Effects of methyl jasmonate and abscisic acid on anthocyanin biosynthesis in callus cultures of red-fleshed apple (Malus sieversii f. niedzwetzkyana)

Sun, Jingjing; Wang, Yicheng; Chen, Xuesen; Gong, Xiaojun; Wang, Nan; Ma, Li; Qiu, Yanfen; Wang, Yanling; Feng, Shouqian

doi:10.1007/s11240-017-1217-4

Cited by 35 publications

(21 citation statements)

References 70 publications

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“…The correlation analysis showed that there was a positive correlation between the anthocyanin content in the peel and the exogenous ABA content, but the difference was not significant ( Table 3). Unlike the results of our study, Sun et al (2017) found that exogenous ABA inhibited anthocyanin synthesis in red-fleshed apple callus. Through gene expression analysis, they found that ABA inhibited the expression of anthocyanin structural genes, thereby inhibiting anthocyanin synthesis.…”

Section: Effects Of Exogenous Aba On the Synthesis Of Endogenous Aba contrasting

confidence: 99%

Exogenous Abscisic Acid Regulates Distribution of 13C and 15N and Anthocyanin Synthesis in ‘Red Fuji’ Apple Fruit Under High Nitrogen Supply

et al. 2020

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In order to improve the problem of poor coloring caused by high fruit nitrogen in apple production, we studied the effects of different concentrations of abscisic acid (ABA: 0, 50, 100, and 150 mg/L) and fluridone (ABA biosynthesis inhibitor) on the fruit of 'Red Fuji' apple (Malus Domestica Borkh.) in the late stage of apple development (135 days after blooming) in 2017 and 2018. The effects of these treatments on the distribution of 13 C and 15 N and anthocyanin synthesis in fruit were studied. The results showed that the expression levels of ABA synthesis and receptor genes in the peel and flesh were upregulated by exogenous ABA treatment. An appropriate concentration of ABA significantly increased the expression of anthocyanin synthesis genes and transcription factors and increased the content of anthocyanin in the peel. The results of 13 C and 15 N double isotope labeling showed that exogenous ABA coordinated the carbon-nitrogen nutrient of apple fruit in the late stage of the development, reduced the accumulation of fruit nitrogen, increased the accumulation of fruit carbon and sugar, provided a substrate for anthocyanin synthesis, or promoted anthocyanin synthesis through the sugar signal regulation mechanism. Comprehensive analysis showed that the application of 100 mg/L ABA effectively improved the problem of poor coloring caused by high fruit nitrogen in the late stage of apple development and is beneficial to the accumulation of carbon in fruit and the formation of color.

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Section: Effects Of Exogenous Aba On the Synthesis Of Endogenous Aba contrasting

confidence: 99%

Exogenous Abscisic Acid Regulates Distribution of 13C and 15N and Anthocyanin Synthesis in ‘Red Fuji’ Apple Fruit Under High Nitrogen Supply

et al. 2020

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“…In contrast, Ben-Arie et al (1971) and Stern et al (2010) found that auxin stimulated anthocyanin production in the apple fruit skin. Subsequently, Sun et al (2017) demonstrated that methyl JA treatment enhanced anthocyanin synthesis in calli, whereas anthocyanin accumulation was inhibited by adding abscisic acid. The inductive effect of methyl JA in calli is consistent with that observed in previous studies on fruit skin (Rudell et al, 2005), whereas the effects of abscisic acid treatment on calli and fruit skin may differ (Iamsub et al, 2009).…”

