Effects of low-frequency electrical stimulation on fast and slow muscles of the rat

Kwong, Wing-Hang; Vrbová, Gerta

doi:10.1007/bf00596171

Cited by 49 publications

(16 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Others found in the musculus extensor digitorum longus and musculus tibialis anterior, fast-twitch muscles of the limb, a change of type 2B into type 2A fibres, the last being intermediate fast-twitch fibres with a fast oxidative glycolytic activity which are better adapted to sustain contraction [30]. However, no increase in slow-type fibres were found in fast limb muscles of the rat [31][32][33] or of the rabbit [20] even after a long stimulation period. Pette and Vrbova [30] suggested that stimulation-induced changes in the muscle fibre population may proceed in the following order: type 2B-type 2D-type 2A-type 2C-type 1C-type 1.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Functional and histological effects of intravaginal electrical stimulation on the pelvic muscles: a study in the rat

Wyndaele¹,

Poortmans²

2005

Int Urogynecol J

View full text Add to dashboard Cite

We studied functional and histological effects of electrical stimulation (ES) on pelvic muscles of the rat. With intravaginal electrodes, the musculus pubococcygeus and musculus iliococcygeus in the awake animal were stimulated three times 6 min per day with 5 min of rest in between, 5 days per week, 7 consecutive weeks with a biphasic rectangular symmetrical current of 25 Hz, 400-mus pulse duration, on/off time of 5/10 and with an amplitude of 2-4 mA. A "sham group" received the same handling but no stimulation. Contraction measured with intra-rectal pressure during stimulation increased more in the stimulated than in the sham group, but did not reach statistical significance probably due to low power. The 2A fast fibres increased with 14% in the musculus iliococcygeus and with 6% in the musculus pubococcygeus. Type 1 slow fibres did not change. Increased capillary density was found after stimulation. Repeated intravaginal ES has mainly an influence on the fast fibres in the pelvic muscles. To influence slow fibres, another stimulation program or current parameters would seem necessary.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Kwong and Vrbova [31], when stimulating fast-twitch muscles of the rat with 10 Hz during 8, 10 and 24 h a day for 12 to 41 days, saw a higher capillary density and a more homogenous acid-ATPase staining. The unstimulated muscles displayed different degrees of staining intensity (light, intermediate dark).…”

Section: Discussionmentioning

confidence: 99%

Functional and histological effects of intravaginal electrical stimulation on the pelvic muscles: a study in the rat

Wyndaele¹,

Poortmans²

2005

Int Urogynecol J

View full text Add to dashboard Cite

show abstract

“…In animal experimental muscle models for skeletal muscle hypertrophy, NMES is generally performed with intermittent and high frequencies (more than 60 Hz) [16–19]. On the other hand, when NMES was applied with a lower electrical frequency (~20 Hz) and long activation times, it led to higher oxidative properties and a fast-to-slow conversion of the muscle fiber phenotype [20]. Therefore, animal experimental models with NMES were used to mimic resistance training and/or endurance exercise in order to test the effects of changes in frequency and stimulation time on muscle hypertrophy [21].…”

Section: Introductionmentioning

confidence: 99%

Effect of Intermittent Low-Frequency Electrical Stimulation on the Rat Gastrocnemius Muscle

Tsutaki

Ogasawara

Kobayashi

et al. 2013

BioMed Research International

View full text Add to dashboard Cite

Low-frequency neuromuscular electrical stimulation (NMES) has been used as an endurance exercise model. This study aimed to test whether low-frequency NMES increases the phosphorylation of anabolic signaling molecules and induces skeletal muscle hypertrophy, as seen with high-frequency NMES. Using Sprague-Dawley rats, 1 bout of exercise (with dissection done immediately (Post0) and 3 h (Post3) after exercise) and another 6 sessions of training were performed. All experimental groups consisted of high- and low-frequency stimulation (HFS: 100 Hz; LFS: 10 Hz). Periodic acid-Schiff (PAS) staining was conducted to investigate type II fiber activation, and western blot analysis (WB) was conducted to examine whether NMES leads to anabolic intracellular signaling. At first, we examined the acute effect of exercise. PAS staining revealed that glycogen depletion occurred in both type I and type II fibers. WB results demonstrated that p70S6K phosphorylation was significantly increased by HFS, but there was no significant difference with LFS. In contrast, ERK 1/2 phosphorylation was increased by LFS at Post0. In the 6-session training, the wet weight and myofibrillar protein were significantly increased by both HFS and LFS. In conclusion, LFS has a similar anabolic effect for skeletal muscle hypertrophy as HFS, but the mediating signaling pathway might differ.

show abstract

“…Die tetanic tension (tetanische Spannung) nahm bei intermittierender Stimulation ab der 2. Woche um 12 -13 % zu, die twitch:tetanus ratio stieg an [53,79,96,97,[117][118][119]127,[171][172][173][174][175][176][177]. Auch hier gab es in den ersten beiden Wochen nach Stimulationsbeginn einen vorübergehenden gegenteiligen Effekt [209].…”

unclassified

Electrostimulation in sports and rehabilitation

Kern

2011

Eur J Transl Myol

View full text Add to dashboard Cite

The known effects of Neuromuscular Electrical Stimulation (NMES) in Sports and Physical Medicine and Rehabilitation are presented in a review of literature. Influences of NMES on strength, endurance, neural adaptations, therapy of edema and pain as well as functional improvements will be focused on. Basics of physiology and histology in human muscle tissues are discussed in depth as well as elementary knowledge of NMES derived from animal studies. The central topic of this review is the influence of NMES on human skeletal muscle regarding histological changes, fiber transformation as well as strength and endurance of muscles involved. We optimized NMES characteristics and then conducted a series of investigations with 9 volunteers that had 30 minutes of NMES applied twice daily for 7 weeks. Muscle biopsies were taken from the vastus lateralis portion of quadriceps femoris muscle on both the stimulated and no stimulated leg before and after the 7 week NMES period. Additionally participants performed isometric strength measurements of quadriceps femoris muscle at different knee joint angles before and after the 7 week stimulation period. Biopsies from the no stimulated side showed no significant changes and may therefore serve as proof for the quality of the biopsies and as a standard for comparison. The increment of type IIa fiber volume was 12.3% and of type IIa fiber number 16%, respectively. Volume density of intermyofibrillar mitochondria increased by 22%. Subsarcolemmal mitochondria remained unchanged which contrasts the effects seen in voluntary non-NMES muscle training. Capillary density was augmented by 14.58%. NMES is an appropriate means of adjusting longstanding muscle dysbalance regarding strength, endurance and improving oxidative metabolism in human skeletal muscle. Daily muscle fiber workload (i.e. number of stimuli per day) was found to be the main determinant of fiber transformation.

show abstract

Effects of low-frequency electrical stimulation on fast and slow muscles of the rat

Cited by 49 publications

References 27 publications

Functional and histological effects of intravaginal electrical stimulation on the pelvic muscles: a study in the rat

Functional and histological effects of intravaginal electrical stimulation on the pelvic muscles: a study in the rat

Effect of Intermittent Low-Frequency Electrical Stimulation on the Rat Gastrocnemius Muscle

Electrostimulation in sports and rehabilitation

Contact Info

Product

Resources

About