-dependent delay in the onset of clonic convulsions induced by picrotoxin ( p < 0.0001 ) and tonic convulsions ( p < 0.0001 ) in mice. The duration of convulsions was reduced significantly also for both clonic and tonic ( p < 0.0001 ) seizures as well. CPE (100-300 mg/kg), showed a profound anticonvulsant effect and reduced mortality in the pilocarpine-induced convulsions. ED50 (~0.1007) determined demonstrated that the extract was less potent than diazepam in reducing the duration and onset of convulsions but had comparable efficacies. Flumazenil—a GABAA receptor antagonist—did not reverse the onset or duration of convulsions produced by the extract in the picrotoxin-induced seizure model. In isoniazid-induced seizure, CPE (300 mg kg1, p.o.) significantly ( p < 0.001 ) delayed the onset of seizure in mice and prolonged latency to death in animals. Overall, the hydroethanolic leaf extract of Calotropis procera possesses anticonvulsant properties.

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“…It is necessary to create antiepileptic medicines (AEDs) that are more effective, safer, and have improved clinical characteristics. Status epilepticus (SE), also known as an isoniazidinduced seizure, is a serious disorder marked by frequent convulsive episodes that does not respond well to the currently available anticonvulsant drugs [5]. Status Epilepticus (SE) is one of the major side effects of isoniazid, a first line medication used for the treatment of tuberculosis.…”

Section: Introductionmentioning

confidence: 99%

Anticonvulsant Activity of Methanol Extract of Harungana madagascariensis Leaf on Mice Model of Isoniazid-induced Siezure

Celestine

Jude²,

Asogwa³

et al. 2022

JAMPS

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Background: Epilepsy is a neurological illness that affects people of all ages and is characterized by excessive electric activity in the brain. This causes social embarrassment coupled with side effect of orthodox medication; hence, the needs to search for plant with antiepileptic agent. Aims: This study aimed at investigating the anticonvulsive effects of Methanol extract of Harungana madagascriensis leaves on the isoniazid-induced (300 mg/kg, i.p) seizure in adult mice. Study Design: This is an original research carried out in the Department of Pharmacology, Faculty of pharmaceutical Sciences, Enugu State University of Science and Technology (ESUT), Agbani, Enugu State, Nigeria, between Jan and June, 2021. Methodology: The pulverized leaf of Harungana. madagascariensis was extracted using cold maceration and the phytochemical screening was carried out by the method of Treas and Evans The acute toxicity study was evaluated by the method of Lorke’s and Anticonvulsant study was carried by the method of Webster and Velluci. Data generated was statistically analyzed using one way ANOVA. Results: Preliminary phytochemical screening revealed the presence of flavonoids, alkaloids, phenols, glycosides, saponins, terpenoids and steroids. Flavonoids, Phenols, and terpenoids appeared in abundant concentration (4264.00 ±360.2,14065.00 ±538.4, 5484.00±30.4). Acute toxicity tests showed no toxicity and mortality at doses up to 5000 mgkg-1. Anticonvulsant study revealed that the extract significantly (p =.05) delayed the onset of clonic seizure in a dose dependent manner and abridge the duration of siezure on the group treated with 100, 500 and 1000 mg/kg b.w of extract compared with normal control when induced with isoniazid (300 mg/kg, i.p.). Conclusion: The results suggest that methanol extract of Harungana. madagascariensis leaves may have anticonvulsant activity, coupled with the presence of active secondary metabolites such as saponins, steriods and flavoniods which has a potentials for the management of epilepsy.

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Effects of Jobelyn® on Isoniazid-Induced Seizures, Biomarkers of Oxidative Stress and Glutamate Decarboxylase Activity in Mice

Cited by 6 publications

References 25 publications

Effects of Isoniazid on Tolerance and Sensitization to the Rewarding Properties of Morphine: A Conditioned Place Preference Procedure Investigation in Mice

Effects of Isoniazid on Tolerance and Sensitization to the Rewarding Properties of Morphine: A Conditioned Place Preference Procedure Investigation in Mice

The Anticonvulsant Effect of Hydroethanolic Leaf Extract of Calotropis procera (Ait) R. Br. (Apocynaceae)

Anticonvulsant Activity of Methanol Extract of Harungana madagascariensis Leaf on Mice Model of Isoniazid-induced Siezure

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