Effects of Diabetes on Post-Menopausal Rat Submandibular Glands: A Histopathological and Stereological Examination

Bűyűk, Başak; Parlak, Seçil Nazife; Keleş, Osman Nuri; Can, İsmail Özgür; Yetim, Zeliha; Toktay, Erdem; Selli, Jale; Ünal, Bünyamin

doi:10.5152/eurasianjmed.2015.80

Cited by 10 publications

(6 citation statements)

References 48 publications

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“…In this research, secretory granules were accumulated in the apical cytoplasm of striated duct lining cellsattributed to oxidative stress and altered cytology of acinar and granular ductal cells which lead to changes in the secretory proteins and disturbed their secretory function [38] . Noteworthy, the striated ducts endocytose proteins are secreted by the acinar cells in a very low rate in normal animals, but under certain pathological conditions extensive endocytosis takes place to get rid of the abnormal proteins present in saliva before it reaches the mouth, which is considered as a defense mechanism [50] .…”

Section: Discussionmentioning

confidence: 82%

“…As a result, this secretory material accumulates in the cytoplasm of the acinar cells leading to cellular degeneration, atrophy of the cytoplasm and nuclei, and even cell death. This explains the induced structural changes such as widely separated shrunken acini, distortion and loss of some intralobular acini and ducts [38][39][40] .…”

Section: Discussionmentioning

confidence: 99%

“…The increase in the size and number of the myoepithelial cells occurs in the degenerated acini and ducts areas to compensate this degeneration. The myoepithelial cells in the degenerated areas have a function which may be phagocytic or stem cells [38] . Moreover, in the gland parenchymal injury, the myoepithelial cells capacity were activated aiming to preserve the secretory function of the cells.…”

Section: Discussionmentioning

confidence: 99%

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Effect of a caffeinated energy drink and its withdrawal on the submandibular salivary gland of adult male albino rats: A histological and immunohistochemical study

Kassab

Tawfik

2018

Egyptian Journal of Histology

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Introduction: Energy drinks provide the consumer witha combination of stimulants and energy boosters that increases the physical endurance, concentration, muscular performance and mood. Despite their benefits, many health hazards occur that may be attributed to its caffeine content. Aim: To study the effect of a caffeinated energy drink on the histological structure of submandibular salivary gland of albino rats and evaluation of the effect of its withdrawal. Materials and Methods: Thirty adult male albino rats were divided into 3 equal groups: control group, group II (received the energy drink in a dose 3.75 ml/Kg daily orally for 8 weeks), group III (received the energy drink in a dose 3.75 ml/Kg daily orally for 8 weeks, and left for another 8 weeks without treatment). The submandibular salivary gland specimens were taken and prepared for light and electron microscopic study and immunohistochemical staining for myoepithelial cells using anti α-SMA antibody. Results: Specimens from group II showed disturbance of the normal structure of the submandibular salivary glands. The lining epithelium of the acini and ducts showed cytoplasmic vacuoles with compressed or pyknotic nuclei. Disturbance of the basal striation of the cells lining striated ducts. There was deposition of abundant collagen fibers between the lobules and around the acini and ducts. A statistically highly significant increase in α-SMA immunoreaction was noticed around the acini, intercalated, striated and granular ducts. Ultrastructurally, fusion of the secretory granules, swollen mitochondria, dilatation and fragmentation of rough endoplasmic reticulum of the acinar cells were observed. Amelioration of the previous histological changes was detected in group III after withdrawal of the caffeinated energy drink. Conclusion: The caffeinated energy drink induced structural changes in the submandibular salivary gland of Albino rats which were partially ameliorated by its withdrawal. So, excessive intake of the caffeinated energy drinks should be considered as health hazard for human.

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Section: Discussionmentioning

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Effect of a caffeinated energy drink and its withdrawal on the submandibular salivary gland of adult male albino rats: A histological and immunohistochemical study

Kassab

Tawfik

2018

Egyptian Journal of Histology

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“…Induction of diabetes with alloxan can cause certain functional and morphological changes in the submandibular glands affecting their secretions. This could be attributed to an oxidative stress causing degeneration and apoptosis in submandibular stromal cells [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Postnatal changes in the development of rat submandibular glands in offspring of diabetic mothers: Biochemical, histological and ultrastructural study

