Effects of lipopolysaccharide on the histomorphology and expression of toll-like receptor 4 in the chicken trachea and lung

Ansari, Abdur Rahman; Ge, Xin; Huang, Haibo; Huang, Xiang; Zhao, Xing; Peng, Kaiman; Zhong, Juming; Liu, Huazhen

doi:10.1080/03079457.2016.1168923

Cited by 20 publications

(11 citation statements)

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“…Healthy one-day-old commercial Cobb strain (genetically Cobb 500) broiler chicks were purchased from Zhengda chicken breeding company (Wuhan, China) and chicks with uniform body weight were selected and provided with commercial chick-starter feed and water ad libitum along with supplementary heating without any vaccinations [ 19 ]. All the birds were intraperitoneally (i.p.)…”

Section: Methodsmentioning

confidence: 99%

“…All the birds were intraperitoneally (i.p.) injected at the same peritoneal location by lifting the skin over mid-abdominal line, immediately anterior to the pubic bones with LPS derived from Salmonella enterica serovar Typhimurium (STm) (L7261; Sigma-Aldrich, St. Louis, MO, USA) at 50 mg/kg of body weight in 0.5 mL avian saline solution (0.75% NaCl) [ 19 ]. Birds in the control group were exposed to mock infection with 0.5 mL avian saline solution only.…”

Section: Methodsmentioning

confidence: 99%

“…The tissue sections were immunostained by following the same steps as described previously [ 19 , 20 ]. In brief, serial liver tissue sections were deparaffinized twice in xylene and rehydrated in a graded series of ethanol.…”

Section: Methodsmentioning

confidence: 99%

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Lipopolysaccharide mediates immuno-pathological alterations in young chicken liver through TLR4 signaling

et al. 2017

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BackgroundLipopolysaccharide (LPS) induces acute liver injury and the complex mechanisms include the activation of toll like receptor 4 (TLR4) signaling pathway in many species. However, immuno-pathological changes during TLR4 signaling under LPS stress in acute liver injury is poorly understood in avian species. The present investigation was therefore carried out to evaluate these alterations in TLR4 signaling pathway during acute liver injury in young chickens.ResultsAfter intraperitoneal injection of LPS or saline, liver samples were harvested at 0, 2, 6, 12, 24, 36, 72 and 120 h (n = 6 at each time point) and the microstructures were analyzed by hematoxylin and eosin (H&E) staining. Alanine aminotransferase (ALT) and caspase-3 enzyme activity was assessed by enzyme-linked immunosorbent assay (ELISA). Proliferative cell nuclear antigen (PCNA), single stranded DNA (ssDNA) and TLR4 protein expressions were determined by immunohistochemistry. Gene expressions of PCNA, caspase-3, caspase-8, TLR4 and its downstream molecules were analyzed by quantitative polymerase chain reaction (qPCR). LPS injection induced significantly higher ALT activity, severe fatty degeneration, necrotic symptoms, ballooning degeneration, congestion, enhanced inflammatory cell infiltration in liver sinusoids, decreased proliferation, increased apoptosis and significant up-regulation in TLR4 and its downstream molecules (MyD88, NF-κB, TNF-α, IL-1β and TGF-β) expression at different time points.ConclusionsThis study indicated that TLR4 signaling and its downstream molecules along with certain cytokines play a key role in acute liver injury in young chickens. Hence, our findings provided novel information about the histopathological, proliferative and apoptotic alterations along with changes in ALT and caspase-3 activities associated with acute liver injury induced by Salmonella LPS in avian species.Electronic supplementary materialThe online version of this article (doi:10.1186/s12865-017-0199-7) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Lipopolysaccharide mediates immuno-pathological alterations in young chicken liver through TLR4 signaling

et al. 2017

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“…Some special features of avian lungs, such as a single basal lamina and a thin squamous epithelium layer at the blood-gas interface, predispose their respiratory systems to bacterial pathogens and possibly LPS-induced acute lung injury. 44 In some previous studies, E. coli- derived LPS were used to induce the model of acute lung injury in chickens. 37 , 44 , 45 In the current experiment, LPS treatment in the positive control group clearly stimulated a diffuse inflammatory response, severe hemorrhage, and thicker interatrial septum, which resulted in higher ALI scores in the positive control group as compared with those in the negative control group.…”

Section: Discussionmentioning

confidence: 99%

<p>Comparative Effects of Dexamethasone and Meloxicam on Magnitude of the Acute Inflammatory Response Induced by <em>Escherichia coli</em> Lipopolysaccharide in Broiler Chickens</p>

