Effects of Multimodal Analgesic Protocol, with Buprenorphine and Meloxicam, on Mice Well-Being: A Dose Finding Study

Furumoto, Kayo; Ogita, Kumi; Kamisaka, Tomomi; Kawasumi, Asami; Takata, Koushi; Maeta, Noritaka; Itoi, Takamasa; Nohara, Masakatsu; Saeki, Kiyomi; Kanda, Teppei

doi:10.3390/ani11123420

Cited by 3 publications

(1 citation statement)

References 21 publications

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“…After the operation, we put them on the heating pad to keep them warm. When the righting reflex was confirmed, 0.1 mg/kg buprenorphine (Y0001108, MREDA, Beijing, China) was injected subcutaneously with 28.5-gauge insulin needles [ 25 ]. Subsequently, they were returned to their cages.…”

Section: Methodsmentioning

confidence: 99%

The Effect of Different Preoperative Depilation Ways on the Healing of Wounded Skin in Mice

Jia

Zhang

2022

Animals

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Hair removal is necessary before operating on animals with dense hair layers. To provide an appropriate hair removal method and maintain animal welfare, we introduced four commonly used depilation methods—namely, scissors shearing, electric shaving, depilatory cream, and sodium sulfide, and made systematic comparisons, instead of only examining one or two methods, as reported in the past. To further assess convenience and possible effects on skin wound healing, we performed a skin trauma model after depilation in C57BL/6J mice and recorded wound healing time. Meanwhile, the skin tissues around the wound were stained with H&E and Masson. The results showed that the wound contraction rate of the sodium sulfide group was significantly lower than other groups at different points in time. Furthermore, depilatory cream and sodium sulfide methods could induce a topical inflammatory response on the third day after the operation and delay the regeneration of collagen fibers. We concluded that sodium sulfide depilatory has a significant negative effect on wound healing. Depilatory cream is gentler, with mild skin irritation and symptoms of inflammation. The electric shaving method is more convenient and safer, and thus could be the best choice for preoperative depilation.

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Section: Methodsmentioning

confidence: 99%

The Effect of Different Preoperative Depilation Ways on the Healing of Wounded Skin in Mice

Jia

Zhang

2022

Animals

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Efficacy of Supplemental Diet Gels for Preventing Postoperative Weight Loss in Mice ( Mus musculus)

Gates

Alamaw

Jampachaisri

et al. 2023

j am assoc lab anim sci

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This study investigated whether the use of commercially available diet gels prevented the postoperative weight loss associated with major survival surgery in mice. C57BL/6 mice were divided into 3 groups (n = 9 per group) that received moistened chow pellets alone or with one of 2 commercially available diet gels. Mice began receiving the test diets 3 d before surgery (baseline) and were weighed daily for 7 d after surgery. On day 0, mice underwent ventral midline laparotomy, during which the intestines were manipulated for 2 min and a segment of jejunum was briefly clamped. Compared with the baseline value for the same group, body weights for the mice that received moistened chow only were significantly lower on all postoperative days (days 1 through 7). In contrast, body weights of mice that received both moistened chow and diet gel differed from baseline only on days 2 and 3 for one product and were never different from baseline for the other product. This study indicates that the combination of diet gel and moistened chow prevented or mitigated postoperative weight loss after a laparotomy procedure in mice.

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Effects of a Multimodal Approach Using Buprenorphine with/without Meloxicam on Food Intake, Body Weight, Nest Consolidating Behavior, Burrowing Behavior, and Gastrointestinal Tissues in Postoperative Male Mice

Furumoto

Sasaki

Nohara

et al. 2022

Veterinary Sciences

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Distress affects animal welfare and scientific data validity. There is a lack of reports on the effects of multimodal analgesic approaches in mice. In this study, under the hypothesis that a multimodal analgesic protocol using buprenorphine with meloxicam has analgesic effects, we evaluated the effects of a multimodal analgesic protocol using buprenorphine with meloxicam on the well-being of mice during analgesic administration by changing the dosage of meloxicam. A total of 42 Slc:ICR male mice were categorized into nonsurgical and surgical groups (7 mice per group) and treated with an anesthetic (isoflurane) and analgesics (buprenorphine ± meloxicam). Analgesics were administered for 48 h after treatment. Buprenorphine (subcutaneous; 0.1 mg/kg/8 h) and meloxicam (subcutaneous; 0, 2.5, or 5 mg/kg/24 h) were administered twice. Body weight, food intake, nest consolidation score, and latency to burrow were evaluated. A significant decrease in food intake was observed 24 h after treatment, while a significant increase was observed 48 h post-treatment in all groups. Body weight showed a decreasing trend but was not significantly reduced. Furthermore, stomach, duodenum, and jejunum tissues showed no morphological abnormalities. Significant differences in burrow diving scores and the latency to burrow were observed between some groups, but these were not regarded as a consequence of the surgery and/or the meloxicam dose. When buprenorphine and meloxicam were combined, administering up to 5 mg/kg/day of meloxicam for 48 h to male mice after abdominal surgery had no significant negative effects on any tested parameters. In conclusion, a multimodal analgesic protocol of buprenorphine with meloxicam is among the options for increasing well-being in mice following abdominal surgery.

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Effects of Multimodal Analgesic Protocol, with Buprenorphine and Meloxicam, on Mice Well-Being: A Dose Finding Study

Cited by 3 publications

References 21 publications

The Effect of Different Preoperative Depilation Ways on the Healing of Wounded Skin in Mice

The Effect of Different Preoperative Depilation Ways on the Healing of Wounded Skin in Mice

Efficacy of Supplemental Diet Gels for Preventing Postoperative Weight Loss in Mice ( Mus musculus)

Effects of a Multimodal Approach Using Buprenorphine with/without Meloxicam on Food Intake, Body Weight, Nest Consolidating Behavior, Burrowing Behavior, and Gastrointestinal Tissues in Postoperative Male Mice

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