Effects of methyl jasmonate and salicylic acid on the production of bilobalide and ginkgolides in cell cultures of Ginkgo biloba

Kang, Shin-Min; Jiang, Min; Kim, Yong-Duck; Kang, Young Min; Park, Dong-Jin; Jung, Hwa Jin; Kim, Seon-Won; Choi, Myung-Suk

doi:10.1079/ivp2005719

Cited by 68 publications

(40 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Among them, 0.1 mM resulted in the highest content of bilobalide and ginkgolides A, B, and C. In the present study, we were able to produce 1.68 times increased production of bilobalide and ginkgolides A, B, and C. Therefore, to achieve maximum stimulation of the synthesis of bilobalide and ginkgolides A, B, and C, both the concentrations and the incubation times of the elicitors need to be optimized. This conclusion agrees with those reached for several other metabolite and plant cell systems: the levels of ginkgolides A and B after treatment with 1.0 mM MJ increased 4.3 and 8.2 times over control after 12 h (Kang et al 2006) and isoflavone production was enhanced by adding 100 μM of MJ for about 72 h in hairy root cultures of soybean (Theboral et al 2014). With SA elicitation, 0.1 mM increased the production of bilobalide and ginkgolides A, B and C by 1.8, 1.7, 1.8 and 2.2 times, respectively, higher than the quantity produced using unelicited cultures (Fig.…”

Section: The Effect Of Mj Sa and Mj + Sa In Enhancing Bilobalide Ansupporting

confidence: 91%

“…Exogenously applied SA has been shown to affect a variety of processes in plants, including stomatal closure, seed germination, fruit yield, and glycolysis (Klessig and Malamy 1994). Furthermore, Kang et al (2006) reported that treatment with MJ and SA decreased cell growth in a cell suspension culture of G. biloba.…”

Section: Accumulation Of Bilobalide and Ginkgolides In G Biloba Cellmentioning

confidence: 99%

See 1 more Smart Citation

Effect of methyl jasmonate and salycilic acid synergism on enhancement of bilobalide and ginkgolide production by immobilized cell cultures of Ginkgo biloba

Sukito¹,

Tachibana

2016

Bioresour. Bioprocess.

View full text Add to dashboard Cite

Background: Bilobalide and ginkgolides are reported to be present only in Ginkgo biloba. However, only trace amounts of bilobalide and ginkgolides are contained in the ginkgo leaves. Nowadays, there has been considerable interest in plant cell cultures as a potential alternative to traditional agriculture for the industrial production of secondary metabolites. Much effort has been put into the use of in vitro cultures as one attractive biotechnological strategy for producing bilobalide and ginkgolides of commercial interest. The aim of this study was to enhance the production of bilobalide and ginkgolides A, B, and C in cell cultures of G. biloba using immobilized cell cultures and the process of elicitation.Results: Based on the effect of the immobilization components on the free cell suspension cultures, it was considered that the jute fiber acted as an elicitor and forced the cells to release the product into the culture media. The resulting biomass was approximately 1.4 times higher than in the cell suspension cultures, and the production of bilobalide and ginkgolides A, B, and C increased 5.0, 3.3, 6.1, and 4.1 times, respectively. Eliciting with methyl jasmonate (MJ) and salicylic acid (SA) in the immobilized cells enhanced bilobalide and ginkgolides A, B, and C, compared with the unelicited controls. The highest accumulation was observed using a combination of 0.1 mM MJ + 0.1 mM SA in the immobilized cells, which produced 1.78, 1.95, 2.05, and 2.95 times more bilobalide and ginkgolides A, B, and C, respectively, than the controls. Conclusions:The positive effects of immobilized cell cultures using jute fiber and the synergism effect of SA and MJ on immobilized cells of G. biloba appear to be the optimal conditions for continuous in vitro production for commercial purposes. This is the first report on analyzing the effects of jute fiber as immobilized cell material on G. biloba cell cultures and the synergism of MJ and SA on bilobalide and ginkgolide production.

show abstract

Section: The Effect Of Mj Sa and Mj + Sa In Enhancing Bilobalide Ansupporting

confidence: 91%

Section: Accumulation Of Bilobalide and Ginkgolides In G Biloba Cellmentioning

confidence: 99%

Effect of methyl jasmonate and salycilic acid synergism on enhancement of bilobalide and ginkgolide production by immobilized cell cultures of Ginkgo biloba

Sukito¹,

Tachibana

2016

Bioresour. Bioprocess.

