Effects of organic modifier and temperature on micellar liquid chromatography

Tomasella, Frank P.; Fett, Joseph J.; Love, L. J. Cline.

doi:10.1021/ac00005a018

Cited by 53 publications

(23 citation statements)

References 30 publications

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“…Some valuable experience (e.g. [144]) from column LC can be compiled to MEKC, e.g., the effect of organic modifiers ( and of temperature [147] on selectivity. However, there is one important difference between column LC and MEKC.…”

Section: Diagram 5 Sensible Settings For Initial Ce Experimentsmentioning

confidence: 99%

Strategies for capillary electrophoresis: Method development and validation for pharmaceutical and biological applications

1998

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This review is in support of the development of selective, reproducible and validated capillary electrophoretis (CE) methods. Focusing on pharmaceutical and biological applications, the successful use of CE is demonstrated by more than 800 references, mainly from 1994 until 1998. Approximately 80 recent reviews have been catalogued. These articles sum up the existing strategies for method development in CE, especially in the search for generally accepted concepts, but also looking for new, promising reagents and ideas. General strategies for method development were derived not only with regard to selectivity and efficiency, but also with regard to precision, short analysis time, limit of detection, sample pretreatment requirements and validation. Standard buffer recipes, surfactants used in micellar electrokinetic capillary chromatography (MEKC), chiral selectors, useful buffer additives, polymeric separation media, electroosmotic flow (EOF) modifiers, dynamic and permanent coatings, actions to deal with complex matrices and aspects of validation are collected in 20 tables. Detailed schemes for the development of MEKC methods and chiral separations, for optimizing separation efficiency, means of troubleshooting, and other important information for key decisions during method development are given in 19 diagrams. Method development for peptide and protein separations, possibilities to influence the EOF and how to stabilize it, as well as indirect detection are considered in special sections.

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Section: Diagram 5 Sensible Settings For Initial Ce Experimentsmentioning

confidence: 99%

Strategies for capillary electrophoresis: Method development and validation for pharmaceutical and biological applications

1998

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“…In MLC, solutes present in a mixture can partition between the bulk aqueous phase and the surfactant-modified stationary phase (P WS ) and between the bulk aqueous phase and the micellar aggregate present in the mobile phase (P WM ) (4,(10)(11)(12). In addition, for water insoluble solutes, partitioning can occur directly between the micelle solute species and the surfactant-coated stationary phase (P MS ) (11,13).…”

Section: Introductionmentioning

confidence: 99%

“…In addition, for water insoluble solutes, partitioning can occur directly between the micelle solute species and the surfactant-coated stationary phase (P MS ) (11,13). Since micellar systems can bind a variety of solutes as a result of favorable electrostatic, pi, H-bonding, hydrophobic, or a combination of such interactions, such mobile phases are much more versatile than any of the conventional mobile-phase systems.…”

Section: Introductionmentioning

confidence: 99%

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Study of the Separation Selectivity of a Group of Benzene and Naphthalene Derivatives in Micellar Liquid Chromatography

Marina

Jiménez

García

et al. 1996

Microchemical Journal

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“…Various approaches have been attempted to overcome this. The most common and effective one has been using small 13 percentage of organic solvent such as a short-chain alcohol as the eluent modifier and 3% 1-propanol is the best so far [143] although the breakdown of micelles limits the amount of alcohol which can be incorporated into the mobile phase [144][145][146]. It is believed that the presence of this small amount of alcohol in the mobile phase facilitates the mass transfer of the solute between the micelle, bulk eluent and stationary phase.…”

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confidence: 99%

Method development for high performance liquid chromatography: novel organic modifiers and column packing conditions

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This manuscript has been reproduced from the microfilm master. UMl films the text directly from the original or copy submitted. Thus, some thesis and dissertation copies are in typewriter face, while others may be from any type of computer printer.The quality of this reproduction is dependent upon the quality of the copy submitted. Broken or indistinct print, colored or poor quality illustrations and photographs, print bleedthrough, substandard margins, and improper alignment can adversely affect reproduction.In the unlikely event that the author did not send UMI a complete manuscript and there are missing pages, these will be noted. Also, if unauthorized copyright material had to be removed, a note will indicate the deletion.Oversize materials (e.g., maps, drawings, charts) are reproduced by sectioning the original, beguming at the upper left-hand comer and continuing from left to right in equal sections with small overlaps. Each original is also photographed in one exposure and is included in reduced form at the back of the book.Photographs included in the original manuscript have been reproduced xerographically in this copy. Higher quality 6" x 9" black and white photographic prints are available for any photographs or illustrations appearing in this copy for an additional charge. Contact UMI directly to order. 1996Signature was redacted for privacy.Signature was redacted for privacy.Signature was redacted for privacy. Dissertation OrganizationThis dissertation begins with a general introduction containing a literature review.References cited in the literature review are compiled in the reference section within the general introduction. This is then followed by four chapters all of which are in the format of research papers. They have been accepted, have been submitted or will soon be submitted for publication. A general conclusion section follows the four chapters. The second paper is similar to the publication version, although additional figures and tables have been added.Figures and tables are contained in the text of the paper at the appropriate location.References cited within each paper are listed after the conclusions of each paper. [70]. Indeed, it is amazing just to think about the popularity of HPLC from major components to trace analysis, from food, soil and water to poison, drug and trace evidence, from organic to inorganic analysis, firom natural to synthetic products, from peptides and proteins to RNA and DNA, from industry to clinics, from separation and pxirification to structure elucidation. The future of HPLC seems to be even brighter with gas chromatography, supercritical fluid chromatography, field flow fractionation and capillary electrophoresis to complement it. Higfai Performance Liquid Chromatography 4Although HPLC has become a well established technique, advances are still being made in the fundamental understanding of various separation mechanisms as well as improvements in equipment capabilities.In this dissertation, HPLC methods were developed for the separations of various org...

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Effects of organic modifier and temperature on micellar liquid chromatography

Cited by 53 publications

References 30 publications

Strategies for capillary electrophoresis: Method development and validation for pharmaceutical and biological applications

Strategies for capillary electrophoresis: Method development and validation for pharmaceutical and biological applications

Study of the Separation Selectivity of a Group of Benzene and Naphthalene Derivatives in Micellar Liquid Chromatography

Method development for high performance liquid chromatography: novel organic modifiers and column packing conditions

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