Effects of Locally Administered Tiludronic Acid on Experimental Periodontitis in Rats

Abstract

It can be concluded that locally administered TIL solution (1 mg/kg body weight) reduced alveolar bone loss in experimental periodontitis and the dosage of TIL may influence its anti-inflammatory and antiresorptive properties.

Benefits of Bifidobacterium animalis subsp. lactis Probiotic in Experimental Periodontitis

“…For volumetric measurements, a volume of interest (VOI‐prismatic section) was outlined from the apexes of all roots of M1 up to the roof of the furcation of M1, touching the roots surfaces, 21 in all images of the coronal dataset, using appropriate software ****. The following parameters were analyzed: 1) BV: percentage of VOI filled with bone tissue; 2) bone porosity (BP): percentage of porosities present in the bone tissue within the VOI; 3) trabecular number (Tb.N): number (mm ‐1 ) of bone trabeculae present within the VOI; 4) trabecular separation (Tb.Sp): total of spaces (mm) present among bone trabeculae within the VOI.…”

Effects of the probiotic Bifidobacterium animalis subsp. lactis on the non-surgical treatment of periodontitis. A histomorphometric, microtomographic and immunohistochemical study in rats

“…Immunohistochemical processing was performed through the indirect immunoperoxidase method, as described elsewhere [21]. The histological slides containing samples from all animals were incubated with one of the following primary antibodies: rabbit anti-interleukin (IL)-1β (1:100-Rabbit anti-IL-1β–SC 7884, Santa Cruz Biotechnology, Santa Cruz, CA, USA), goat anti-cytokine-induced neutrophil chemoattractant–CINC (1:100-Goat anti-CINC1–ab 10365, abcam ® , Cambridge, MA, USA), goat anti-IL-10 (1:100-Goat anti-IL-10–SC 1783, Santa Cruz Biotechnology), rabbit anti-transforming growth factor (TGF)-β1 (1:200-Rabbit anti-TGF-β1–SC 146, Santa Cruz Biotechnology) or goat anti-tartrate-resistant acid phosphatase–TRAP (1:100-Goat anti-TRAP–SC 30833, Santa Cruz Biotechnology).…”

Effects of local administration of tiludronic acid on experimental periodontitis in diabetic rats

“…Immunohistochemical processing was performed through the indirect immunoperoxidase method, as described previously . Histologic slides containing samples from all the experimental groups were divided into three batches, and each batch was incubated with one of the following primary antibodies: 1) goat anti‐tartrate‐resistant acid phosphatase (TRAP); 2) goat anti–receptor activator of nuclear factor‐κB ligand (RANKL); 3) goat antiosteoprotegerin (OPG); or 4) rabbit anti–cleaved caspase 3 (CC3)…”

“…A quantitative immunolabeling analysis was performed for TRAP and CC3. TRAP‐positive cells were distinguished as TRAP‐positive multinucleated cells (mature osteoclasts) or TRAP‐positive mononuclear cells . Only osteocytes CC3‐positive were quantified.…”

“…In vitro studies demonstrated that this bisphosphonate also presents anti‐inflammatory actions, as it can dose‐dependently inhibit interleukin (IL)‐6 synthesis by osteoblasts and the secretion of IL‐1β, IL‐6, nitric oxide (NO), and tumor necrosis factor (TNF)‐α by activated macrophages . Recently, the author group verified that local administrations of TIL in gingival tissues decreased expression of osteoclasts and alveolar bone loss (ABL) in experimental periodontitis (EP) in non‐diabetic rats . The author group also observed that this therapy reduced expression of some inflammatory mediators, such as TNF‐α, IL‐1β, and matrix metalloproteinase (MMP)‐8 (unpublished observations), which may be altered in the periodontium of patients with DM …”