Effects of L-glutamine on boar sperm quality during liquid storage at 17°C

Wang, Shunwei; Sun, Meng; Wang, Na; Yang, Kang; Guo, Hai-tao; Wang, Jingran; Zhang, Yuting; Shun-li, Yue; Zhou, Jiabo

doi:10.1016/j.anireprosci.2018.02.011

Cited by 20 publications

(15 citation statements)

References 26 publications

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“…Accumulation of ROS occurs during liquid storage of boar semen, which results in oxidative stress to sperm when it overwhelms the antioxidant defense [ 9 ]. Hence, the addition of extrinsic antioxidants may efficiently protect sperm from oxidative stress and extend their lifespan during storage.…”

Section: Discussionmentioning

confidence: 99%

“…However, the sperm fertility decreases with the extension of preservation [ 6 , 7 ]. One of the major issues is the accumulation of reactive oxygen species (ROS) [ 6 , 8 , 9 , 10 ]. The uncontrolled and excessive production of ROS that overwhelms the limited antioxidant defense system in sperm and seminal plasma leads to oxidative stress to sperm [ 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

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Proline Protects Boar Sperm against Oxidative Stress through Proline Dehydrogenase-Mediated Metabolism and the Amine Structure of Pyrrolidine

Feng

Zhu

Bai

et al. 2020

Animals

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Proline was reported to improve sperm quality in rams, stallions, cynomolgus monkeys, donkeys, and canines during cryopreservation. However, the underlying mechanism remains unclear. The aim of this study was to investigate the effect of proline on boar semen during liquid storage at 17 °C and explore the underlying mechanism. Freshly ejaculated boar semen was supplemented with different concentrations of proline (0, 25, 50, 75, 100, 125 mM) and stored at 17 °C for nine days. Sperm motility patterns, membrane integrity, ATP (adenosine triphosphate), reactive oxygen species (ROS), and GSH (glutathione) levels, and the activities of catalase (CAT) and superoxide dismutase (SOD) were evaluated after storage for up to five days. It was observed that boar sperm quality gradually decreased with the extension of storage time, while the ROS levels increased. Addition of 75 mM proline not only significantly improved sperm membrane integrity, motility, and ATP levels but also maintained the redox homeostasis via increasing the GSH levels and activities of CAT and SOD. When hydrogen peroxide (H2O2) was used to induce oxidative stress, addition of proline significantly improved sperm quality and reduced ROS levels. Moreover, addition of proline also improved sperm quality during the rapid cooling process. Notably, addition of DL-PCA (DL-pipecolinic acid) rescued the reduction of progressive motility and total motility caused by H2O2, and THFA (tetrahydro-2-furoic acid) failed to provide protection. Furthermore, addition of proline at 75 mM increased the activity of proline dehydrogenase (PRODH) and attenuated the H2O2-induced reduction in progressive motility. These data demonstrate that proline protects sperm against oxidative stress through the secondary amine structure and proline dehydrogenase-mediated metabolism.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Proline Protects Boar Sperm against Oxidative Stress through Proline Dehydrogenase-Mediated Metabolism and the Amine Structure of Pyrrolidine

Feng

Zhu

Bai

et al. 2020

Animals

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“…The semen was transported to the laboratory at 37–38°C within 30 min and filtered. Only semen samples with more than 70% sperm TMOT and <15% morphologically abnormal spermatozoa were used (Wang et al, ). Forty‐eight ejaculates were used for this experiment.…”

Section: Methodsmentioning

confidence: 99%

Protective influence of rosiglitazone against time‐dependent deterioration of boar spermatozoa preserved at 17°C

