Effects of lead chloride on human erythrocyte membranes and on kinetic anion sulphate and glutathione concentrations

Gugliotta, T.; Luca, G. De; Romano, P.; Rigano, Caterina; Scuteri, A; Romano, L.

doi:10.2478/s11658-012-0027-2

Cited by 10 publications

(9 citation statements)

References 41 publications

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“…The increases in the levels of LPO in our Pd-exposed rats are thus directly indicating that Pd-poisoning induces a dropping down of the antioxidative defense and leads to hemolysis. Gugliotta et al (2012) [ 41 ] reported that the exposure of erythrocytes to Pb leads to a reduction in the average lifetime of the erythrocytes and the subsequent development of anemia. Though the mechanism(s) is not clearly known, one important effect of lead toxicity in erythrocytes consists of increasing intracellular calcium [Ca 2+ ](i), which in turn causes alterations in cell shape and volume and it is associated with cellular rigidity, hemolysis, senescence, and apoptosis [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

Oral Administration ofGanoderma lucidumto Lead-Exposed Rats Protects Erythrocytes against Hemolysis: Implicates to Anti-Anemia

Hossain

Bhowmick

Islam

et al. 2015

Evidence-Based Complementary and Alternative Medicine

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We studied the effect of chronic oral exposure to lead acetate (PbA) on the sensitivity of RBC to hemolysis and whether the sensitivity could be decreased by feeding the rats with extract of medicinal mushroom Ganoderma lucidum. Three groups of rats, control, PbA-exposed, and G. lucidum (Gl)+PbA, were used. PbA (3 mM) was administered via drinking water and G. lucidum extract by gavage at 300 mg/Kg BW/day for 12 weeks. Afterwards, the rats were killed and washed RBCs were subjected to hemolysis in the presence of Fenton's reagents. Hemolysis was determined by estimating the amount of released hemoglobin. The levels of lipid peroxide (LPO) and GSH were determined from RBC membranes and whole RBCs, respectively. The levels of TNFα and LPO also were determined from hepatic tissues. The RBCs of PbA-exposed rats displayed significantly higher sensitivity to hemolysis than those of the Gl+PbA rats. The levels of LPO increased and GSH decreased in the RBCs, with concomitant increases in the levels of hepatic TNFα and LPO in the PbA-exposed rats. The degree of hemolysis was significantly low in the RBCs of Gl+PbA rats, concurrently with amelioration of hepatic parameters. Finally, the study suggests that PbA-induced-hemolysis and related oxidative-toxicity might be minimized by consumption of G. lucidum.

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Section: Discussionmentioning

confidence: 99%

Oral Administration ofGanoderma lucidumto Lead-Exposed Rats Protects Erythrocytes against Hemolysis: Implicates to Anti-Anemia

Hossain

Bhowmick

Islam

et al. 2015

Evidence-Based Complementary and Alternative Medicine

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“…1, and the rate constants for SO 4 2- uptake are shown in Table 1. 5 µM DIDS was chosen to ensure total inhibition of the Band 3 protein [27,28]. NEM induces cell shrinkage and alterations in the redox state, as well as increases the phosphorylation of Band 3 at tyrosine residues [29].…”

Section: Resultsmentioning

confidence: 99%

Sulphate and Chloride-Dependent Potassium Transport in Human Erythrocytes are Affected by Crude Venom from Nematocysts of the JellyfishPelagia noctiluca

Morabito

Marino

Romano

et al. 2013

Cell Physiol Biochem

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Background: It has been reported that biologically active compounds extracted from Cnidaria venom may induce damage by oxidative stress. Erythrocytes are constantly exposed to oxidative stresses, which can contribute to sulphydril (SH-) group oxidation and cell membrane deformability accompanied with activation of K-Cl co-transport and inhibition of anion transport. In this regard, Band 3 protein is responsible for mediating the electroneutral exchange of chloride (Cl^-) for bicarbonate (HCO₃^-), particularly in erythrocytes, where it is the most abundant membrane protein. The aim of this study was to elucidate the effect of crude venom extracted from Pelagia noctiluca nematocysts on Band 3 -mediated anion transport in human erythrocytes. Methods: Erythrocytes were tested for SO₄^2- uptake, K⁺ efflux, glutathione (GSH) levels and concentration of SH- groups. Results: The rate constant of SO₄^2- uptake decreased progressively to 58% of control with increasing venom doses, and showed a 28% decrease after 2 mM NEM treatment. These effects can be explained by oxidative stress, which was reflected by decreased GSH levels in venom-treated erythrocytes. Hence, the decreased efficiency of anion transport may be due to changes in Band 3 structure caused by SH-group oxidation and reduced GSH concentration. In addition, an increased Cl^--dependent K⁺ efflux was observed in venom-treated erythrocytes. Conclusion: Our results suggest that crude venom from Pelagia noctiluca alters cell membrane transport in human erythrocytes.

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“…Lead may also cause deficiency of the enzyme G-6PD (glucose-6 phosphate dehydrogenase) that may inhibit erythrocyte maturation in the bone marrow. (3,4) Gugliotta et al (24) investigated the effect of lead on the proteins in the erythrocyte membrane. Their results showed a decreased erythrocyte count, with membrane permeability playing an important role in the decrease.…”

Section: Discussionmentioning

confidence: 99%

“…In the experimental laboratory study conducted by Gugliotta, (24) heparinized blood with hematocrit concentration of 3% was incubated for 1 hour at 25° C in a medium containing increasing concentrations of lead chloride (0, 0.3, 0.5 and 1 µM). The results of the study showed an increase in erythrocyte permeability with consequent cellular damage and death, particularly at high lead concentrations, changes in morphology and membrane structure of the red blood cell, and decreased erythrocyte ATP concentrations.…”

Section: Discussionmentioning

confidence: 99%

Aqueous IR Bagendit rice leaf extract decreases reticulocyte count in lead-exposed rats

2018

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BACKGROUNDLead acetate may inhibit the enzyme aminolevulinate dehydratase (ALAD) resulting in decreased heme synthesis (and consequently in anemia) but in increased number of reticulocyte cells. IR Bagendit paddy leaf water extract has a high metallothionein protein content which acts to bind to lead. The study objective was to determine whether aqueous IR Bagendit rice leaf extract dosage variations prior to lead exposure decreases reticulocyte count in lead-exposed rats.

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Effects of lead chloride on human erythrocyte membranes and on kinetic anion sulphate and glutathione concentrations

Cited by 10 publications

References 41 publications

Oral Administration ofGanoderma lucidumto Lead-Exposed Rats Protects Erythrocytes against Hemolysis: Implicates to Anti-Anemia

Oral Administration ofGanoderma lucidumto Lead-Exposed Rats Protects Erythrocytes against Hemolysis: Implicates to Anti-Anemia

Sulphate and Chloride-Dependent Potassium Transport in Human Erythrocytes are Affected by Crude Venom from Nematocysts of the JellyfishPelagia noctiluca

Aqueous IR Bagendit rice leaf extract decreases reticulocyte count in lead-exposed rats

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