Effects of low salinities on growth, fatty acid composition, and transcriptome in Florida pompano (Trachinotus carolinus) at early developmental stages

Bradshaw, David J.; Uribe, Victoria; King, Laura E.; Perricone, Carlie S.; Illan, Gonzalo; Allmon, Elizabeth; Sepúlveda, Marisol; Riche, Marty; Wills, Paul S.; Mejri, Sahar

doi:10.1016/j.aquaculture.2022.738964

Cited by 5 publications

(6 citation statements)

References 53 publications

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“…An upregulation in immune-related genes due to osmotic stress was revealed in both cobia, (Rachycentron canadum) (Cao et al, 2020), and silvery pomfret (Pampus argenteus) . In contrast, we have observed a downregulation of immune response gene sets in the 10 ppt larvae (Bradshaw et al, 2023). This downregulation in immune response genes may indicate that Florida pompano has an isosmotic point close to 10 ppt.…”

Section: Whole Transcriptomicscontrasting

confidence: 74%

“…Their expression levels were not significantly different among salinities. Although there was a higher percentage of gramnegative PPG along with more apparent epithelial lifting at lower salinities, the downregulation of immune response related genes sets (Bradshaw et al, 2023) and limited upregulation of NOD1-related genes at lower salinities suggests that either these bacteria were nonvirulent, or the rearing conditions were not optimal for infection. On the other hand, RNA-Seq as performed on whole larvae, thus if there was stress in a particular body part, like the gills, its responses may be masked by the rest of the whole body gene expression generally not being in a stressed state.…”

Section: Whole Transcriptomicsmentioning

confidence: 97%

“…RNA extraction and transcriptomics analysis occurred as previously reported in another article published on this study (Bradshaw et al, 2023). Briefly, total RNA was extracted from up to 0.25 g whole homogenized larvae with the RNeasy Mini Kit (Qiagen, Hilden, Germany).…”

Section: Whole Transcriptomicsmentioning

confidence: 99%

“…Log-fold changes were separately imported into RStudio and used in Gene Set Enrichment Analysis. General whole transcriptomics results and interpretation of the DEGs, Gene Set Enrichment Analysis, and fatty acid biosynthesis genes can be found in our previous article (Bradshaw et al, 2023). In this article we further analyzed the trends for 12 target genes that were chosen due to past studies demonstrating that their expression was significantly affected by salinity changes or pathogenic infection (Zhou et al, 2015;Maekawa et al, 2017;Si et al, 2018;Gong et al, 2020;Lin et al, 2020;Peng et al, 2022).…”

Section: Whole Transcriptomicsmentioning

confidence: 99%

“…near iso-osmotic, salinity on larval rearing. This research work contains data that was collected during the same study as data reported in a previous manuscript, that assessed the effects of salinity on biometrics, fatty acids, and genes linked to osmoregulation and fatty acids in Florida pompano larviculture (Bradshaw et al, 2023). In this study, we have provided a multidisciplinary view of how Florida pompano larvae respond to low salinities using histological techniques, 16S DNA sequencing, and whole transcriptomics.…”

Section: Introductionmentioning

confidence: 97%

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Commercial production of Florida pompano (Trachinotus carolinus) larvae at low salinity induces variable changes in whole-larvae microbial diversity, gene expression, and gill histopathology

et al. 2023

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IntroductionSalinity presents economic and technical challenges in land-based recirculating aquaculture systems (RAS) in the U.S. warm water marine finfish aquaculture industry. Many studies have shown euryhaline fish reared at salinities closer to their iso-osmotic salinity can yield enhanced production performance as well as potential reduced costs to farms. However, there is potential for osmotic stress in fish larvae to negatively impact larvae microbiome and innate immune system. Florida pompano (Trachinotus carolinus) is a popular sportfish has been targeted for land-based RAS due to its impressive market value and euryhaline capacity. This study investigated the impacts of rearing Florida pompano larvae at salinities closer to their iso-osmotic salinity.Materials and methodsLarvae were cultured at 10, 20, and 30 ppt in triplicates, and larvae samples were collected for histopathology, microbiome, and whole transcriptomics analysis every three days from hatching until the time of weaning (24 days post hatch [DPH]). Water samples were also taken for microbiome analysis on every other larval sampling day. DiscussionThese changes were driven more by metamorphosis, causing an increase in expression of antioxidant genes (cat, gss, gsto1, and scara3) than by the presence of potentially pathogenic genera, which failed to induce an immune response (low or unchanged expression of downstream elements of the NOD1 or TLR5 pathways). These findings provide baseline information on Florida pompano low salinity tolerance in larviculture during early developmental stages. In addition, we have shown minimal effects on the immune system at salinities as low as 10 ppt. This work has important implications for larval health management and can be used to refine and direct future research regarding improving commercial production of warm water marine species

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Section: Whole Transcriptomicscontrasting

confidence: 74%

Section: Whole Transcriptomicsmentioning

confidence: 97%

Section: Whole Transcriptomicsmentioning

confidence: 99%