Effects of non-thermal plasma treatment on the polysaccharide from Dendrobium nobile Lindl. And its immune activities in vitro

Fan, Yujiang; Yu, Qingsong; Wang, Gang; Tan, Jingwen; Li, Sha; Pu, Shangrao; Chen, Wenchuan; Xie, Peng; Zhang, Yixin; Zhang, Jiao; Liao, Yixuan; Luo, Aoxue

doi:10.1016/j.ijbiomac.2019.10.260

Cited by 31 publications

(18 citation statements)

References 36 publications

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“…As far as we know, DNLP exhibited in vitro antioxidant activities (Luo et al, 2009; Zhang et al, 2016). Fan et al (2019) showed that DNLP enhanced phagocytosis ability of RAW264.7 cells and promoted secretion of cytokines including tumor necrosis factor‐alpha, interleukin‐6 and interleukin‐1. In addition, DNLP could stimulate calcium signalling pathway and promote tobacco defense against tobacco mosaic virus (Z. Li et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Dendrobium nobile Lindl polysaccharides improve testicular spermatogenic function in streptozotocin‐induced diabetic rats

Lei

Huo

Xie

et al. 2022

Molecular Reproduction Devel

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Dendrobium nobile Lindl polysaccharides (DNLP) exhibited various biological functions. This study aimed to investigate the protective effects of DNLP on testicular spermatogenic function in streptozotocin (STZ)‐induced diabetic rats in comparison with metformin. The blood glucose level was significantly increased and the homeostatic model assessment for insulin resistance (HOMA‐IR) aggravated markedly in diabetic rats. The weight of testis and epididymis, and the sperm number and motility were decreased in the diabetic rats. The pathologic changes occurred in the spermatogenic tubules along with the decreased number of spermatogenic cells, downregulated proliferating cell nuclear antigen (PCNA) and Sirtuin 1 (SIRT1) expression and increased cell apoptosis in the testes. Compared with the model group, DNLP and metformin treatment significantly decreased the level of blood glucose, improved the HOMA‐IR, and increased the weight of testis and epididymis, as well as the sperm number and sperm motility. Furthermore, the pathologic changes in the spermatogenic tubules improved significantly with increased number of spermatogenic cells, the upregulation of PCNA and SIRT1 and suppression of cell apoptosis in the testes. Collectively, our study for the first time examined the effects of DNLP on the male reproductive system of STZ‐induced diabetic rats, and indicated that DNLP was protective against diabetes mellitus‐induced testis injury via increasing the proliferation, inhibiting cell apoptosis and upregulating SIRT1 expression in testicular spermatogenic cells.

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Section: Discussionmentioning

confidence: 99%

Dendrobium nobile Lindl polysaccharides improve testicular spermatogenic function in streptozotocin‐induced diabetic rats

Lei

Huo

Xie

et al. 2022

Molecular Reproduction Devel

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“…The strong and sharp absorption bands from 1200 to 1000 cm −1 were assigned to C-O-C stretching vibration of glycosidic bonds and the C-O-H group. Furthermore, the absorption at 1149, 1078, and 1019 cm −1 indicated a pyranose form of sugars, and the absorptions at 1078, and 1019 cm −1 indicated furanose form of sugars (Fan et al, 2020). In addition, the characteristic absorption band at 929 cm −1 revealed β-type glycosidic linkages, while 846 cm −1 was assigned to α-type glycosidic linkages (Dong et al, 2020;Huang et al, 2020).…”

Section: Analysis Of Ft-ir Spectra Of Apmentioning

confidence: 94%

A novel polysaccharide from plant fermentation extracts and its immunomodulatory activity in macrophage RAW264.7 cells

Yan

Liu

Yan

et al. 2021

Food and Agricultural Immunology

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Plant fermentation extracts (PFEs) produced from fermenting various fruits and vegetables by microorganisms have traditionally been consumed as healthy beverages in Asia. Herein, the immunomodulatory activities of a novel polysaccharide (AP) isolated from PFE were examined in RAW264.7 cells. The results showed that AP was composed of Rha, Xyl, Man and Glu in a molar ratio of 9.7:27.6:8.81:53.85 and the average molecular weight was 4.81 × 10 5 Da. Our data showed that AP could significantly enhance the phagocytic activity of RAW264.7 cells and their ability to release NO, TNF-α, IL-6 and IL-10. Furthermore, AP could significantly up-regulate the mRNA expression of iNOS, cytokines (TNF-α, IL-6, IL-10) and pattern recognition receptors (TLR2, MR, GR, CR3 and SR). Western blotting demonstrated that the regulation of NO and TNF-α were mediated through the NF-κB and MAPK signalling pathways. These findings helped to elucidate the immunomodulatory properties of the polysaccharide AP and PFE.

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“…They attributed this finding to the hydroxyl radical produced by plasma. In addition, Fan et al (2020) reported that treatment with nonthermal plasma could enhance the hydrophilicity of polysaccharide from Dendrobium nobile Lindl, possibly through an increase in the hydroxyl group (−OH) content in the molecule, resulting in the monosaccharide ring changing from β-pyran sugar to β-furan sugar. This phenomenon also improved the immune activity in vitro, especially the phagocytosis ability of RAW264.7, and promoted the secretion of cytokines, such as TNF-α, IL-6, and IL-1.…”

Section: Cp Modification Of Polysaccharidesmentioning

confidence: 99%

“…of 40% total reducing sugar; destroyed the amorphous and crystalline region; broken and separated into pieces; appeared carbonyl groupsrate of argon ionized gas of 3 L/min, output current of 8 mA and treatment time of 30 s. Increased the hydrophilicity of polysaccharide and the content of hydroxyl group in structure; the monosaccharide ring change from β-pyran sugar to β-furan sugar; increased the degree of cross-linking of surface molecules; promoted the expression of IL-1β at the transcriptional levelFan et al (2020) …”

mentioning

confidence: 99%

Application of nonthermal processing technologies in extracting and modifying polysaccharides: A critical review

Zhang

et al. 2021

Comp Rev Food Sci Food Safe

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Polysaccharides are natural polymer compounds widely distributed in plants, animals, and microorganisms, most of which have a broad spectrum of biological activities to promote human health. They could also be used as texture modifiers in food industry due to their excellent rheological and mechanical properties. Many researchers have shown that nonthermal processing technologies have numerous advantages, such as high extraction efficiency, short extraction time, and environmental friendliness, in the extraction of polysaccharides compared with the traditional extraction methods. Moreover, nonthermal technologies could effectively change the physicochemical properties and structural characteristics of polysaccharides to improve their biological activities or processing properties. Therefore, a comprehensive summary about the extraction and modification of polysaccharides by nonthermal technologies, including ultrasound, high hydrostatic pressure, pulsed electric fields, and cold plasma, was provided in this review. In particular, the underlying mechanisms, processing operations, and current application status of these technologies were discussed. In addition, the applications of combining nonthermal techniques with other technological methods in polysaccharide extraction and modification were briefly introduced.

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Effects of non-thermal plasma treatment on the polysaccharide from Dendrobium nobile Lindl. And its immune activities in vitro

Cited by 31 publications

References 36 publications

Dendrobium nobile Lindl polysaccharides improve testicular spermatogenic function in streptozotocin‐induced diabetic rats

Dendrobium nobile Lindl polysaccharides improve testicular spermatogenic function in streptozotocin‐induced diabetic rats

A novel polysaccharide from plant fermentation extracts and its immunomodulatory activity in macrophage RAW264.7 cells

Application of nonthermal processing technologies in extracting and modifying polysaccharides: A critical review

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