Wang

Yang

Guo

et al. 2019

Reprod Domestic Animals

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Spermatozoa are highly specialized cells, and energy metabolism plays an important role in modulating sperm viability and function. Rosiglitazone is an antidiabetic drug in the thiazolidinedione class that regulates metabolic flexibility and glucose uptake in various cell types, but its effects on boar sperm metabolism are unknown. In this study, we investigated the potential effect of rosiglitazone against time‐dependent deterioration of boar spermatozoa during liquid preservation at 17°C. Freshly ejaculated semen was diluted with Beltsville Thawing Solution (BTS) containing different concentrations of rosiglitazone, and the motility, membrane and acrosome integrity of sperm were detected. Besides, we measured glucose uptake capacity, l‐lactate production level, mitochondrial membrane potential, adenosine triphosphate (ATP) content and mitochondrial reactive oxygen species (mROS) production of sperm after boar semen had been incubated with or without rosiglitazone, iodoacetate (glycolysis inhibitor) and rotenone (electron transport chain inhibitor) for 5 days. The addition of rosiglitazone significantly enhanced sperm quality and had a strong protective effect on the sperm membrane and acrosome integrity during storage. BTS containing 50 μM rosiglitazone maintained the total motility of liquid‐preserved sperm above 60% for 7 days. Rosiglitazone improved sperm quality by regulating energy metabolism manner of preserved sperm, protected the sperm mitochondrial membrane potential, enhanced sperm ATP production and in the meanwhile reduced mROS through enhancing glycolysis but not oxidative phosphorylation. The data suggested the practical feasibility of using rosiglitazone for improving boar spermatozoa quality during semen preservation.

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“…The concentration of ROS in pig semen at room temperature will accumulate over time, which is a major factor threatening the quality of pig semen [14,26]. In our study, we found that the activity of pig sperm stored with hydroxytyrosol was better than control group from the second day of storage and the activity of sperm in the group of 120 µmol/L hydroxytyrosol was the best.…”

Section: Discussionmentioning

confidence: 52%

Hydroxytyrosol effectively improves the quality of pig sperm at room temperature

Zhang

Liu

Tian

et al. 2020

Preprint

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Background: Artificial insemination technology is the production technology used in more than 99% of the world's pig farms. Preservation of semen is mainly conducted at room temperature (17°C), during which the accumulation of reactive oxygen species (ROS). In our research, by adding different concentrations of hydroxytyrosol to the diluent during the storage of boar semen at 17°C, we compared them with the traditional diluent to study the cumulative amount of ROS, the extent of membrane damage, the sperm acrosome integrity, the activity of antioxidant enzymes, and other indicators to evaluate the effect of hydroxytyrosol on the changes of active oxygen and sperm quality during storage. After we determined the optimal amount of hydroxytyrosol in the diluent, Proteomics was used to monitor difference in sperm protein expression between the control group and the addition of 120 μmol/L hydroxytyrosol (optimum concentration) group after three days’ storage. Ultimately, we selected the control group and the addition of 120 μmol/L hydroxytyrosol group to test the effect of artificial insemination.Results: During storage of pig semen at room temperature, the sperm quality of the hydroxytyrosol-treated group was significantly improved (P < 0.05). Besides, its antioxidant capacity was also significantly improved (P < 0.05). Proteomics sequencing analysis proved that the addition of hydroxytyrosol treatment group has potential value in improving sperm cell quality. The significant increase in sow pregnancy rate and the increase in piglet birth weight also confirmed that sperm quality during storage can be of vital importance in actual production (P < 0.05).Conclusions: Based on our results, we demonstrated that the addition of hydroxytyrosol to the diluent can not only improve the quality of pig sperm, but also has an irreplaceable role in improving the efficiency of artificial insemination.

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Effects of L-glutamine on boar sperm quality during liquid storage at 17°C

Cited by 20 publications

References 26 publications

Proline Protects Boar Sperm against Oxidative Stress through Proline Dehydrogenase-Mediated Metabolism and the Amine Structure of Pyrrolidine

Proline Protects Boar Sperm against Oxidative Stress through Proline Dehydrogenase-Mediated Metabolism and the Amine Structure of Pyrrolidine

Protective influence of rosiglitazone against time‐dependent deterioration of boar spermatozoa preserved at 17°C

Hydroxytyrosol effectively improves the quality of pig sperm at room temperature

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