Effects of local anesthetics on bacterial cells

Silva, M T; Sousa, Joana; Polonia, J.; Macedo, M. Paula

doi:10.1128/jb.137.1.461-468.1979

Cited by 66 publications

(28 citation statements)

References 28 publications

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“…The values are presented as the percentage of K + leaked in comparison to that from cells boiled for 30 minutes. 9,10 Viability counts and the percentage of cells stained by PI were also determined in the suspensions used for the K + leakage assays. The leakage of K + was also analyzed for Candida cells treated with 20 mM sodium azide, or with 2µg/mL of amphotericin B, for 10 minutes.…”

Section: Studies Of Membrane Damagingmentioning

confidence: 99%

Antifungal Activity of Local Anesthetics AgainstCandida Species

Pina-Vaz

Rodrigues

Sansonetty³

et al. 2000

Infectious Diseases in Obstetrics and Gynecology

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Section: Studies Of Membrane Damagingmentioning

confidence: 99%

Antifungal Activity of Local Anesthetics AgainstCandida Species

Pina-Vaz

Rodrigues

Sansonetty³

et al. 2000

Infectious Diseases in Obstetrics and Gynecology

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“…Clinically, it has been well known in dentistry that local anesthetic agents rarely cause local infection at the injection site without disinfection treatment. Local anesthetics such as procaine, dibucaine, and tetracaine are known to bind specifically to the cell membranes of bacteria as well as mammalian cells, and to alter the structure and fluidity of the membranes, to disrupt membrane potential and to inhibit membrane-bound respiratory function at relatively higher concentrations (2,22,26). Other physiological effects on bacteria have also been observed.…”

mentioning

confidence: 99%

Lidocaine Hydrochloride and Acetylsalicylate Kill Bacteria by Disrupting the Bacterial Membrane Potential in Different Ways

Ohsuka

Ohta

Masuda

et al. 1994

Microbiology and Immunology

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Lidocaine hydrochloride (LH), a local anesthetic, and acetylsalicylate (AcSAL), show antibacterial activity for both gram-negative and gram-positive bacteria. Kinetic studies indicated that antibacterial activity of LH was different from that of AcSAL. A subinhibitory concentration of LH and AcSAL enhanced the sensitivity of Escherichia coli, Salmonella typhimurium, and Pseudomonas aeruginosa to novobiocin and nalidixic acid. The synergistic effect of AcSAL with novobiocin and nalidixic acid was higher than that of LH. The effect of both drugs on the membrane potential of inner membrane was also studied using inverted membrane vesicles of bacteria. Both LH and AcSAL depolarized the membrane potential after the vesicles were energized with nicotinamide adenine dinucleotide. However, unlike AcSAL, pre-treatment of vesicles with LH had no effect on the generation of membrane potential. These results suggest that depolarization of the cytoplasmic membrane, preceded by the permeabilization of the outer membrane for gram-negative bacteria, is associated with antibacterial activity of LH and AcSAL. The difference in actions of LH and AcSAL was discussed.

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“…1,3,4 Bacterial cell membrane disruption, alteration in deoxyribonucleic acid synthesis, lysis of protoplasts, permeability and ultrastructural changes, and inhibition of membrane-bound enzymatic activities are proposed mechanisms for inhibitory effects of local anesthetics on bacteria. 5,6 Until recently, investigations evaluating antimicrobial effects of local anesthetics have been mainly in vitro studies.…”

Section: Discussionmentioning

confidence: 99%