Effects of Linalyl Acetate on Thymic Stromal Lymphopoietin Production in Mast Cells

Abstract

Thymic stromal lymphopoietin (TSLP) is an important factor responsible for the pathogenesis of allergic diseases, such as atopic dermatitis and asthma. Because linalyl acetate (LA) possesses a wide range of pharmacological properties, being antispasmodic, anti-inflammatory, and anti-hyperpigmentation, we hypothesized that LA could inhibit TSLP. Therefore, enzyme-linked immunosorbent assay, quantitative polymerase chain reaction, caspase-1 assay, Western blot analysis, fluorescent analyses of the intracellular calcium levels, and the phorbol myristate acetate (PMA)-induced edema model were used to investigate how LA inhibits the production of TSLP in HMC-1 cells. LA reduced the production and mRNA expression of TSLP in HMC-1 cells. LA also inhibited the activation of nuclear factor-κB and degradation of IκBα. PMA plus A23187 stimulation up-regulated caspase-1 activity in HMC-1 cells; however, this up-regulated caspase-1 activity was down-regulated by LA. Finally, LA decreased intracellular calcium levels in HMC-1 cells as well as PMA-induced ear swelling responses in mice. Taken together, these results suggest that LA would be beneficial to treatment of atopic and inflammatory diseases by reducing TSLP.

Use of Physcion to Improve Atopic Dermatitis-Like Skin Lesions through Blocking of Thymic Stromal Lymphopoietin

“…The crosslinking of FcεRI-bound IgE with multivalent antigen initiates activation of mast cells, then mast cell activation results in protein kinase C (PKC) activation as well as intracellular calcium elevation [29]. To produce similar conditions, we selected PMA to activate PKC and calcium ionophore to increase intracellular calcium level in this study.…”

p-coumaric acid, an active ingredient of Panax ginseng, ameliolates atopic dermatitis-like skin lesions through inhibition of thymic stromal lymphopoietin in mice

“…In general, protein kinase C (PKC) activation and intracellular calcium upregulation result from mast cell activation by binding with antigens [ 33 ]. To reenact this circumstance, we used PMA for PKC activation and calcium ionophore for intracellular calcium elevation in HMC-1 cells.…”

Effects of Resveratrol on Thymic Stromal Lymphopoietin Expression in Mast Cells

“…In general, mast cell activation results from the binding of IgE receptors with multivalent antigens. Activation of protein kinase C (PKC), as well as increment of intracellular calcium are shown in activated mast cells [ 37 ]. To make a similar condition, PMA was used to activate PKC, and calcium ionophore was used to increase intracellular calcium in this experiment.…”