Effects of Mutations in <i>Aedes aegypti</i> Sterol Carrier Protein-2 on the Biological Function of the Protein

Radek, James T.; Dyer, David H.; Lan, Qing

doi:10.1021/bi902026v

Cited by 18 publications

(22 citation statements)

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“…A. aegypti larval lipids reserve includes cholesterol [31] and deficiency in dietary cholesterol has lethal effects on A. aegypti larvae [30]. We have shown that AeSCP-2 selectively mediates cellular cholesterol uptake [18], [32]. It is possible that developing male larvae require the storage of much higher amount of cholesterol than that of females and AeSCP-2 mediated cholesterol uptake from the dietary sources becomes a much more critical factor for the survival of males.…”

Section: Discussionmentioning

confidence: 95%

In Vivo Functional Genomic Studies of Sterol Carrier Protein-2 Gene in the Yellow Fever Mosquito

et al. 2011

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A simple and efficient DNA delivery method to introduce extrachromosomal DNA into mosquito embryos would significantly aid functional genomic studies. The conventional method for delivery of DNA into insects is to inject the DNA directly into the embryos. Taking advantage of the unique aspects of mosquito reproductive physiology during vitellogenesis and an in vivo transfection reagent that mediates DNA uptake in cells via endocytosis, we have developed a new method to introduce DNA into mosquito embryos vertically via microinjection of DNA vectors in vitellogenic females without directly manipulating the embryos. Our method was able to introduce inducible gene expression vectors transiently into F0 mosquitoes to perform functional studies in vivo without transgenic lines. The high efficiency of expression knockdown was reproducible with more than 70% of the F0 individuals showed sufficient gene expression suppression (<30% of the controls' levels). At the cohort level, AeSCP-2 expression knockdown in early instar larvae resulted in detectable phenotypes of the expression deficiency such as high mortality, lowered fertility, and distorted sex ratio after induction of AeSCP-2 siRNA expression in vivo. The results further confirmed the important role of AeSCP-2 in the development and reproduction of A. aegypti. In this study, we proved that extrachromosaomal transient expression of an inducible gene from a DNA vector vertically delivered via vitellogenic females can be used to manipulate gene expression in F0 generation. This new method will be a simple and efficient tool for in vivo functional genomic studies in mosquitoes.

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Section: Discussionmentioning

confidence: 95%

In Vivo Functional Genomic Studies of Sterol Carrier Protein-2 Gene in the Yellow Fever Mosquito

et al. 2011

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“…SCP2 encodes an intracellular sterol carrier protein that facilitates cholesterol uptake in Ae . aegypti cells [59]. Knockdown or chemical inhibition of SCP2 was recently shown to inhibit DENV replication in Ae .…”

Section: Discussionmentioning

confidence: 99%

Engineered Aedes aegypti JAK/STAT Pathway-Mediated Immunity to Dengue Virus

et al. 2017

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We have developed genetically modified Ae. aegypti mosquitoes that activate the conserved antiviral JAK/STAT pathway in the fat body tissue, by overexpressing either the receptor Dome or the Janus kinase Hop by the blood feeding-induced vitellogenin (Vg) promoter. Transgene expression inhibits infection with several dengue virus (DENV) serotypes in the midgut as well as systemically and in the salivary glands. The impact of the transgenes Dome and Hop on mosquito longevity was minimal, but it resulted in a compromised fecundity when compared to wild-type mosquitoes. Overexpression of Dome and Hop resulted in profound transcriptome regulation in the fat body tissue as well as the midgut tissue, pinpointing several expression signatures that reflect mechanisms of DENV restriction. Our transcriptome studies and reverse genetic analyses suggested that enrichment of DENV restriction factor and depletion of DENV host factor transcripts likely accounts for the DENV inhibition, and they allowed us to identify novel factors that modulate infection. Interestingly, the fat body-specific activation of the JAK/STAT pathway did not result in any enhanced resistance to Zika virus (ZIKV) or chikungunya virus (CHIKV) infection, thereby indicating a possible specialization of the pathway’s antiviral role.

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“…Sterol carrier protein-2 (SCP-2) has been identified in several insect species 9,[30][31][32] and is thought to mediate cholesterol uptake and intracellular transportation. 9,11,12,33) Also, several chemical compounds that strongly inhibit the binding between cholesterol and AeSCP-2 (SCP-2 in Aedes aegypti) have been identified.…”

Section: Discussionmentioning

confidence: 99%

Larvicidal activity of .ALPHA.-mangostin in the Colorado potato beetle, Leptinotarsa decemlineata

Kim

Lan

2011

J. Pestic. Sci.

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a-Mangostin, a major xanthone derivative in mangosteen (Garcinia mangostana), has been proven to have a wide range of biological-activities. In this study, we assessed the larvicidal and antifeeding activities of a-mangostin using larvae and adults of the Colorado potato beetle, Leptinotarsa decemlineata Say. a-Mangostin showed larvicidal activity and growth inhibitory effects under non-choice feeding conditions. The LC 50 values with 7-, 14-and 23-day treatment were 63.66 (13.29-279.79) mM, 8.37 (2.67-20.27) mM and 4.09 (0.89-11.81) mM, respectively. Larval development was significantly delayed 3-8 days with a-mangostin treatment. The antifeeding activity of a-mangostin was also tested by leaf disc non-choice and choice methods against larvae and adults of L. decemlineata. a-Mangostin had stronger antifeeding-activity toward adults than toward larvae, whereas there was low lethality in adults associated with higher antifeeding indexes (AFIs). The larvicidal and antifeeding effects suggest that a-mangostin and related compounds should be explored for their potential as botanical insecticide alternatives for control of the Colorado potato beetle.

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Effects of Mutations in Aedes aegypti Sterol Carrier Protein-2 on the Biological Function of the Protein

Cited by 18 publications

References 50 publications

In Vivo Functional Genomic Studies of Sterol Carrier Protein-2 Gene in the Yellow Fever Mosquito

In Vivo Functional Genomic Studies of Sterol Carrier Protein-2 Gene in the Yellow Fever Mosquito

Engineered Aedes aegypti JAK/STAT Pathway-Mediated Immunity to Dengue Virus

Larvicidal activity of .ALPHA.-mangostin in the Colorado potato beetle, Leptinotarsa decemlineata

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