Effects of Liuwei Dihuang decoction on ion channels and synaptic transmission in cultured hippocampal neuron of rat

TREK1 channel blockade induces an antidepressant-like response synergizing with 5-HT1A receptor signaling

“…The pipette and bath solution for I K , I Na and I Ca referred to the previously description (Mazella et al, 2010;Li et al, 2010;Yang et al, 2006) respectively, and K 2 P currents were recorded in the presence of 10 mM tetraethylammonium bromide (TEA-Br) after stimulation by 10 mM arachidonic acid (AA) (Mazella et al, 2010). I K and I Na were elicited by depolarizing the neurons from À 40 to +30 mV in 5 mV increments for 200 ms (I K ) and 10 ms (I Na ).…”

“…I K and I Na were elicited by depolarizing the neurons from À 40 to +30 mV in 5 mV increments for 200 ms (I K ) and 10 ms (I Na ). The parameter of I Ca referred to the previously description (Yang et al, 2006). PClampex10.2 software was used to analyze the currents (I K + , I Ca2 + ) recorded in whole cell mode at 0 mV (I Na + : at À25 mV).…”

Rehmannia glutinosa: Review of botany, chemistry and pharmacology

“…Liuwei Dihuang decoction (LW) is a famous TCM prescription and consists of six herbs including Rehmannia glutinosa Libosch., etc. LW-containing serum (LWCS) possessed the effect of modulating or improving neuronal and synaptic function by whole-cell patch clamp recording technique (Yang et al, 2006).…”

Protective effect of Shenqi-wan and its fractions on N-methyl-D-aspartate-induced excitotoxicity in rat hippocampus

“…Shin et al (2003) reported that Shenqi-wan has neuroprotective effect on H 2 O 2 -induced damage in HiB5 cell line and they also showed that this neuroprotective effect of Shenqiwan on exitotoxicity was induced by suppressing glutamate-activated and NMDA-activated ion currents in rat hippocampal CA1 neurons. Also Yang et al (2006) reported that Liuweidihuang decoction, similar to Shenqi-wan, suppressed K + and Ca 2+ ion currents in cultured rat hippocampal neurons.…”