Effects of Kalsis, A Dietary Supplement, on Bone Metabolism in the Ovariectomized Rats

Abstract

We studied the ability of Kalsis, a food supplement that contains selenium, citric acid, and vitamin E, to prevent the effects of ovariectomy on bone loss. Six-month-old, Wistar female rats were studied. Groups (n = 12): SHAM: sham-operated rats; OVX: ovariectomized rats, treated with vehicle; OVX + Kalsis: ovariectomized rats treated with Kalsis (25 mg/kg/day) for 3 months. Bone mineral density (BMD) was determined by DXA in lumbar spine and femur. Computerized microtomography (μCT) in femur and serum osteocalcin (BGP), aminoterminal propeptide of procollagen I (PINP), β-isomer of carboxyterminal telopeptide of collagen I (CTX), and 5b isoenzyme of tartrate-resistant acid phosphatase (TRAP) were performed. Treatment with Kalsis prevented BMD loss in OVX group. μCT showed a decrease in BV/TV, and trabecular number, and an increase in trabecular separation in OVX rats. Kalsis administration attenuated partially bone loss observed by μCT due to ovariectomy. BGP, PINP, and the resorption index (CTX/TRAP) were increased in OVX group. Treatment with Kalsis maintained this increase. The mechanism of action of this supplement is not through a decrease in bone remodelling rate. The antioxidant action of this food supplement, due to the synergism of all its components, as a cause of its beneficial effect is suggested.

Strontium-loaded titania nanotube arrays repress osteoclast differentiation through multiple signalling pathways: In vitro and in vivo studies

“…In addition, the µCT evaluation revealed more trabecular bone with the anodised implants, particularly the NT-Sr3h sample, than with Ti. Consistent with a previous study 38 , ovariectomy produced a significant increase in bone remodelling, in terms of both resorption (CTX-I) and formation (PINP). The current study also demonstrates that NT-Sr decreases the CTX-I levels induced by OVX.…”

Alpha‐Lipoic Acid Promotes Osteoblastic Formation in H2O2‐Treated MC3T3‐E1 Cells and Prevents Bone Loss in Ovariectomized Rats

“…The number of slices was 20, and the total volume analyzed was 4.84 mm 3 . The procedures were performed following a method that was previously described (Montero et al, ).…”

An Open-label Randomized Clinical Study to Compare the Effects of a Nutritional Supplement versus Vitamin E on Fibroscan Score in Nonalcoholic Steatohepatitis (NASH) Patients

“…Figure 3C. Mean fibrosis scores at baseline and after 3 months of treatment in both the groups Viusid ® is a powerful antioxidant because of the Antioxidant activity of glycyrrhizic acid is over 100 times stronger than that of Vitamin E and the antioxidant capacity of Viusid ® is further increased by "Molecular Activation", which is about 800% more potent than its previous non-activated state [9,10,11,12]. Evidences showed that Viusid ® inhibits oxidative stress, lipid per-oxidation (MDA) and several pro-inflammatory cytokines (TNF-alpha, IL-1 &IL-6) involved in fibrogenesis process.…”

“…Antioxidant activity of glycyrrhizic acid, the most important active ingredient of the supplement, was found to be over 100 times stronger than that of Vitamin E [10]. Furthermore, the manufacturer used a patented bio-technology namely "Molecular Activation" to activate and increase the overall antioxidant activity of Viusid ® (before activation the antioxidant capacity was 1.1 points and after activation the antioxidant capacity significantly increased to 9.9 points) which is about 800% more potent than its previous non-activated state ( Figure 1) [9,11,12]. Evidences showed that Viusid ® inhibits oxidative stress, lipid per-oxidation (MDA) and several pro-inflammatory cytokines (TNF-alpha, IL-1 &IL-6) involved in fibrogenesis process.…”