Effects of lysophosphatidic acid on human periodontal ligament stem cells from teeth extracted from dental patients

Abstract

Despite their potential applications in future regenerative medicine, periodontal ligament stem cells (PDLSCs) are difficult to obtain in large amounts from patients. Therefore, maintaining stemness while expanding the cell numbers for medical use is the key to transitioning PDLSCs from the bench to the clinic. Lysophosphatidic acid (LPA), which is present in the human body and saliva, is a signaling molecule derived from phospholipids. In this study, we examined the effects of LPA on stemness maintenance in human PDLSCs. Several spindle-shaped and fibroblast-like periodontal ligament stem-like cell lines were established from PDLSC isolation. Among these cell lines, the most morphologically appropriate cell line was characterized. The expression levels of OCT4 , NANOG (a stem cell marker), and CD90 (a mesenchymal stem cell marker) were high. However, CD73 (a negative marker of mesenchymal stem cells) expression was not observed. Notably, immunofluorescence analysis identified the expression of STRO-1 , CD14 6 (a mesenchymal stem cell marker), and sex determining region Y-box 2 at the protein level. In addition, lipid droplets were stained by Oil red O after the induction of adipogenesis for 21 days, and mineralized nodules were stained by Alizarin Red S after the induction of osteogenesis for 14 days. Alkaline phosphate staining also demonstrated the occurrence of osteogenesis. In summary, we established a human PDLSC line, which could be applied as a cell source for tissue regeneration in dental patients. However, further studies are needed to determine the detailed effects of LPA on PDLSCs.

Lysophosphatidic Acid/Polydopamine-Modified nHA Composite Scaffolds for Enhanced Osteogenesis via Upregulating the Wnt/Beta-Catenin Pathway

“…Lysophosphatidic acid (LPA) is a serum-derived growth factor that occurs naturally in humans and has a growth factor-like effect in various cells such as osteoblasts, osteoclasts, and adipocytes. , LPA-specific G protein-coupled receptors, LPA 1 –LPA 6 , have been identified and expressed in different cell types, including skeletal myoblasts, osteocytes, human-induced pluripotent stem cells, and epithelial cells, which is involved in a wide range of biological processes including cell adhesion, proliferation, differentiation, migration, apoptosis, chemotaxis, and survival. − LPA1/3 receptors have been shown to promote osteoblast proliferation, chemotaxis, and differentiation. , Moreover, our previous research also confirmed that the GBR scaffolds prepared by loading LPA nanoparticles into the scaffold by coaxial electrospinning technology could enhance the osteogenic performance of cells . These convincing reports highlight the potential application of LPA as an effective active material for tissue regeneration.…”

“…20,21 Lysophosphatidic acid (LPA) is a serum-derived growth factor that occurs naturally in humans and has a growth factorlike effect in various cells such as osteoblasts, osteoclasts, and adipocytes. 22,23 LPA-specific G protein-coupled receptors, LPA 1 −LPA 6 , have been identified and expressed in different cell types, including skeletal myoblasts, osteocytes, humaninduced pluripotent stem cells, and epithelial cells, which is involved in a wide range of biological processes including cell adhesion, proliferation, differentiation, migration, apoptosis, chemotaxis, and survival. 24−27 LPA1/3 receptors have been shown to promote osteoblast proliferation, chemotaxis, and differentiation.…”

Triclosan inhibits the activation of human periodontal ligament fibroblasts induced by lipopolysaccharide from <i>Porphyromonas gingivalis</i>

“…Human periodontal ligament fibroblasts (HPDLFs), similar to gingival fibroblasts, synthesize the collagen present in and supporting the periodontal tissues [ 9 – 10 ] . When activated by components of exogenous pathogens, e.g.…”