Effects of low dose acrylamide on the rat reproductive organs structure, fertility and gene integrity

Alkarim, Saleh; ElAssouli, Sufyan; Ali, Soad Shaker; Ayuob, Nasra Naeim; ElAssouli, Zaki

doi:10.1016/j.apjr.2015.05.001

Cited by 27 publications

(19 citation statements)

References 25 publications

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“…The results of many assays made by various laboratories are consistent in showing that AA is not a mutagen in Salmonella Typhimurium tested strains at concentrations up to 5 mg/plate, with or without metabolic activation [3]. However, three epoxide analogs of acrylamide, e.g., glycidamide, have been reported to be mutagenic in Salmonella strains ± S9 activation [11,13]. Tsuda et al [14] reported that AA did not induce any gene mutations in Salmonella/microsome test systems (TA98, TA100, TA1535, TA1537) and in Escherichia coli/microsome assays (WP2 uvrA − ) up to a dose of 50 mg AA/plate, but acrylamide did show a strong positive response in a Bacillus subtilis spore-rec assay (induced DNA damage) at 10-50 mg/disc.…”

Section: Introductionmentioning

confidence: 54%

“…It exerts neurotoxic activity [5][6][7], and many studies have proved that AA also has genotoxic, cytotoxic, and carcinogenic impacts on the human organism [6,[8][9][10]. However, due to the fact that acrylamide does not exert a mutagenic effect in bacterial cells [3,11], it has been agreed that its carcinogenic activity is related to glycidamide (GA)-an acrylamide metabolite formed in mammalian cells. The mutagenic and genotoxic effects of GA have already been confirmed in various in vitro and in vivo studies, showing that this AA metabolite can induce the formation of DNA adducts, resulting in mutagenesis and the development of cancers [6,8,9,12].…”

Section: Introductionmentioning

confidence: 99%

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Is Acrylamide as Harmful as We Think? A New Look at the Impact of Acrylamide on the Viability of Beneficial Intestinal Bacteria of the Genus Lactobacillus

2020

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The impact of acrylamide (AA) on microorganisms is still not clearly understood as AA has not induced mutations in bacteria, but its epoxide analog has been reported to be mutagenic in Salmonella strains. The aim of the study was to evaluate whether AA could influence the growth and viability of beneficial intestinal bacteria. The impact of AA at concentrations of 0–100 µg/mL on lactic acid bacteria (LAB) was examined. Bacterial growth was evaluated by the culture method, while the percentage of alive, injured, and dead bacteria was assessed by flow cytometry after 24 h and 48 h of incubation. We demonstrated that acrylamide could influence the viability of the LAB, but its impact depended on both the AA concentration and the bacterial species. The viability of probiotic strain Lactobacillus acidophilus LA-5 increased while that of Lactobacillus plantarum decreased; Lactobacillus brevis was less sensitive. Moreover, AA influenced the morphology of L. plantarum, probably by blocking cell separation during division. We concluded that acrylamide present in food could modulate the viability of LAB and, therefore, could influence their activity in food products or, after colonization, in the human intestine.

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Section: Introductionmentioning

confidence: 54%

Section: Introductionmentioning

confidence: 99%

Is Acrylamide as Harmful as We Think? A New Look at the Impact of Acrylamide on the Viability of Beneficial Intestinal Bacteria of the Genus Lactobacillus

2020

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“…[41] Another in vivo study showed that AA increased DNA damage and genotoxicity in the male and female reproductive systems. [42] An in vitro study conducted by Wei et al [36] demonstrated that GA elevated tail DNA (%), tail length and Olive tail moment in R2C Leydig cells. Furthermore, it has been shown that GA stimulates genotoxicity by increasing the frequencies of micronucleus.…”

Section: Micronucleus Testmentioning

confidence: 99%

Vitamin C inhibits glycidamide‐induced genotoxicity and apoptosis in Sertoli cells