2018

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Development and maturation of submandibular salivary glands are influenced by intrauterine diabetic environment. Several studies investigated the effects of diabetes on the salivary glands. However, the effects of maternal diabetes on the submandibular glands of the offspring was not properly examined. Therefore, the present study was designed to describe the changes in the development of the submandibular glands of the offspring of diabetic mothers. The submandibular glands of the offspring of Streptozotocin (STZ)-induced diabetic female rats were examined at two and four weeks after birth. Detection of mRNA demonstrated that maternal diabetes affects the level of different indicators. The reduction of expression of epidermal growth factor (EGF); a protein mitogen, cytokeratin 5 (CK5); an epithelial cell progenitor, CK7 and aquaporin 5 (AQP5); differentiation markers and B cell lymphoma 2 (Bcl2); an antiapoptotic marker were found. Increase in Bcl2-associated X protein (Bax); an apoptotic marker was detected. These changes indicate their effects on saliva secretion, glands tumorigenesis, growth of normal oral flora and oral microbes, with decreased protein synthesis and production of xerostomia and dental caries. Loss of normal glandular architecture, significant increase in fibrosis, by the detection of collagen fibers, and stagnation of secretory granules were found with atrophic changes in the acinar cells. Marked defect of polysaccharides in the acinar cells, denoting functional changes, was manifested by significant reduction of the intensity of periodic acid-Schiff (PAS) reaction. The positive immunoreactivity of caspase-3, denoting cellular apoptosis, and minimal reaction of alpha-smooth muscle actin (α SMA) and proliferating cell nuclear antigen (PCNA) were evident in the offspring of diabetic mothers. We conclude that maternal diabetes produces degenerative effects in the structure and function of the submandibular salivary glands of the offspring, reflecting possible influences on their secretory activity affecting oral and digestive health.

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“…Así tenemos, a Noorafshan, quien observó una disminución del volumen total de los conductos contorneados granulares y de los acinos serosos en ratas, a las cuales se les indujo hipotiroidismo mediante la administración de metimazol. Este mismo autor, en 2006, produjo diabetes en ratas por medio de estreptozotocina, señalando la reducción del volumen total de los acinos serosos Buyuk et al (2015),. determinaron un descenso del área de los acinos seromucosos y de los conductos contorneados granulares, en ratas postmenopáusicas (por extirpación ovárica) y diabéticas (por administración de aloxano) Ali (2018),.…”

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Características Morfocuantitativas de la Glándula Submandibular de Ratón (Mus musculus)

et al. 2020

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OCAMPO, J.; VÁSQUEZ, B.; SANDOVAL, C.; NAVARRETE, J.; HAIDAR, Z. S. & OLATE, S. Características morfocuantitativas de la glándula submandibular de ratón (Mus musculus). Int. J. Morphol., 38(3):570-577, 2020. RESUMEN:El ratón (Mus musculus) es un buen modelo animal de experimentación, donde sus glándulas salivales han sido utilizadas en estudios, especialmente de tipo preclínico. Sin embargo, las diferencias en la morfología de estas glándulas y, en particular de las submandibulares, entre humanos y roedores no son de conocimiento común. Por lo anterior, el objetivo de este estudio fue conocer las características morfocuantitativos de la glándula submandibular del ratón macho C57BL/6. Se utilizaron cinco ratones macho, cepa C57BL/ 6, de 11 a 13 semanas de nacidos, con un peso promedio de 30 g. Luego de su ambientación, los ratones fueron eutanasiados. Para el análisis histológico, las glándulas fueron extraídas y fijadas en formalina tamponada por 48 horas, procesada para su inclusión en paraplast y teñidas con hematoxilina y eosina, azul alcián pH 2,5, ácido peryódico de Schiff y Picrosirius Red. Se determinaron las características estereológicas de densidad de longitud (L Vcond ), de volumen (V Vcond ) y de superficie (S Vcond ) de los conductos. En las células acinares se evaluó la densidad de número (N Vcel ) y la densidad de volumen (V Vcel ). Para cada variable se obtuvo la media ± desviación estándar. La glándula submandibular se encontraba organizada de manera lobulada, cuyo parénquima era de tipo túbuloacinar mixto con predominio seroso, que se conectaba a través de conductos. Las células acinares presentaban reacción positiva para azul alcián y ácido peryódico de Schiff. El estroma de la glándula contenía principalmente colágeno tipo 1. Los resultados de las mediciones estereológicas fueron: L Vcond = 220,02 ± 50,51 mm/mm 3 ; V Vcond = 50,48 ± 9,55 %; S Vcond = 26,39 ± 5,45 mm 2 /mm 3 ; N Vcel = 16,85 x 104 ± 9,13 × 10 4 /mm 3 ; V Vcel = 24,98 ± 6,22 %. Con los análisis presentados es posible realizar investigaciones experimentales, considerando las similitudes y diferencias morfocuantitativas que presenta la glándula submandibular de ratón macho, cepa C57BL/6 con la del humano.

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Effects of Diabetes on Post-Menopausal Rat Submandibular Glands: A Histopathological and Stereological Examination

Cited by 10 publications

References 48 publications

Effect of a caffeinated energy drink and its withdrawal on the submandibular salivary gland of adult male albino rats: A histological and immunohistochemical study

Effect of a caffeinated energy drink and its withdrawal on the submandibular salivary gland of adult male albino rats: A histological and immunohistochemical study

Postnatal changes in the development of rat submandibular glands in offspring of diabetic mothers: Biochemical, histological and ultrastructural study

Características Morfocuantitativas de la Glándula Submandibular de Ratón (Mus musculus)

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