Section: ) Other Factorsmentioning

confidence: 99%

Anthocyanin Biosynthesis in Apple Fruit

Honda

Moriya

2018

The Hortic J

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Anthocyanin accumulation is responsible for the red color of the skin and flesh of apple fruits (Malus × domestica Borkh.), and redder fruits are more marketable. Pigmentation in the skin of apple fruit varies among different cultivars and is influenced by environmental factors, including temperature conditions and the level of sunlight irradiation. Because warmer temperatures suppress anthocyanin synthesis in the skin of apple fruit, there are increasing concerns that global warming may be detrimental to fruit pigmentation. Recent molecular studies have revealed that the MdMYB1 gene, which encodes a transcription factor, plays a critical role in regulating anthocyanin synthesis in both the skin and flesh of apple fruits. A marker-assisted selection process has been developed to identify MdMYB1 genotypes and predict those fruits that will develop redder skin. These apples may be better adapted to a warmer global climate. The application of hormones can also increase the level of pigmentation in fruit skin, and plant growth regulators such as ethylene and jasmonate are commercially available. The mechanisms that regulate anthocyanin biosynthesis in the flesh of red-fleshed apple fruit appear to partially differ from those that function in the skin of red-skinned fruit. In the flesh of red-fleshed fruit, the pigment accumulates under dark conditions, whereas no anthocyanin is synthesized in the skin of bagged apple fruit. Conversely, in both red-skinned and red-fleshed apple fruits, warmer temperatures inhibit anthocyanin accumulation. Further studies on the regulation of anthocyanin synthesis in the flesh of red-fleshed apple fruit are necessary.

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“…Calli induced as previously described were cultured on Murashige and Skoog (MS) medium supplemented with 2 μmol/L 6-benzylaminopurine and 5, 10, 20, or 40 μmol/L NAA 43 . The pH of all media was adjusted to 5.8 ± 0.1.…”

Section: Methodsmentioning

confidence: 99%

Auxin regulates anthocyanin biosynthesis through the Aux/IAA–ARF signaling pathway in apple

Wang

et al. 2018

Hortic Res

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139

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Auxin signaling, which is crucial for normal plant growth and development, mainly depends on ARF–Aux/IAA interactions. However, little is known regarding the regulatory effects of auxin signaling on anthocyanin metabolism in apple (Malus domestica). We investigated the functions of MdARF13, which contains a repression domain and is localized to the nucleus. This protein was observed to interact with the Aux/IAA repressor, MdIAA121, through its C-terminal dimerization domain. Protein degradation experiments proved that MdIAA121 is an unstable protein that is degraded by the 26S proteasome. Additionally, MdIAA121 stability is affected by the application of exogenous auxin. Furthermore, the overexpression of MdIAA121 and MdARF13 in transgenic red-fleshed apple calli weakened the inhibitory effect of MdARF13 on anthocyanin biosynthesis. These results indicate that the degradation of MdIAA121 induced by auxin treatment can release MdARF13, which acts as a negative regulator of the anthocyanin metabolic pathway. Additionally, yeast two-hybrid, bimolecular fluorescence complementation, and pull-down assays confirmed that MdMYB10 interacts with MdARF13. A subsequent electrophoretic mobility shift assay and yeast one-hybrid assay demonstrated that MdARF13 directly binds to the promoter of MdDFR, which is an anthocyanin pathway structural gene. Interestingly, chromatin immunoprecipitation–quantitative real-time PCR results indicated that the overexpression of MdIAA121 clearly inhibits the recruitment of MdARF13 to the MdDFR promoter. Our findings further characterized the mechanism underlying the regulation of anthocyanin biosynthesis via Aux/IAA–ARF signaling.

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Effects of methyl jasmonate and abscisic acid on anthocyanin biosynthesis in callus cultures of red-fleshed apple (Malus sieversii f. niedzwetzkyana)

Cited by 35 publications

References 70 publications

Exogenous Abscisic Acid Regulates Distribution of 13C and 15N and Anthocyanin Synthesis in ‘Red Fuji’ Apple Fruit Under High Nitrogen Supply

Exogenous Abscisic Acid Regulates Distribution of 13C and 15N and Anthocyanin Synthesis in ‘Red Fuji’ Apple Fruit Under High Nitrogen Supply

Anthocyanin Biosynthesis in Apple Fruit

Auxin regulates anthocyanin biosynthesis through the Aux/IAA–ARF signaling pathway in apple

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