Yılmaz

Yildizbayrak

Aydın

2020

J Biochem & Molecular Tox

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Exposure to the food contaminant acrylamide and its reactive epoxide metabolite glycidamide (GA) induces reactive oxygen species (ROS)‐mediated oxidative stress and subsequent cellular death. Recent studies have revealed that the toxic effects of acrylamide may be due to GA, especially on male reproductive system cells. In this regard, it is important to determine the effects of GA on Sertoli cells, which are essential cells for the male reproductive system. Antioxidants should be consumed in sufficient quantities to minimise the effects of environmental pollutants. This study aimed to determine the direct toxic effects of GA and protective effects of vitamin C (VitC) against GA‐induced damage in Sertoli cells by measuring cell viability, cytotoxicity, lipid peroxidation, ROS, antioxidant enzyme levels, apoptosis and DNA damage. Sertoli cells were exposed to GA for 24 hours at four different concentrations (ranging between 1 and 1000 μM) and in addition to these GA concentrations to VitC (50 μM). The results of cytotoxicity markers, such as cell viability and lactate dehydrogenase (LDH) showed that GA significantly reduced cell viability and increased LDH levels. We also found that GA induced overproduction of intracellular ROS, increased lipid peroxidation in cellular membrane and triggered cell apoptosis and genotoxicity. In addition, VitC supplementation ameliorated the adverse effects of GA on Sertoli cells. Consequently, these findings suggest that GA may damage the cell function in Sertoli cells, depending on the concentration. Additionally, it was evidenced that VitC has an ameliorative effect on toxicity caused by GA.

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“…In addition, ALKarim et al . [ 35 ] determined that long-term exposure to a 60 μg/kg/day dose of ACR was associated with cystic ovarian changes and degenerative changes of the zona pellucida, granulosa cells and oocytes in post-weaning Sprague Dawley rats. In light of these data, we conclude that short-term or single-dose exposures to ACR are not solely responsible for toxicity.…”

Section: Discussionmentioning

confidence: 99%

In Vivo acrylamide exposure may cause severe toxicity to mouse oocytes through its metabolite glycidamide

et al. 2017

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High acrylamide (ACR) content in heat-processed carbohydrate-rich foods, as well as roasted products such as coffee, almonds etc., has been found to be as a risk factor for carcinogenicity and genotoxicity by The World Health Organization. Glycidamide (GLY), the epoxide metabolite of ACR, is processed by the cytochrome P-450 enzyme system and has also been found to be a genotoxic agent. The aim of this study was to determine whether ACR and/or GLY have any detrimental effect on the meiotic cell division of oocytes. For this purpose, germinal vesicle-stage mouse oocytes were treated with 0, 100, 500, or 1000 μM ACR or 0, 25, or 250 μM GLY in vitro. In vivo experiments were performed after an intraperitoneal injection of 25 mg/kg/day ACR of female BALB/c mice for 7 days. The majority of in vitro ACR-treated oocytes reached the metaphase-II stage following 18 hours of incubation, which was not significantly different from the control group. Maturation of the oocytes derived from in vivo ACR-treated mice was impaired significantly. Oocytes, reaching the M-II stage in the in vivo ACR-treated group, were characterized by a decrease in meiotic spindle mass and an increase in chromosomal disruption. In vitro GLY treatment resulted in the degeneration of all oocytes, indicating that ACR toxicity on female germ cells may occur through its metabolite, GLY. Thus, ACR exposure must be considered, together with its metabolite GLY, when female fertility is concerned.

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Effects of low dose acrylamide on the rat reproductive organs structure, fertility and gene integrity

Cited by 27 publications

References 25 publications

Is Acrylamide as Harmful as We Think? A New Look at the Impact of Acrylamide on the Viability of Beneficial Intestinal Bacteria of the Genus Lactobacillus

Is Acrylamide as Harmful as We Think? A New Look at the Impact of Acrylamide on the Viability of Beneficial Intestinal Bacteria of the Genus Lactobacillus

Vitamin C inhibits glycidamide‐induced genotoxicity and apoptosis in Sertoli cells

In Vivo acrylamide exposure may cause severe toxicity to mouse oocytes through its metabolite glycidamide

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