Tavakoli¹,

Abdi-Hachesoo²,

Nazifi³

et al. 2020

JIR

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Purpose: Dexamethasone has been widely used to treat acute inflammatory diseases and endotoxic shocks in animal models. Meloxicam is one of the most commonly used antiinflammatory agents in avian species. However, little is known about the effects of dexamethasone and meloxicam on lipopolysaccharide (LPS)-induced acute inflammatory response in birds. In the present study, LPS-challenged broiler chickens were used to investigate the comparative protective effects of meloxicam and dexamethasone on LPSinduced acute inflammatory responses. Methods: Lipopolysaccharide (LPS)-induced acute lung injury (ALI) histopathological scores, selected serum acute phase reactants, inflammatory mediators, and gangliosides were evaluated in broiler chickens inoculated with E. coli LPS and simultaneously treated with two doses of meloxicam (0.5 and 2 mg/kg BW) and dexamethasone (2 and 4 mg/kg BW). Results: LPS-induced ALI scores were not significantly different between the meloxicamtreated, dexamethasone-treated, and untreated positive control groups at 4 hours after LPS inoculation. Interleukin-6 concentrations were also statistically the same among the positive control, dexamethasone-treated, and meloxicam-treated groups at 3 and 12 hours after LPS inoculation. However, these anti-inflammatory drugs reduced adenosine deaminase, ceruloplasmin, lipid-bound sialic acid, protein-bound sialic acid, and total sialic acid in LPS-inoculated broiler chickens at 12, 24, and 48 hours after LPS inoculation in a drug-and dose-dependent manner. Ovotransferrin concentrations were not significantly different between positive control and treatment groups at 12 hours after LPS inoculation. However, twenty-four hours after LPS inoculation, all the treated groups, except the one treated with 0.5 mg/kg meloxicam, showed significantly lower concentrations of ovotransferrin as compared with the positive control group. Conclusion: Our results showed that dexamethasone was more effective than meloxicam in inhibiting the LPS-induced response in broiler chickens by diminishing the serum levels of adenosine deaminase, ceruloplasmin, and gangliosides.

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“…To test the distribution and protein expression of P-AMPK, immunohistochemical staining was performed following the same steps as described in earlier studies [63,64]. Brie y, the tissue sections were depara nized twice in xylene and rehydrated in a graded series of ethanol.…”

Section: Immunohistochemical Stainingmentioning

confidence: 99%

Cecal microbiota affects chicken growth performance by regulating fat metabolism

Zhang¹,

Y²,

Ansari³

et al. 2020

Preprint

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Background A growing body of evidences suggest critical role of the chicken gut microbiota in growth performance and fat metabolism. However, the underlying mechanism by which the gut microbiota affects chicken growth performance by regulating fat metabolism remains unclear. The purpose of current study was to compare cecal microbial communities between high and low body weight chickens and verify the correlation between fat metabolism and gut microbiota. Results Seven-week-old male and female chickens with significantly different body weight were used in the present study. 16S rRNA gene sequencing was used to reveal the cecal microbial community. Fat metabolism levels were compared between high and low body weight chickens. Spearman correlation analysis was used to analyze the relationship between the cecal microbiota and fat metabolism. Transferring fecal microbiota from adult chickens with high body weight into one-day-old chickens was examined by oral administration to verify gut microbiota effects on chicken growth through regulation of fat metabolism. There were significant differences in body weight, chest and leg muscle indexes as well as in cross-sectional area of muscle cells, suggesting different growth performance between high and low body weight chickens. By comparing the relative abundance of gut microbes in the cecal content in high and low body weight chickens, we found that Microbacterium and Sphingomonas were more abundant in high body weight chickens and Slackia was more abundant in low body weight chickens. The fat metabolism level was markedly different in serum, liver, abdominal adipose, chest and leg muscles between high and low body weight chickens. Spearman correlation analysis showed a positive correlation between fat metabolism and the relative abundance of Microbacterium and Sphingomonas and a negative correlation between fat metabolism and the abundance of Slackia. Hence, transferring fecal microbiota, instead of saline, from adult chickens with high body weight into one-day-old chickens improved growth performance and fat metabolism in liver. Conclusions These results suggested that cecal microbiota could affect chicken growth performance by regulating fat metabolism.

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Effects of lipopolysaccharide on the histomorphology and expression of toll-like receptor 4 in the chicken trachea and lung

Cited by 20 publications

References 29 publications

Lipopolysaccharide mediates immuno-pathological alterations in young chicken liver through TLR4 signaling

Lipopolysaccharide mediates immuno-pathological alterations in young chicken liver through TLR4 signaling

<p>Comparative Effects of Dexamethasone and Meloxicam on Magnitude of the Acute Inflammatory Response Induced by <em>Escherichia coli</em> Lipopolysaccharide in Broiler Chickens</p>

Cecal microbiota affects chicken growth performance by regulating fat metabolism

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