Effects of low crystalline carbonate apatite on proliferation and osteoblastic differentiation of human bone marrow cells

Nagai, Hirokazu; Kobayashi-Fujioka, Masako; Fujisawa, Kenji; Ohe, Go; Takamaru, Natsumi; Hara, Kanae; Uchida, Daisuke; Tamatani, Tetsuya; Ishikawa, Kunio; Miyamoto, Youji

doi:10.1007/s10856-015-5431-5

Cited by 89 publications

(81 citation statements)

References 37 publications

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“…Because of the absence and presence of osteoclastic resorption, sintered HAp is not replaced by new bone, whereas CO 3 Ap block is. CO 3 Ap can upregulate differentiation of osteoblasts more than HAp [15]. Moreover, CO 3 Ap has demonstrated higher osteoconductivity than sintered HAp.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of Carbonate Apatite Block through a Dissolution–Precipitation Reaction Using Calcium Hydrogen Phosphate Dihydrate Block as a Precursor

et al. 2017

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Carbonate apatite (CO3Ap) block, which is a bone replacement used to repair defects, was fabricated through a dissolution–precipitation reaction using a calcium hydrogen phosphate dihydrate (DCPD) block as a precursor. When the DCPD block was immersed in NaHCO3 or Na2CO3 solution at 80 °C, DCPD converted to CO3Ap within 3 days. β-Tricalcium phosphate was formed as an intermediate phase, and it was completely converted to CO3Ap within 2 weeks when the DCPD block was immersed in Na2CO3 solution. Although the crystal structures of the DCPD and CO3Ap blocks were different, the macroscopic structure was maintained during the compositional transformation through the dissolution–precipitation reaction. CO3Ap block fabricated in NaHCO3 or Na2CO3 solution contained 12.9 and 15.8 wt % carbonate, respectively. The diametral tensile strength of the CO3Ap block was 2 MPa, and the porosity was approximately 57% regardless of the carbonate solution. DCPD is a useful precursor for the fabrication of CO3Ap block.

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Section: Introductionmentioning

confidence: 99%

Fabrication of Carbonate Apatite Block through a Dissolution–Precipitation Reaction Using Calcium Hydrogen Phosphate Dihydrate Block as a Precursor

et al. 2017

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“…2, can be regarded as a stress reaction and is not necessarily a sign of stem cell differentiation, since differentiation of embryonic stem cells to osteoblasts is expected to take 7 days rather than 24 h. 38 However, stem cells can be a significant source of HA at the bone-implant interface during bone healing, and HA itself seems to promote the differentiation of stem cells. 39 …”

Section: Resultsmentioning

confidence: 99%

Formation of hydroxyapatite on titanium implants in vivo precedes bone-formation during healing

et al. 2017

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The bone material interface has been an area of intense study over many decades, where studies of the healing process ranging from simple mineral deposition in vitro to actual healing in vivo have given important clues to the importance of calcium minerals in the bone/implant interface. Here, the authors use a combination of in vitro cell culture methods and in vivo implantation to study how the role of the spontaneously formed hydroxyapatite layer on Ti-implants for the in vivo-healing into the bone tissue of rat tibia. Initial experiments were made in reduced systems by incubation of TiO 2 in cell culture medium and analysis by time of flight secondary ion mass spectrometry (ToF-SIMS) and energy-dispersive x-ray spectroscopy followed by subsequent exposure of human embryological stem cells analyzed by von Kossa staining and environmental scanning electron microsopy. In vivo studies of the bone-material interface was analyzed by ToF-SIMS depth profiling using both C 60 þ ions as well as a gas cluster ion source beam, Ar 1500 þ as sputter source. The low ion yield of the Ar 1500 þ for inorganics allowed the inorganic/organic interface of the implant to be studied avoiding the erosion of the inorganic materials caused by the conventional C 60 þ beam.

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“…osteoblastic markers of differentiation, such as ALP, type I collagen, osteocalcin, and osteopontin [19]. Table 2 shows that there was a significant difference between group B and group C (p<0.05) on day 7 and 14 after debonding.…”

Section: -4mentioning

confidence: 96%

Effect of carbonated hydroxyapatite incorporated advanced platelet rich fibrin intrasulcular injection on the alkaline phosphatase level during orthodontic relapse

Alhasyimi¹,

Pudyani²,

Asmara³

et al. 2018

AIP Conference Proceedings

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Effects of low crystalline carbonate apatite on proliferation and osteoblastic differentiation of human bone marrow cells

Cited by 89 publications

References 37 publications

Fabrication of Carbonate Apatite Block through a Dissolution–Precipitation Reaction Using Calcium Hydrogen Phosphate Dihydrate Block as a Precursor

Fabrication of Carbonate Apatite Block through a Dissolution–Precipitation Reaction Using Calcium Hydrogen Phosphate Dihydrate Block as a Precursor

Formation of hydroxyapatite on titanium implants in vivo precedes bone-formation during healing

Effect of carbonated hydroxyapatite incorporated advanced platelet rich fibrin intrasulcular injection on the alkaline phosphatase level during orthodontic relapse

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