Effects of melatonin and dexpanthenol on antioxidant parameters when combined with estrogen treatment in ovariectomized rats

Abstract

The purpose of the study was to assess whether it is possible to reduce the oxidative damage using antioxidant agents combined with hormone replacement therapy after menopause. In this prospective experimental study, 50 mature female Wistar albino rats weighing 270-310 g were used. Rats were divided into the following six groups: (1) Ovx group (n=7): the animals underwent bilateral ovariectomy. No drug was administered following bilateral ovariectomy. (2) Ovx+ E 2 group (n=7): bilateral ovariectomy+17β-estradiol (100 μg/kg/day); (3) Ovx+E 2 +MT5 group (n=7): bilateral ovariectomy+17β-estradiol (100 μg/kg/day)+mel-atonin (5 mg/kg/day); (4) Ovx+E 2 +MT20 group (n=7): bilateral ovariectomy+17β-estradiol (100 μg/kg/day)+ melatonin (20 mg/kg/day); (5) Ovx+E 2 +Dxp250 group (n = 7): bilateral ovariectomy + 17β-estradiol (100 μg/kg/day)+dexpanthenol (250 mg/kg/day); (6) Ovx+E 2 +Dxp500 group (n=7): bilateral ovariectomy + 17β-estradiol (100 μg/kg/day) + dexpanthenol (500 mg/kg/day), and the activity of these antioxidative enzymes and oxidative stress products were measured. Enzymatic activity levels of catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase(GSH-Px), and glutathione reductase and levels of free radicals (malondialdehyde (MDA) and nitric oxide) were both analyzed. We observed an increase in the level of GSH activity, but no significant differences in levels of CAT, SOD, and GSH-Px enzymatic activity and in levels of free radical MDA following 17β-estradiol or additional antioxidant treatment (melatonin or dexpanthenol). Despite the present study indicating that the addition of melatonin and dexpanthenol into the hormone replacement therapy regimen may contribute to the antioxidant effect of estrogen, the existence of limited data in this field indicates that further studies are warranted.

Effects of menopause and diabetes on the rat parotid glands: A histopathological and stereological study

“…Oxidative stress which occurs as a result of declining estrogen levels and response to that stress has been researched in many studies. [3][4][5][6] Oxidative stress triggers production of radioactive oxygen spices (ROS) that causes cell or tissue damaging. Increased ROS level that has been displayed for higher risk for some diseases like osteoporosis, cardiovascular disease, or comforts like salivary glands in the body.…”

Effects of Diabetes on Post-Menopausal Rat Submandibular Glands: A Histopathological and Stereological Examination

“…Many studies have investigated the oxidative stress and response to oxidative stress that occur during menopause as a result of declining oestrogen levels [12,13]. Various causes induce oxidative stress, which triggers reactive oxygen spices (ROS) production in tissues.…”

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“…21 Psikolojik harbin ortaya çıkardığı tesir çok hızla yayılabilir ve fertten topluluğa sirayet edebilir. 22 Bu nedenle psikolojik harbin kullandığı yöntem çok önemlidir.…”