Suter, S.; W o k , R.; aumbach, M. M. I n Advances in human growftr hormone research: a symposium; Raiti, S., Ed.; US Dept. of HEW: Washington, DC. 1974; Publ. No. NIH (25) Protection of Laboratory Workers from Infectious Disease Transmitted by Blood and Tissue. NCCLS Document M29-P, Vol. 7, No. 9, 1987. (26) Munson, P. J. A user's guide to LIGAND: data analysis and curve-fitting for ligand binding experiment, 1983; available from Systex, Inc.; 74-612, pp 725-756.The role of an added organic modifier In micellar liquid chromatography is Investlgated. The change in equlllbrla caused by the addltlon of an organic modifier to the micellar moblle phase Is calculated. Thermodynamic property trends are examined In accordance wlth the three-phase model. Enthalpy-entropy compensation behavior Is tested for varylng amounts of added organic modifier. The change In retention mechanism as a resull of added organic modifler is described.

The liquid chromatographic analysis of drugs in urine through direct injection without any sample pretreatment was extended to micellar chromatography with nonionic surfactants, the Pinkerton ISRP column and the shielded hydrophobic phase (Hisep) column. The feasibility of using each was demonstrated through the determination of the diuretic, hydrochlorothiazide, in urine. Good separation, recovery, precision and linearity, and adequate limits of detection were obtained for this analysis with all three techniques. The advantages and limitations of the mobile phase approach of micellar chromatography and the two stationary phase approaches are discussed for the direct injection of urine as well as other biological fluids.

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