View full text Add to dashboard Cite

show abstract

“…Second, the collar originated-callus had also the highest propagation rate. Similar observations have been reported by others (24)(25). In view of the results of their research on taxol production in the Taxus suspension cultures, Kim et al (2004) concluded that cells with high division rate and biomass development at the proliferation stage under the effect of elicitors could produce higher secondary metabolites at the production stage (24).…”

Section: Discussionsupporting

confidence: 80%

“…SA activates signaling pathways that cause secondary metabolite production in plant cultures (28). For instance, application of SA (1 mM) in the culture of Ginkgo biloba resulted in an increase of 3.1 and 6.1 times in the gingkolide A and ginkgolide B production, Iran respectively (25). The results illustrated in Figure 5 indicate that SA was able to influence the production capacity of all kinds of A. euchroma calli.…”

Section: Discussionmentioning

confidence: 96%

See 1 more Smart Citation

Enhancement of Shikalkin Production in Arnebia euchroma Callus by a Fungal Elicitor, Rhizoctonia solani

2015

View full text Add to dashboard Cite

Background: There is a growing demand for mass production of shikalkin (a natural pigment consisted of shikonin and alkannin) due to its increasing applications in cosmetics, pharmaceutical and nutrition industries. The root of Iranian Arnebia euchroma produces shikalkin. The promising capability of this plant for shikalkin production has already been demonstrated in cell culture studies. Objectives: Elicitation effect of Rhizoctonia solani (R. solani) in comparison with the effects of Cu 2+ , methyl jasmonate (MJ), and salicylic acid (SA) on the shikalkin production was investigated in A. euchroma callus. Materials and Methods:The calli from different origins (leaf, collar and root) were proliferated on a modified LinsmaierSkoog (mLS) medium and were subsequently transferred onto the pigment production medium containing various amounts of the desirable elicitor. Observations were quantified and the pigment production was precisely measured spectrophotometrically. Results: Pigment biosynthesis was induced on White medium containing IAA (1 μM) and kinetin (10 μM) in dark at 25°C. Use of R. solani increased the pigment production by 7 fold greater than normal White medium. Cu 2+ only doubled the shikalkin production. MJ and SA showed enhancing effects comparable to that of Cu 2+ . Discussions: It is assumed that upon binding of the polysaccharides of the fungal cells to the plant cell surface, a cascade of signaling is initiated that led to expression of genes involving in the biosynthesis of shikalkin.

show abstract

An Update on Core Jasmonate Signalling Networks, Physiological Scenarios, and Health Applications

Pérez‐Salamó

Krasauskas

Gates

et al. 2019

Annual Plant Reviews Online

View full text Add to dashboard Cite

Jasmonic acid, its precursors in the biosynthetic pathway and derivatives are lipid‐derived signalling molecules. Together with other plant hormones, they regulate responses to stress (biotic and abiotic), mediate defence (against pathogens and herbivores), and a plethora of processes including growth and reproductive development in different plant species. The discovery of jasmonates is more recent than that of auxin, abscisic acid, cytokinins, gibberellic acid, ethylene, and salicylic acid; however, their role in regulating plant developmental plasticity is second to none. Since the 1990s, research made substantial advances and added to the crosstalk with the abovementioned other hormones and signalling pathways. Building on the knowledge of over two decades, an update on the research in JAs signalling components regulating plant responses to developmental and environmental cues is provided here. Information in Arabidopsis thaliana is integrated with reports on other plants. Recent findings in the regulation of plant responses to biotics and abiotic stressors and evidence implicating JAs in the regulation of the trade‐off between growth and stress will be reported. Health applications for JAs and JAs‐induced secondary metabolism, bridging the gap between biotechnology and traditional medicine, will illustrate the practical and societal relevance of investigating the functions of such compounds.

show abstract

Effects of methyl jasmonate and salicylic acid on the production of bilobalide and ginkgolides in cell cultures of Ginkgo biloba

Cited by 68 publications

References 21 publications

Effect of methyl jasmonate and salycilic acid synergism on enhancement of bilobalide and ginkgolide production by immobilized cell cultures of Ginkgo biloba

Effect of methyl jasmonate and salycilic acid synergism on enhancement of bilobalide and ginkgolide production by immobilized cell cultures of Ginkgo biloba

Enhancement of Shikalkin Production in Arnebia euchroma Callus by a Fungal Elicitor, Rhizoctonia solani

An Update on Core Jasmonate Signalling Networks, Physiological Scenarios, and Health Applications

Contact Info

Product

Resources

About